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- EMDB-50271: Additional cryo-EM structure of cardiac amyloid AL59 - bent polymorph -
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Open data
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Basic information
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Title | Additional cryo-EM structure of cardiac amyloid AL59 - bent polymorph | |||||||||
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![]() | systemic AL amyloid fibril / PROTEIN FIBRIL | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
![]() | Schulte T / Speranzini V / Chaves-Sanjuan A / Milazzo M / Ricagno S | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Additional cryo-EM structure of cardiac amyloid AL59 - bent polymorph Authors: Schulte T / Chaves-Sanjuan A / Speranzini V / Sicking K / Milazzo M / Mazzini G / Rognoni P / Caminito S / Milani P / Marabelli C / Corbelli A / Diomede L / Fiordaliso F / Anastasia L / ...Authors: Schulte T / Chaves-Sanjuan A / Speranzini V / Sicking K / Milazzo M / Mazzini G / Rognoni P / Caminito S / Milani P / Marabelli C / Corbelli A / Diomede L / Fiordaliso F / Anastasia L / Pappone C / Merlini G / Bolognesi M / Nuvolone M / Fernandez-Busnadiego R / Palladini G / Ricagno S | |||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.889 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Monoclonal immunoglobulin light chains (LC), fibrillar form
Entire | Name: Monoclonal immunoglobulin light chains (LC), fibrillar form |
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Components |
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-Supramolecule #1: Monoclonal immunoglobulin light chains (LC), fibrillar form
Supramolecule | Name: Monoclonal immunoglobulin light chains (LC), fibrillar form type: tissue / ID: 1 / Parent: 0 / Macromolecule list: #1 Details: Amyloid fibrils extracted ex vivo from cardiac tissue of AL patient |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Monoclonal immunoglobulin light chains (LC)
Macromolecule | Name: Monoclonal immunoglobulin light chains (LC) / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 13.923335 KDa |
Sequence | String: SFELTQPSSV SVSPGQTANI TCSGGYLGET YRSWYQQKPG QSPVLVIYQS SKRPSGIPGR FSGSNSGNTA TLTISGTQPL DEADYFCQA WDFTSVVFGG GTKLTVLGQP KAAPSVTLFP PSSEELQANK ATL |
-Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 5 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Number real images: 2049 / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 120000 |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 4.9162 Å Applied symmetry - Helical parameters - Δ&Phi: -0.7351 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 23010 |
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Startup model | Type of model: NONE |
Final angle assignment | Type: NOT APPLICABLE |