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TitleStructure determination of echovirus 1.
Journal, issue, pagesActa Crystallogr D Biol Crystallogr, Vol. 54, Issue Pt 6 Pt 2, Page 1261-1272, Year 1998
Publish dateNov 1, 1998
AuthorsD J Filman / M W Wien / J A Cunningham / J M Bergelson / J M Hogle /
PubMed AbstractThe atomic structure of echovirus 1 (a member of the enterovirus genus of the picornavirus family) has been determined using cryo-crystallography and refined to 3.55 A resolution. Echovirus 1 ...The atomic structure of echovirus 1 (a member of the enterovirus genus of the picornavirus family) has been determined using cryo-crystallography and refined to 3.55 A resolution. Echovirus 1 crystallizes in space group P22121 with a = 352.45, b = 472.15 and c = 483.20 A. The crystals contain one full virus particle in the asymmetric unit allowing for 60-fold noncrystallographic symmetry averaging. The diffraction pattern shows strong pseudo-B-centering with reflections with h + l = 2n + 1 being systematically weak or absent below about 6 A resolution. The size of the unit cell and presence of pseudo-B-centering placed strong constraints on the allowed packing of the icosahedral particle in the crystal lattice. These constraints greatly facilitated the determination of the orientation and position of the virus by reducing the dimensionality of the search, but interactions between the crystallographic and noncrystallographic symmetries rendered the choice of space group ambiguous until very late in the structure determination. This structure determination provides a striking example of the power of packing analysis in molecular replacement and illustrates how subtle interactions between crystallographic and noncrystallographic symmetries can be resolved.
External linksActa Crystallogr D Biol Crystallogr / PubMed:10089503
MethodsX-ray diffraction
Resolution3.55 Å
Structure data

PDB-1ev1:
ECHOVIRUS 1
Method: X-RAY DIFFRACTION / Resolution: 3.55 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID

ChemComp-MYR:
MYRISTIC ACID

ChemComp-HOH:
WATER

Source
  • human echovirus 1
KeywordsVIRUS / VIRAL COAT PROTEIN / CAPSID / PICORNAVIRUS / ECHOVIRUS / Icosahedral virus

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