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Structure paper

TitlePhage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA.
Journal, issue, pagesScience, Vol. 386, Issue 6717, Page eadq3977, Year 2024
Publish dateOct 4, 2024
AuthorsMax E Wilkinson / David Li / Alex Gao / Rhiannon K Macrae / Feng Zhang /
PubMed AbstractReverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are ...Reverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are generally unknown. Here, we show that in the DRT2 defense system, the reverse transcriptase binds a neighboring pseudoknotted noncoding RNA. Upon bacteriophage infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that reconstitute a promoter and open reading frame, allowing expression of a toxic repetitive protein and an abortive infection response. Biochemical reconstitution of this activity and cryo-electron microscopy provide a molecular basis for repeat synthesis. Gene synthesis from a noncoding RNA is a previously unknown mode of genetic regulation in prokaryotes.
External linksScience / PubMed:39208082
MethodsEM (single particle)
Resolution2.91 - 3.17 Å
Structure data

45085

9c0i

EMDB-45085, PDB-9c0i:
Structure of the DRT2 reverse transcriptase in complex with its non-coding RNA
Method: EM (single particle) / Resolution: 2.91 Å

45086

9c0j

EMDB-45086, PDB-9c0j:
Structure of the elongating DRT2 reverse transcriptase in complex with its non-coding RNA and dNTPs
Method: EM (single particle) / Resolution: 3.17 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-TTP:
THYMIDINE-5'-TRIPHOSPHATE

Source
  • klebsiella pneumoniae (bacteria)
KeywordsTRANSFERASE/DNA/RNA / reverse transcriptase / rolling circle amplification / phage defense / TRANSFERASE-DNA-RNA complex

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