+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-60607 | |||||||||
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Title | A local Cryo-EM structure of Bitter taste receptor TAS2R14 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | GPCR / MEMBRANE PROTEIN | |||||||||
Function / homology | Function and homology information bitter taste receptor activity / taste receptor activity / detection of chemical stimulus involved in sensory perception of bitter taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / G protein-coupled receptor signaling pathway / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.15 Å | |||||||||
Authors | Yuan Q / Duan J / Tao L / Xu EH | |||||||||
Funding support | China, 1 items
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Citation | Journal: Cell Res / Year: 2024 Title: Bitter taste receptor TAS2R14 activation and G protein assembly by an intracellular agonist. Authors: Lele Tao / Dongxue Wang / Qingning Yuan / Fenghui Zhao / Yu Zhang / Tianyuan Du / Shiyi Shen / H Eric Xu / Yi Li / Dehua Yang / Jia Duan / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_60607.map.gz | 96.9 MB | EMDB map data format | |
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Header (meta data) | emd-60607-v30.xml emd-60607.xml | 13.6 KB 13.6 KB | Display Display | EMDB header |
Images | emd_60607.png | 34.6 KB | ||
Filedesc metadata | emd-60607.cif.gz | 5.3 KB | ||
Others | emd_60607_half_map_1.map.gz emd_60607_half_map_2.map.gz | 95.6 MB 95.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-60607 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-60607 | HTTPS FTP |
-Validation report
Summary document | emd_60607_validation.pdf.gz | 816.4 KB | Display | EMDB validaton report |
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Full document | emd_60607_full_validation.pdf.gz | 816 KB | Display | |
Data in XML | emd_60607_validation.xml.gz | 13.3 KB | Display | |
Data in CIF | emd_60607_validation.cif.gz | 15.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60607 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60607 | HTTPS FTP |
-Related structure data
Related structure data | 9iiwMC 9iixC 9ij9C 9ijaC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_60607.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.824 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_60607_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_60607_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : local refinement of TAS2R14
Entire | Name: local refinement of TAS2R14 |
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Components |
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-Supramolecule #1: local refinement of TAS2R14
Supramolecule | Name: local refinement of TAS2R14 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Taste receptor type 2 member 14
Macromolecule | Name: Taste receptor type 2 member 14 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 36.20007 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MGGVIKSIFT FVLIVEFIIG NLGNSFIALV NCIDWVKGRK ISSVDRILTA LAISRISLVW LIFGSWCVSV FFPALFATEK MFRMLTNIW TVINHFSVWL ATGLGTFYFL KIANFSNSIF LYLKWRVKKV VLVLLLVTSV FLFLNIALIN IHINASINGY R RNKTCSSD ...String: MGGVIKSIFT FVLIVEFIIG NLGNSFIALV NCIDWVKGRK ISSVDRILTA LAISRISLVW LIFGSWCVSV FFPALFATEK MFRMLTNIW TVINHFSVWL ATGLGTFYFL KIANFSNSIF LYLKWRVKKV VLVLLLVTSV FLFLNIALIN IHINASINGY R RNKTCSSD SSNFTRFSSL IVLTSTVFIF IPFTLSLAMF LLLIFSMWKH RKKMQHTVKI SGDASTKAHR GVKSVITFFL LY AIFSLSF FISVWTSERL EENLIILSQV MGMAYPSCHS CVLILGNKKL RQASLSVLLW LRYMFKDGEP SGHKEFRESS UniProtKB: Taste receptor type 2 member 14 |
-Macromolecule #2: 4-methyl-N-[(2M)-2-(1H-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)p...
Macromolecule | Name: 4-methyl-N-[(2M)-2-(1H-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine type: ligand / ID: 2 / Number of copies: 2 / Formula: A1AEI |
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Molecular weight | Theoretical: 321.261 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.04 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 15.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 18.0 µm / Nominal defocus min: 8.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.15 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 354147 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |