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Structure paper

TitleX-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Journal, issue, pagesNat. Struct. Biol., Vol. 6, Page 432-436, Year 1999
Publish dateFeb 24, 1999 (structure data deposition date)
AuthorsUitdehaag, J.C. / Mosi, R. / Kalk, K.H. / van der Veen, B.A. / Dijkhuizen, L. / Withers, S.G. / Dijkstra, B.W.
External linksNat. Struct. Biol. / PubMed:10331869
MethodsX-ray diffraction
Resolution1.81 - 2.09 Å
Structure data

PDB-1cxk:
COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-1cxl:
COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
Method: X-RAY DIFFRACTION / Resolution: 1.81 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • bacillus circulans (bacteria)
KeywordsTRANSFERASE / GLYCOSYL HYDROLASE FAMILY 13 / ALPHA-AMYLASE FAMILY / SUBSTRATE COMPLEX / MALTONONAOSE / GLYCOSYLTRANSFERASE / INTERMEDIATE COMPLEX / 4- DEOXYMALTOTRIOSE

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