Journal: Structure / Year: 2011 Title: Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses. Authors: Xuekui Yu / Peng Ge / Jiansen Jiang / Ivo Atanasov / Z Hong Zhou / Abstract: Unlike the multishelled viruses in the Reoviridae, cytoplasmic polyhedrosis virus (CPV) is single shelled, yet stable and fully capable of carrying out functions conserved within Reoviridae. Here, we ...Unlike the multishelled viruses in the Reoviridae, cytoplasmic polyhedrosis virus (CPV) is single shelled, yet stable and fully capable of carrying out functions conserved within Reoviridae. Here, we report a 3.1 Å resolution cryo electron microscopy structure of CPV and derive its atomic model, consisting of 60 turret proteins (TPs), 120 each of capsid shell proteins (CSPs) and large protrusion proteins (LPPs). Two unique segments of CSP contribute to CPV's stability: an inserted protrusion domain interacting with neighboring proteins, and an N-anchor tying up CSPs together through strong interactions such as β sheet augmentation. Without the need to interact with outer shell proteins, LPP retains only the N-terminal two-third region containing a conserved helix-barrel core and interacts exclusively with CSP. TP is also simplified, containing only domains involved in RNA capping. Our results illustrate how CPV proteins have evolved in a coordinative manner to economically carry out their conserved functions.
History
Deposition
Jan 14, 2011
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Header (metadata) release
Jan 27, 2011
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Map release
Jun 23, 2011
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Update
Apr 2, 2012
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Current status
Apr 2, 2012
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Name: cytoplasmic polyhedrosis virus (CPV) / type: sample / ID: 1000 / Details: CPV particles were purified from polyhedra. / Oligomeric state: Icosahedral particle / Number unique components: 5
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Supramolecule #1: Bombyx mori cypovirus 1
Supramolecule
Name: Bombyx mori cypovirus 1 / type: virus / ID: 1 / Name.synonym: cytoplasmic polyhedrosis virus / NCBI-ID: 110829 / Sci species name: Bombyx mori cypovirus 1 / Database: NCBI / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No / Syn species name: cytoplasmic polyhedrosis virus
Host (natural)
Organism: Bombyx mori (domestic silkworm) / synonym: INVERTEBRATES
Virus shell
Shell ID: 1 / Diameter: 730 Å
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Experimental details
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Structure determination
Method
negative staining, cryo EM
Processing
single particle reconstruction
Aggregation state
particle
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Sample preparation
Concentration
3 mg/mL
Buffer
pH: 7.4 / Details: 10mM PBS
Staining
Type: NEGATIVE / Details: Sample was not stained
Grid
Details: holey carbon grid
Vitrification
Cryogen name: ETHANE / Chamber humidity: 40 % / Chamber temperature: 90 K / Instrument: OTHER / Method: Blot for 3 seconds before plunging
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Electron microscopy
Microscope
FEI TITAN KRIOS
Temperature
Average: 90 K
Alignment procedure
Legacy - Astigmatism: objective lens astigmatism was corrected at 150,000 magnification Legacy - Electron beam tilt params: 0
Details
Low dose
Date
Jul 12, 2010
Image recording
Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: NIKON SUPER COOLSCAN 9000 / Digitization - Sampling interval: 6.35 µm / Number real images: 996 / Average electron dose: 25 e/Å2 / Bits/pixel: 16
Tilt angle min
0
Tilt angle max
0
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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