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Yorodumi- EMDB-9956: Cryo-EM structure of the C2S2M2L2-type PSII-LHCII supercomplex fr... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-9956 | |||||||||||||||
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Title | Cryo-EM structure of the C2S2M2L2-type PSII-LHCII supercomplex from Chlamydomonas reihardtii | |||||||||||||||
Map data | ||||||||||||||||
Sample |
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Keywords | PhotosystemII / supercomplex / PHOTOSYNTHESIS | |||||||||||||||
Function / homology | Function and homology information thylakoid light-harvesting complex / photosystem II antenna complex / nonphotochemical quenching / PSII associated light-harvesting complex II / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization ...thylakoid light-harvesting complex / photosystem II antenna complex / nonphotochemical quenching / PSII associated light-harvesting complex II / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / photosynthetic electron transport chain / response to herbicide / photosystem II / photosynthesis, light reaction / chloroplast thylakoid membrane / phosphate ion binding / photosynthetic electron transport in photosystem II / chlorophyll binding / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / response to light stimulus / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / protein domain specific binding / mRNA binding / heme binding / metal ion binding Similarity search - Function | |||||||||||||||
Biological species | Chlamydomonas reinhardtii (plant) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||||||||
Authors | Sheng X / Watanabe A | |||||||||||||||
Funding support | China, Japan, 4 items
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Citation | Journal: Nat Plants / Year: 2019 Title: Structural insight into light harvesting for photosystem II in green algae. Authors: Xin Sheng / Akimasa Watanabe / Anjie Li / Eunchul Kim / Chihong Song / Kazuyoshi Murata / Danfeng Song / Jun Minagawa / Zhenfeng Liu / Abstract: Green algae and plants rely on light-harvesting complex II (LHCII) to collect photon energy for oxygenic photosynthesis. In Chlamydomonas reinhardtii, LHCII molecules associate with photosystem II ...Green algae and plants rely on light-harvesting complex II (LHCII) to collect photon energy for oxygenic photosynthesis. In Chlamydomonas reinhardtii, LHCII molecules associate with photosystem II (PSII) to form various supercomplexes, including the CSML type, which is the largest PSII-LHCII supercomplex in algae and plants that is presently known. Here, we report high-resolution cryo-electron microscopy (cryo-EM) maps and structural models of the CSML and CS supercomplexes from C. reinhardtii. The CS supercomplex contains an LhcbM1-LhcbM2/7-LhcbM3 heterotrimer in the strongly associated LHCII, and the LhcbM1 subunit assembles with CP43 through two interfacial galactolipid molecules. The loosely and moderately associated LHCII trimers interact closely with the minor antenna complex CP29 to form an intricate subcomplex bound to CP47 in the CSML supercomplex. A notable direct pathway is established for energy transfer from the loosely associated LHCII to the PSII reaction centre, as well as several indirect routes. Structure-based computational analysis on the excitation energy transfer within the two supercomplexes provides detailed mechanistic insights into the light-harvesting process in green algae. | |||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_9956.map.gz | 202.6 MB | EMDB map data format | |
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Header (meta data) | emd-9956-v30.xml emd-9956.xml | 39.6 KB 39.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_9956_fsc.xml | 13.5 KB | Display | FSC data file |
Images | emd_9956.png | 54.7 KB | ||
Filedesc metadata | emd-9956.cif.gz | 9.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-9956 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9956 | HTTPS FTP |
-Validation report
Summary document | emd_9956_validation.pdf.gz | 654.7 KB | Display | EMDB validaton report |
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Full document | emd_9956_full_validation.pdf.gz | 654.2 KB | Display | |
Data in XML | emd_9956_validation.xml.gz | 13.3 KB | Display | |
Data in CIF | emd_9956_validation.cif.gz | 18.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-9956 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-9956 | HTTPS FTP |
-Related structure data
Related structure data | 6kadMC 9955C 6kacC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_9956.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
+Entire : The C2S2M2L2-type PSII-LHCII supercomplex
+Supramolecule #1: The C2S2M2L2-type PSII-LHCII supercomplex
+Macromolecule #1: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #2: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #3: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #4: Photosystem II protein D1
+Macromolecule #5: Photosystem II CP47 reaction center protein
+Macromolecule #6: Photosystem II CP43 reaction center protein
+Macromolecule #7: Photosystem II D2 protein
+Macromolecule #8: Cytochrome b559 subunit alpha
+Macromolecule #9: Cytochrome b559 subunit beta
+Macromolecule #10: Photosystem II reaction center protein H
+Macromolecule #11: Photosystem II reaction center protein I
+Macromolecule #12: Photosystem II reaction center protein J
+Macromolecule #13: Photosystem II reaction center protein K
+Macromolecule #14: Photosystem II reaction center protein L
+Macromolecule #15: Photosystem II reaction center protein M
+Macromolecule #16: Oxygen-evolving enhancer protein 1, chloroplastic
+Macromolecule #17: Chlorophyll a-b binding protein CP29
+Macromolecule #18: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #19: Photosystem II reaction center protein T
+Macromolecule #20: Photosystem II reaction center protein Ycf12
+Macromolecule #21: Photosystem II reaction center W protein, chloroplastic
+Macromolecule #22: 4.1 kDa photosystem II subunit
+Macromolecule #23: Photosystem II reaction center protein Z
+Macromolecule #24: CHLOROPHYLL B
+Macromolecule #25: CHLOROPHYLL A
+Macromolecule #26: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+Macromolecule #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+Macromolecule #28: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+Macromolecule #29: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #30: CA-MN4-O5 CLUSTER
+Macromolecule #31: FE (II) ION
+Macromolecule #32: CHLORIDE ION
+Macromolecule #33: PHEOPHYTIN A
+Macromolecule #34: BETA-CAROTENE
+Macromolecule #35: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #36: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #37: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #38: BICARBONATE ION
+Macromolecule #39: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+Macromolecule #40: PROTOPORPHYRIN IX CONTAINING FE
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 6.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.31 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |