[English] 日本語
![](img/lk-miru.gif)
- EMDB-3942: Structure of the chloroplast ribosome with chl-RRF and hibernatio... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-3942 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of the chloroplast ribosome with chl-RRF and hibernation-promoting factor (Focused refinement of LSU) | |||||||||
![]() | Postprocessed sharpened map that has been resampled onto the map used in the refinement of the coordinates | |||||||||
![]() |
| |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
![]() | Perez Borema A / Aibara S / Paul B / Tobiasson V / Kimanius D / Forsberg BO / Wallden K / Lindahl E / Amunts A | |||||||||
![]() | ![]() Title: Structure of the chloroplast ribosome with chl-RRF and hibernation-promoting factor. Authors: Annemarie Perez Boerema / Shintaro Aibara / Bijoya Paul / Victor Tobiasson / Dari Kimanius / Björn O Forsberg / Karin Wallden / Erik Lindahl / A Amunts / ![]() ![]() Abstract: Oxygenic photosynthesis produces oxygen and builds a variety of organic compounds, changing the chemistry of the air, the sea and fuelling the food chain on our planet. The photochemical reactions ...Oxygenic photosynthesis produces oxygen and builds a variety of organic compounds, changing the chemistry of the air, the sea and fuelling the food chain on our planet. The photochemical reactions underpinning this process in plants take place in the chloroplast. Chloroplasts evolved ~1.2 billion years ago from an engulfed primordial diazotrophic cyanobacterium, and chlororibosomes are responsible for synthesis of the core proteins driving photochemical reactions. Chlororibosomal activity is spatiotemporally coupled to the synthesis and incorporation of functionally essential co-factors, implying the presence of chloroplast-specific regulatory mechanisms and structural adaptation of the chlororibosome. Despite recent structural information, some of these aspects remained elusive. To provide new insights into the structural specialities and evolution, we report a comprehensive analysis of the 2.9-3.1 Å resolution electron cryo-microscopy structure of the spinach chlororibosome in complex with its recycling factor and hibernation-promoting factor. The model reveals a prominent channel extending from the exit tunnel to the chlororibosome exterior, structural re-arrangements that lead to increased surface area for translocon binding, and experimental evidence for parallel and convergent evolution of chloro- and mitoribosomes. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 13.4 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 19.3 KB 19.3 KB | Display Display | ![]() |
Images | ![]() | 264.5 KB | ||
Masks | ![]() | 282.6 MB | ![]() | |
Others | ![]() ![]() ![]() ![]() ![]() | 249.2 MB 25.9 MB 224.3 MB 225.1 MB 225.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Postprocessed sharpened map that has been resampled onto the map used in the refinement of the coordinates | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: unsharpened map that has been resampled onto the...
File | emd_3942_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | unsharpened map that has been resampled onto the map used in the refinement of the coordinates | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: Postprocessed sharpened map that has not been resampled...
File | emd_3942_additional_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Postprocessed sharpened map that has not been resampled onto the map used in the refinement of the coordinates | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: unsharpened map that has not been resampled onto...
File | emd_3942_additional_3.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | unsharpened map that has not been resampled onto the map used in the refinement of the coordinates | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: halfmap 1
File | emd_3942_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | halfmap 1 | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: halfmap 2
File | emd_3942_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | halfmap 2 | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : Chloroplast ribosome in complex with RRF - Focussed refinement on LSU
Entire | Name: Chloroplast ribosome in complex with RRF - Focussed refinement on LSU |
---|---|
Components |
|
-Supramolecule #1: Chloroplast ribosome in complex with RRF - Focussed refinement on LSU
Supramolecule | Name: Chloroplast ribosome in complex with RRF - Focussed refinement on LSU type: complex / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 4.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 130300 |
---|---|
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |