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- EMDB-71632: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CC... -

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Basic information

Entry
Database: EMDB / ID: EMD-71632
TitleThe cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex
Map data
Sample
  • Complex: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex
    • Protein or peptide: DNA-directed RNA polymerase subunit alpha
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase subunit beta'
    • Protein or peptide: DNA-directed RNA polymerase subunit omega
    • Protein or peptide: RNA polymerase sigma factor RpoD
    • DNA: DNA (55-MER)-non template
    • DNA: DNA (43-MER)-template
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
KeywordsCCNA_03891-CCNA_01149 promoter complex / DriD / Caulobacter / RNA Polymerase / Transcription / TRANSCRIPTION-DNA complex
Function / homology
Function and homology information


sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / cytoplasm
Similarity search - Function
RNA polymerase sigma factor 70, non-essential domain / Sigma-70, non-essential region / RNA polymerase sigma factor 70, region 1.1 / Sigma-70 factor, region 1.1 superfamily / Sigma-70 factor, region 1.1 / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / : / RNA polymerase sigma-70 region 1.2 ...RNA polymerase sigma factor 70, non-essential domain / Sigma-70, non-essential region / RNA polymerase sigma factor 70, region 1.1 / Sigma-70 factor, region 1.1 superfamily / Sigma-70 factor, region 1.1 / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / : / RNA polymerase sigma-70 region 1.2 / Sigma-70 factor, region 1.2 / RNA polymerase sigma-70 region 3 / Sigma-70 region 3 / Sigma-70 factors family signature 2. / RNA polymerase sigma-70 / RNA polymerase sigma-70 region 4 / Sigma-70, region 4 / RNA polymerase sigma-70 region 2 / RNA polymerase sigma-70 like domain / Sigma-70 region 2 / RNA polymerase sigma factor, region 2 / RNA polymerase sigma factor, region 3/4-like / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase beta subunit, bacterial-type / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb6 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RPB6/omega subunit-like superfamily / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
RNA polymerase sigma factor RpoD / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta
Similarity search - Component
Biological speciesCaulobacter vibrioides NA1000 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.54 Å
AuthorsSingh RR / Schumacher MA
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM130290 United States
Citation
Journal: Sci Adv / Year: 2026
Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD.
Authors: Rajiv R Singh / Amani Chinni / Emily Cannistraci / Raul Salinas / Sunny Yadav / Kevin Gozzi / Maria A Schumacher /
Abstract: DNA damage repair mechanisms are vital for cell survival. In the bacterium, , DriD is the master regulator of a unique, noncanonical DNA damage pathway. DriD binding to ssDNA, produced upon DNA ...DNA damage repair mechanisms are vital for cell survival. In the bacterium, , DriD is the master regulator of a unique, noncanonical DNA damage pathway. DriD binding to ssDNA, produced upon DNA damage, stimulates its ability to activate transcription from several promoters involved in DNA damage responses. However, the mechanism by which DriD interfaces with the RNAP holoenzyme to activate transcription from its multiple promoters has been unclear. Here, we describe cryo-EM structures of DriD-ssDNA bound to RNAP-holoenzyme and three DriD-regulated promoters. Each subunit of homodimeric DriD contains an DNA binding -terminal winged helix-turn-helix (wHTH) connected to WYL domains by a linker 3-helix bundle (3HB) module. The structures reveal a mechanism of assembly on promoters whereby DriD's 3HBs bind the RNAP α-CTD and β domains, anchoring the RNAP-holoenzyme to regulated promoters. The 3HBs form autoinhibitory contacts with DNABDs in apo DriD and therefore acts as an ssDNA-driven trigger domain, switching between DNABD-bound apo and RNAP-bound forms upon ssDNA-mediated activation. Thus, the structures reveal a unique transcription activation mechanism, likely conserved among the large family of homodimeric WYL activators.
#1: Journal: To Be Published
Title: The cryo-EM structure of C. crescentus DriD-ssDNA-RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter transcription activation complex
Authors: Singh RR / Schumacher MA
#2: Journal: To Be Published
Title: The cryo-EM structure of C. crescentus DriD-ssDNA-RNAP-Sigma73-didA promoter transcription activation complex
Authors: Singh RR / Schumacher MA
#3: Journal: To Be Published
Title: The cryo-EM structure of C. crescentus DriD-ssDNA-RNAP-Sigma73-bapE promoter transcription activation complex
Authors: Singh RR / Schumacher MA
History
DepositionJul 7, 2025-
Header (metadata) releaseFeb 25, 2026-
Map releaseFeb 25, 2026-
UpdateApr 15, 2026-
Current statusApr 15, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_71632.png

Downloads & links

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Map

FileDownload / File: emd_71632.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 384 pix.
= 414.72 Å		1.08 Å/pix.
x 384 pix.
= 414.72 Å		1.08 Å/pix.
x 384 pix.
= 414.72 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.08 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0033
Minimum - Maximum-0.034164384 - 0.07076755
Average (Standard dev.)0.000022486021 (±0.001341611)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 414.72003 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_71632_msk_1.map
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Half map: #2

Fileemd_71632_half_map_1.map
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Half map: #1

Fileemd_71632_half_map_2.map
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Sample components

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Entire : The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CC...

EntireName: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex
Components
  • Complex: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex
    • Protein or peptide: DNA-directed RNA polymerase subunit alpha
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase subunit beta'
    • Protein or peptide: DNA-directed RNA polymerase subunit omega
    • Protein or peptide: RNA polymerase sigma factor RpoD
    • DNA: DNA (55-MER)-non template
    • DNA: DNA (43-MER)-template
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CC...

SupramoleculeName: The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#7
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)

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Macromolecule #1: DNA-directed RNA polymerase subunit alpha

MacromoleculeName: DNA-directed RNA polymerase subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)
Molecular weightTheoretical: 37.315523 KDa
SequenceString: MIERNWNELI RPEKPQIETG ADATRKARIV AEPLERGFGV TLGNALRRVL LSSLQGAAVT AIQIDGVVHE FSSLEGVRED VVDIVLNIK QLAVRMHAEG PKRMTLRATG PGPVTAGQIE TPADIEILNP DHVLCTLDDG ASVRMEFTVN NGKGYVPADR N RPEDAPIG ...String:
MIERNWNELI RPEKPQIETG ADATRKARIV AEPLERGFGV TLGNALRRVL LSSLQGAAVT AIQIDGVVHE FSSLEGVRED VVDIVLNIK QLAVRMHAEG PKRMTLRATG PGPVTAGQIE TPADIEILNP DHVLCTLDDG ASVRMEFTVN NGKGYVPADR N RPEDAPIG LIAVDALYSP VKRVAYRVEP TRQGQSLDYD KLILEVETNG AVTPVDAVAY AARILQDQLQ IFITFEEPKA KS ADESKPE LPFNPALLKK VDELELSVRS ANCLKNDNIV YIGDLIQKTE AEMLRTPNFG RKSLNEIKEV LAGMGLHLGM DVP NWPPEN IEDLAKKFED QI

UniProtKB: DNA-directed RNA polymerase subunit alpha

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)
Molecular weightTheoretical: 151.085609 KDa
SequenceString: MAQSFTGKKR IRKSFGRIPE AVQMPNLIEV QRSSYEQFLQ RETRPGLRRD EGVEAVFKSV FPIKDFNERA VLEYVSYEFE EPKYDVEEC IQRDMTFAAP LKVKLRLIVF ETEEETGARS VKDIKEQDVY MGDIPLMTDK GTFIVNGTER VIVSQMHRSP G VFFDHDKG ...String:
MAQSFTGKKR IRKSFGRIPE AVQMPNLIEV QRSSYEQFLQ RETRPGLRRD EGVEAVFKSV FPIKDFNERA VLEYVSYEFE EPKYDVEEC IQRDMTFAAP LKVKLRLIVF ETEEETGARS VKDIKEQDVY MGDIPLMTDK GTFIVNGTER VIVSQMHRSP G VFFDHDKG KTHASGKLLF AARVIPYRGS WLDFEFDAKD IVYVRIDRRR KLPATTFLYA LGMDGEEILT TFYDVVPFEK RS GGWATPY KPERWRGVKP EFPLVDADTG EEVAPAGTKI TARQAKKFAD GGLKTLLLAP EALTGRYLAR DAVNMATGEI YAE AGDELD VTSIQALADQ GFSTIDVLDI DHVTVGAYMR NTLRVDKNAI REDALFDIYR VMRPGEPPTV EAAEAMFKSL FFDA ERYDL SSVGRVKMNM RLEQDVSDEV RILRKEDVLA VLKVLVGLRD GRGEIDDIDN LGNRRVRSVG ELLENQYRVG LLRME RAIK ERMSSVDIDT VMPHDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH KRRLSALGPG GLTRERAGFE VRDVHP THY GRICPIETPE GPNIGLINSL ATHARVNKYG FIESPYRRVK DGKPQDEVVY MSAMEESKHV IAQSNIKVAE GEIVEDL VP GRINGEPTLL QKETVDLMDV SPRQVVSVAA ALIPFLENDD ANRALMGSNM QRQAVPLVQS DAPLVGTGME AVVARDSG A VVIAKRTGVV EQIDGTRIVI RATEETDPAR SGVDIYRMSK FQRSNQSTCI NQRPLVKVGD RIVAGDIIAD GPSTELGEL ALGRNALVAF MPWNGYNFED SILISERIVR DDVFTSIHIE EFEVMARDTK LGPEEITRDI PNVGEEALRN LDEAGIVAIG AEVQPGDIL VGKVTPKGES PMTPEEKLLR AIFGEKASDV RDTSLRLPPG VAGTIVDVRV FNRHGVDKDE RALAIERAEI D RLGKDRDD EFAILNRNIS GRLKELLIGK VALSGPKGLS RGEITAEGLA QVASGLWWQI ALEDEKAMGE LESLRRLFDE NR KRLDRRF EDKVDKLQRG DELPPGVMKM VKVFVAVKRK LQPGDKMAGR HGNKGVISRI LPIEDMPFLA DGTHVDVVLN PLG VPSRMN VGQIFETHLG WACANLGKQI TNLLEDWQQG GQKQALVERL TEIYGPDEEL PDTEEGLVEL ARNLGKGVPI ATPV FDGAR MDDIEGHLEM AGVNKSGQSI LFDGLTGEQF KRPVTVGYIY MLKLHHLVDD KIHARSIGPY SLVTQQPLGG KAQFG GQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVAG RTKVYESIVR GDDTFEAGIP ESFNVLVKEM RSLGLNVELE NS

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #3: DNA-directed RNA polymerase subunit beta'

MacromoleculeName: DNA-directed RNA polymerase subunit beta' / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)
Molecular weightTheoretical: 154.467 KDa
SequenceString: MNQEVLNIFN PVQAAPTFDQ IRISLASPEK IRSWSFGEIK KPETINYRTF KPERDGLFCA RIFGPTKDYE CLCGKYKRMK YKGIICEKC GVEVTLARVR RERMGHIELA SPVAHIWFLK SLPSRIAMML DMPLKDIERV LYFEYYIVTE PGLTPLKQHQ L LSEDDYMR ...String:
MNQEVLNIFN PVQAAPTFDQ IRISLASPEK IRSWSFGEIK KPETINYRTF KPERDGLFCA RIFGPTKDYE CLCGKYKRMK YKGIICEKC GVEVTLARVR RERMGHIELA SPVAHIWFLK SLPSRIAMML DMPLKDIERV LYFEYYIVTE PGLTPLKQHQ L LSEDDYMR AQEEYGDDSF TAEIGAEAIQ NLLKAIDLEK EAERLREELS GTVSDMKQKK FSKRLKILEA FQESGNRPEW MV LTVVPVI PPELRPLVPL DGGRFATSDL NDLYRRVINR NNRLKRLIEL RAPDIIIRNE KRMLQESVDA LFDNGRRGRV ITG ANKRPL KSLADMLKGK QGRFRQNLLG KRVDYSGRSV IVVGPELKLH ECGLPKKMAL ELFKPFIYAR LDAKGLSGTV KQSK RMVER EQPQVWDILE EVIREHPVLL NRAPTLHRLG IQAFEPKLIE GKAIQLHPLV CAAFNADFDG DQMAVHVPLS LEAQL EARV LMMSTNNILS PANGRPIIVP SQDIVLGLYY LSVARDGEPG EGKIFADLGE IEAAMDAGVV SLHAKIKARH TEMTPE GVL LRKVIDTTPG RMKIAALLPH HPQIGHRLIE KALTKKEIGN LIDIVYRHCG QKATVIFADK VMGLGFKEAA KAGISFG KD DIIIPVRKTA IVEETRKLAE EYEQQYADGL ITKGEKYNKV VDAWAKATDR VADEMMAELQ MKHKDENGRE KEINAIYM M AHSGARGSQA QMKQLGGMRG LMAKPSGEII ETPIVSNFKE GLTVQEYFNS THGARKGLAD TALKTANSGY LTRRLVDVA QDCIIVEEDC GTTKGITLRA VVEGGDVLVS LGSRVLGRFT AEDVKDPGTG ELVVPADTYI DENIADAIEA AVVQSVKVRS VLTCEAKIG VCGACYGRDL ARGTPVNIGE AVGVIAAQSI GEPGTQLTMR TFHIGGTAQV AEQSFFEASN EGTVRVIGPT V VGSDGALV IMSRNTSVSV LVDGKERETY KPPYGARLRV KDGDLVKRGQ RLGDWDPYTT PIITEVAGKI RAEDLVDGLS IR EEVDEAT GIAQRVVADW RTSARGSDLR PAMGVLSEDG SYKRLSNGGE ARYLLSAGAI LSVADGDEVK PGEVIARIPT EGA KTRDIT GGLPRVAELF EARRPKDCAV IAEMDGRVEF GKDYKNKRRI KITPDVDADG NQPEAVEFLI PKGKHIAVHD GDYI TKGEY IIDGNPDPHD ILRILGVEAL ANFLVDEIQE VYRLQGVPIN DKHIETIVRQ MLQKVEILEP GDTGLIKGDH LDKPE FDKE QEKAIARGGR PAVTQPVLLG ITKASLQTKS FISAASFQET TRVLTEASVH GKTDTLEGLK ENVIVGRLIP AGTGSY LRS LQRVAAKRDE QLAQQREDAM EPLPAEIALS DAE

UniProtKB: DNA-directed RNA polymerase subunit beta'

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Macromolecule #4: DNA-directed RNA polymerase subunit omega

MacromoleculeName: DNA-directed RNA polymerase subunit omega / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)
Molecular weightTheoretical: 13.289844 KDa
SequenceString:
MARVTVEDCV EKVPNRFALV LLSAHRARGI SAGAALMVDR DNDKNPVVAL REIADDVIDH EGLKEHLIST LQRVDEHTEA EEEAETLAL LADPSHMQMS ELELVRALQS DRDGGQEERY

UniProtKB: DNA-directed RNA polymerase subunit omega

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Macromolecule #5: RNA polymerase sigma factor RpoD

MacromoleculeName: RNA polymerase sigma factor RpoD / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria) / Strain: ML2315 (BSL1)
Molecular weightTheoretical: 72.770172 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSNNSSAETE APETTGGDGP LLDLTDAGVK KFIKQAKARG YVTMDELNKV LPSEEVSPDA IEDTLAMLSE MGVNVVEAEE DAENAEGGE VATRDENTTV IASDKPAAYD RTDDPVRMYL REMGSVELLS REGEIAIAKR IEAGRDTMIR GLCESALTFE A IMVWREEL ...String:
MSNNSSAETE APETTGGDGP LLDLTDAGVK KFIKQAKARG YVTMDELNKV LPSEEVSPDA IEDTLAMLSE MGVNVVEAEE DAENAEGGE VATRDENTTV IASDKPAAYD RTDDPVRMYL REMGSVELLS REGEIAIAKR IEAGRDTMIR GLCESALTFE A IMVWREEL GTGRILLREV IDLEGTYAAI NGVAAQPAAE DDEGPAEPVD DEAGEAKAEG AEGEDEDDFD DGAGPTVSAM EG ELREGVM AILDAIASEF EAFRKLQDKL VGSRLKGEDL SDADRKAYEG LSATIIQHLK TLKLNNNRIE ALVEQLYAIN KRL IGLEGR LLRLADSYGI SRGEFLKAYF GSELNPTWSE QVKAMGVRWT KFVENDSQSV TDIRSEIAAL ATETGVPIDD YRRI VQTVQ KGEREARQAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV DKFEYRRGYK FSTYATWWIR QAITR SIAD QARTIRIPVH MIETINKIVR TSRQMLHEIG REPTPEELAE KLAMPLEKVR KVLKIAKEPI SLETPIGDEE DSHLGD FIE DKNAILPIDA AIQSNLRETT TRVLASLTPR EERVLRMRFG IGMNTDHTLE EVGQQFSVTR ERIRQIEAKA LRKLKHP SR SRKLRSFLDS

UniProtKB: RNA polymerase sigma factor RpoD

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Macromolecule #6: DNA (55-MER)-non template

MacromoleculeName: DNA (55-MER)-non template / type: dna / ID: 6 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria)
Molecular weightTheoretical: 30.196248 KDa
SequenceString: (DC)(DA)(DT)(DG)(DC)(DC)(DG)(DC)(DC)(DC) (DG)(DT)(DC)(DA)(DT)(DA)(DC)(DG)(DA)(DC) (DC)(DG)(DT)(DT)(DT)(DC)(DT)(DG)(DT) (DC)(DG)(DC)(DA)(DA)(DT)(DG)(DC)(DG)(DC) (DG) (DG)(DA)(DC)(DA)(DA)(DT) ...String:
(DC)(DA)(DT)(DG)(DC)(DC)(DG)(DC)(DC)(DC) (DG)(DT)(DC)(DA)(DT)(DA)(DC)(DG)(DA)(DC) (DC)(DG)(DT)(DT)(DT)(DC)(DT)(DG)(DT) (DC)(DG)(DC)(DA)(DA)(DT)(DG)(DC)(DG)(DC) (DG) (DG)(DA)(DC)(DA)(DA)(DT)(DC)(DT) (DT)(DC)(DC)(DA)(DT)(DC)(DA)(DT)(DC)(DG) (DC)(DC) (DC)(DC)(DT)(DG)(DG)(DA)(DG) (DA)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DG)(DA) (DC)(DC)(DT) (DT)(DC)(DG)(DA)(DT)(DG) (DA)(DC)(DC)(DC)(DG)(DC)(DT)(DT)(DC)(DG) (DC)(DC)(DT)

GENBANK: GENBANK: CP001340.1

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Macromolecule #7: DNA (43-MER)-template

MacromoleculeName: DNA (43-MER)-template / type: dna / ID: 7 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Caulobacter vibrioides NA1000 (bacteria)
Molecular weightTheoretical: 30.89468 KDa
SequenceString: (DA)(DG)(DG)(DC)(DG)(DA)(DA)(DG)(DC)(DG) (DG)(DG)(DT)(DC)(DA)(DT)(DC)(DG)(DA)(DA) (DG)(DG)(DT)(DC)(DC)(DT)(DT)(DG)(DG) (DG)(DT)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG) (DG) (DG)(DC)(DG)(DA)(DT)(DG) ...String:
(DA)(DG)(DG)(DC)(DG)(DA)(DA)(DG)(DC)(DG) (DG)(DG)(DT)(DC)(DA)(DT)(DC)(DG)(DA)(DA) (DG)(DG)(DT)(DC)(DC)(DT)(DT)(DG)(DG) (DG)(DT)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG) (DG) (DG)(DC)(DG)(DA)(DT)(DG)(DA)(DT) (DG)(DG)(DA)(DA)(DG)(DA)(DT)(DT)(DG)(DT) (DC)(DC) (DG)(DC)(DG)(DC)(DA)(DT)(DT) (DG)(DC)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA) (DC)(DG)(DG) (DT)(DC)(DG)(DT)(DA)(DT) (DG)(DA)(DC)(DG)(DG)(DG)(DC)(DG)(DG)(DC) (DA)(DT)(DG)

GENBANK: GENBANK: CP001340.1

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Macromolecule #8: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 8 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #9: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 9 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
20.0 mMC4H11NO3Tris-HCl
5.0 mMMgCl2Magnesium Chloride
100.0 mMNaClSodium Chloride
1.0 mMC2H6OS1

Details: 20 mM Tris-cl, 5 mM MgCl2, 100 mM NaCl, 1 mM BME
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Support film - Film thickness: 50 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 300 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 293.15 K / Instrument: LEICA EM GP
Details3 uM of the reconstituted complex

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 58.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. 4.2.1) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: Ab-Initio Reconstruction
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.54 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5) / Number images used: 150644
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

7ye1


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-9pgh:
The cryo-EM structure of C. crescentus RNAP-Sigma73-CCNA_03891/CCNA_01149 promoter complex

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