Journal: Cell / Year: 2017 Title: Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. Authors: Xuelian Zhao / Katie A Howell / Shihua He / Jennifer M Brannan / Anna Z Wec / Edgar Davidson / Hannah L Turner / Chi-I Chiang / Lin Lei / J Maximilian Fels / Hong Vu / Sergey Shulenin / ...Authors: Xuelian Zhao / Katie A Howell / Shihua He / Jennifer M Brannan / Anna Z Wec / Edgar Davidson / Hannah L Turner / Chi-I Chiang / Lin Lei / J Maximilian Fels / Hong Vu / Sergey Shulenin / Ashley N Turonis / Ana I Kuehne / Guodong Liu / Mi Ta / Yimeng Wang / Christopher Sundling / Yongli Xiao / Jennifer S Spence / Benjamin J Doranz / Frederick W Holtsberg / Andrew B Ward / Kartik Chandran / John M Dye / Xiangguo Qiu / Yuxing Li / M Javad Aman / Abstract: While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an ...While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an immunization-elicited macaque antibody (CA45) that clamps the internal fusion loop with the N terminus of the ebolavirus glycoproteins (GPs) and potently neutralizes Ebola, Sudan, Bundibugyo, and Reston viruses. CA45, alone or in combination with an antibody that blocks receptor binding, provided full protection against all pathogenic ebolaviruses in mice, guinea pigs, and ferrets. Analysis of memory B cells from the immunized macaque suggests that elicitation of broadly neutralizing antibodies (bNAbs) for ebolaviruses is possible but difficult, potentially due to the rarity of bNAb clones and their precursors. Unexpectedly, germline-reverted CA45, while exhibiting negligible binding to full-length GP, bound a proteolytically remodeled GP with picomolar affinity, suggesting that engineered ebolavirus vaccines could trigger rare bNAb precursors more robustly. These findings have important implications for developing pan-ebolavirus vaccine and immunotherapeutic cocktails.
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Apr 18, 2017
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Feb 14, 2018
Processing site: RCSB / Status: Released
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