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Structure paper

TitleAlteration of the 2-Oxoacid Cosubstrate Selectivity in Deacetoxycephalosporin C Synthase: The Role of Arginine-258
Journal, issue, pagesJ. Biol. Chem., Vol. 276, Page 18290-, Year 2001
Publish dateJan 15, 2001 (structure data deposition date)
AuthorsLee, H.J. / Lloyd, M.D. / Harlos, K. / Clifton, I.J. / Baldwin, J.E. / Schofield, C.J.
External linksJ. Biol. Chem. / PubMed:11279000
MethodsX-ray diffraction
Resolution1.5 - 1.6 Å
Structure data

PDB-1hjf:
Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1hjg:
Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-FE2:
Unknown entry

ChemComp-COI:
2-OXO-4-METHYLPENTANOIC ACID

ChemComp-HOH:
WATER

ChemComp-KIV:
3-METHYL-2-OXOBUTANOIC ACID

Source
  • streptomyces clavuligerus (bacteria)
KeywordsOXIDOREDUCTASE / ALTERNATIVE 2-OXOACIDS / CEPHEM ANTIBIOTIC BIOSYNTHESIS / CHEMICAL COSUBSTRATE RESCUE / CO-SUBSTRATE SELECTIVITY / 2- OXOGLUTARATE-DEPENDENT OXYGENASE

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