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- EMDB-40474: Leishmania tarentolae propionyl-CoA carboxylase (alpha-6-beta-6) -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Leishmania tarentolae propionyl-CoA carboxylase (alpha-6-beta-6) | |||||||||
![]() | Leishmania tarentolae propionyl-CoA carboxylase (alpha-6-beta-6) | |||||||||
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![]() | Multienzyme complex / carboxylase / LIGASE | |||||||||
Function / homology | ![]() urea carboxylase activity / propionyl-CoA carboxylase / propionyl-CoA carboxylase activity / methylcrotonoyl-CoA carboxylase activity / acetyl-CoA carboxylase complex / lipid catabolic process / mitochondrion / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Lee JKJ / Liu YT / Hu JJ / Aphasizheva I / Aphasizhev R / Zhou ZH | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CryoEM reveals oligomeric isomers of a multienzyme complex and assembly mechanics. Authors: Jane K J Lee / Yun-Tao Liu / Jason J Hu / Inna Aphasizheva / Ruslan Aphasizhev / Z Hong Zhou / ![]() Abstract: Propionyl-CoA carboxylase (PCC) is a multienzyme complex consisting of up to six α-subunits and six β-subunits. Belonging to a metabolic pathway converging on the citric acid cycle, it is present ...Propionyl-CoA carboxylase (PCC) is a multienzyme complex consisting of up to six α-subunits and six β-subunits. Belonging to a metabolic pathway converging on the citric acid cycle, it is present in most forms of life and irregularities in its assembly lead to serious illness in humans, known as propionic acidemia. Here, we report the cryogenic electron microscopy (cryoEM) structures and assembly of different oligomeric isomers of endogenous PCC from the parasitic protozoan (LtPCC). These structures and their statistical distribution reveal the mechanics of PCC assembly and disassembly at equilibrium. We show that, in solution, endogenous LtPCC β-subunits form stable homohexamers, to which different numbers of α-subunits attach. Sorting LtPCC particles into seven classes (i.e., oligomeric formulae αβ, αβ, αβ, αβ, αβ, αβ, αβ) enables formulation of a model for PCC assembly. Our results suggest how multimerization regulates PCC enzymatic activity and showcase the utility of cryoEM in revealing the statistical mechanics of reaction pathways. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 202 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16 KB 16 KB | Display Display | ![]() |
Images | ![]() | 106.8 KB | ||
Filedesc metadata | ![]() | 6 KB | ||
Others | ![]() ![]() | 171.6 MB 171.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8sgzMC ![]() 8sgxC ![]() 8sgyC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Leishmania tarentolae propionyl-CoA carboxylase (alpha-6-beta-6) | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map 1
File | emd_40474_half_map_1.map | ||||||||||||
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Annotation | Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 2
File | emd_40474_half_map_2.map | ||||||||||||
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Annotation | Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Leishmania tarentolae propionyl-CoA carboxylase
Entire | Name: Leishmania tarentolae propionyl-CoA carboxylase |
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Components |
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-Supramolecule #1: Leishmania tarentolae propionyl-CoA carboxylase
Supramolecule | Name: Leishmania tarentolae propionyl-CoA carboxylase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: propionyl-CoA carboxylase
Macromolecule | Name: propionyl-CoA carboxylase / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 73.277656 KDa |
Sequence | String: VLVANRGEIA CRVMATCRRL GIKTVAVYST ADEQAKHVKV ADESVCIGPP ASVESYLCID KIVDACKKTG AQAVHPGYGF LSENGEFQS ALQKNNIVFV GPDAHSIESM GDKIESKRLA QRAGVTCIPG FIGEVKTHED LLRFAREIGY PVMIKASGGG G GKGMRVAY ...String: VLVANRGEIA CRVMATCRRL GIKTVAVYST ADEQAKHVKV ADESVCIGPP ASVESYLCID KIVDACKKTG AQAVHPGYGF LSENGEFQS ALQKNNIVFV GPDAHSIESM GDKIESKRLA QRAGVTCIPG FIGEVKTHED LLRFAREIGY PVMIKASGGG G GKGMRVAY NDTQCVEYYD MCREEAKAAF HSDKMLVERF IDHPRHIEIQ VIADRRGNTV YLPERECSIQ RRNQKVIEEA PS VLLDATT RKAMGEEAVA MARAVQYVSA GTVENVVNPQ KQFYFLEMNT RLQVEHPITE EITGVDLVEQ MLRAAADLPL SIT QDDITI NGHATECRVY AEDPMKNYFP SIGRLTMYQE PTGAGVRCDS GIIEGSQISV YYDPLICKLS TWGRDRAECI GRME KALDE YVIRGLRHNI CLLRDVVTEP RYRSGSITTN YLQEQYPNGF KKAELTAEEM QLMYEVAACV HLKRERLHYT QGTAP SERQ LYLSVGAGQE GETPVYVRYL DDSHFEIGAS KHGPFRKMEV VWKASYPIIR VKDGEAETVL QFWGTNEVTY GMQMRG TTF DVNVMSDLQS TLAHFVPITE ATTNTKQILS PMPGVIVAIK VQPGQMVVAG EELLTLEAMK MRNKIHAQAD GKVKEVK VK LGATVEDNEV LVELE UniProtKB: propionyl-CoA carboxylase |
-Macromolecule #2: Propionyl-coa carboxylase beta chain, putative
Macromolecule | Name: Propionyl-coa carboxylase beta chain, putative / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 53.587285 KDa |
Sequence | String: PTAAEDLRHK KKRLTAMERV QLFCDPGTFR ERDALVEHEC HNFGMEKRKV PGDGFITGTG KVFGRPVFLF SHDFTVFGGS LSRTNAAKV VRIMEEAAKI GVPVIGFNDS GGARIHEGVD SLAGYADIFL RNTLFSGVIP QISVIMGPCA GGAVYSPAIT D FTFMVETS ...String: PTAAEDLRHK KKRLTAMERV QLFCDPGTFR ERDALVEHEC HNFGMEKRKV PGDGFITGTG KVFGRPVFLF SHDFTVFGGS LSRTNAAKV VRIMEEAAKI GVPVIGFNDS GGARIHEGVD SLAGYADIFL RNTLFSGVIP QISVIMGPCA GGAVYSPAIT D FTFMVETS SYMFVTGPEV VSAVGGKLVT KDELGGPHVH ATKSGVSAGT FPNDIVAMAQ LRRLYSYLPL SNRDPVPVLP TA DERYRDV SSLNTVVPTE VKEAYDMRDV IYPVIDHDSF FEIQPQFAKN IICGFARVEG RSVCIIANQP KVQAGVLDID SSV KGARMV RFADAFNIPI ITFVDVPGFL PGVQQEYGGI IRHGAKLLYA YAEATVPKVT IITRKAYGGA YDVMSSKHLR GDSN YAWPH AEIAVMGAAG ACKLLYSKET AEQQAQRIAD YEKTFCTPLS AARKGFVDAV IDPSETRMRV CEDLERLARK QLQNP WKKH GNIPL UniProtKB: Propionyl-coa carboxylase beta chain, putative |
-Macromolecule #3: 5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL
Macromolecule | Name: 5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL type: ligand / ID: 3 / Number of copies: 6 / Formula: BTI |
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Molecular weight | Theoretical: 228.311 Da |
Chemical component information | ![]() ChemComp-BTI: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 15859 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |