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Yorodumi- EMDB-39288: Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at post-... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-39288 | |||||||||
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Title | Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at post-state 2 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | protein structure / ANTIVIRAL PROTEIN | |||||||||
Biological species | metagenome (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.07 Å | |||||||||
Authors | Zhang H / Deng Z / Li X | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at post-state 2 Authors: Zhang H / Deng Z / Li X | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_39288.map.gz | 97.3 MB | EMDB map data format | |
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Header (meta data) | emd-39288-v30.xml emd-39288.xml | 16.5 KB 16.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_39288_fsc.xml | 9.9 KB | Display | FSC data file |
Images | emd_39288.png | 82 KB | ||
Filedesc metadata | emd-39288.cif.gz | 5.9 KB | ||
Others | emd_39288_half_map_1.map.gz emd_39288_half_map_2.map.gz | 95.5 MB 95.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-39288 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-39288 | HTTPS FTP |
-Related structure data
Related structure data | 8yhe M: atomic model generated by this map |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_39288.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.95 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_39288_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_39288_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : protein structure
Entire | Name: protein structure |
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Components |
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-Supramolecule #1: protein structure
Supramolecule | Name: protein structure / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: metagenome (others) |
-Macromolecule #1: protein structure
Macromolecule | Name: protein structure / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 22.255604 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MAKTMKKIYV TMKTLSPLYT GEVRREDKEA AQKRVNFPVR KTATNKVLIP FKGALRSALE IMLKAKGENV CDTGESRARP CGRCVTCSL FGSMGRAGRA SVDFLISNDT KEQIVRESTH LRIERQTKSA SDTFKGEEVI EGATFTATIT ISNPQEKDLS L IQSALKFI ...String: MAKTMKKIYV TMKTLSPLYT GEVRREDKEA AQKRVNFPVR KTATNKVLIP FKGALRSALE IMLKAKGENV CDTGESRARP CGRCVTCSL FGSMGRAGRA SVDFLISNDT KEQIVRESTH LRIERQTKSA SDTFKGEEVI EGATFTATIT ISNPQEKDLS L IQSALKFI EENGIGGWLN KGYGRVSFEV KSEDVATDRF LK |
-Macromolecule #2: protein structure
Macromolecule | Name: protein structure / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 27.139533 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKEIKGILES ITGFSIPLDN GEYALYPAGR HLRGAIGYIA FNLDLPISSK FLDFDFDDII FRDLLPISKC GKIFYPEKNS NSLKCPSCN EIYGSSVLRN IMARGLSYKE VIEGKKYRLS IIVKDEKYLN EMEAIIRYIL SYGIYLGNKV SKGYGKFKIK E YSIVDILP ...String: MKEIKGILES ITGFSIPLDN GEYALYPAGR HLRGAIGYIA FNLDLPISSK FLDFDFDDII FRDLLPISKC GKIFYPEKNS NSLKCPSCN EIYGSSVLRN IMARGLSYKE VIEGKKYRLS IIVKDEKYLN EMEAIIRYIL SYGIYLGNKV SKGYGKFKIK E YSIVDILP VKDSEVLLLS DAIIDNGEKD IVFSKKEISS SKFEIIRKRG KAKGDIIRDN NHNGFYIGKY GGLGFGEIIS LK |
-Macromolecule #3: protein structure
Macromolecule | Name: protein structure / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 70.465875 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MIKFIGGASK VTGSAFLLET GAKILIDCGI EQEKGIEKDN NEIIEKKINE IGKADICILT HAHLDHSGLV PLLVKKRKVN KIISTPATK ELCRLLFNDF QRIQEENNDI PLYSYDDIES SFEIWDEIDD RNTIELFDTK ITFYNNSHII GSVSVFIETH N GNYLFSGD ...String: MIKFIGGASK VTGSAFLLET GAKILIDCGI EQEKGIEKDN NEIIEKKINE IGKADICILT HAHLDHSGLV PLLVKKRKVN KIISTPATK ELCRLLFNDF QRIQEENNDI PLYSYDDIES SFEIWDEIDD RNTIELFDTK ITFYNNSHII GSVSVFIETH N GNYLFSGD IGSKLQQLMD YPPDMPDGNV DYLILESTYG NKSHDSSDRD RLLEIAKTTC ENGGKVLIPS FAIGRLQEVL YT FSNYNFN FPVYIDSPMG SKVTNLIKEY NIYLKKKLRR LSITDDLFNN KYIAINTSNQ SKELSNSKEP AVIISASGML EGG RILNHL EQIKNDENST LIFVGYQAQN TRGRKILDGE EKVRCRIEKL NSFSAHADQD ELIDYIERLK YTPYKVFLVH GEKE QREIL AKRIISKKIR VELPENYSQG KEILIEKKVV LNINTDNMCN FASYRLMPFS GFIVEKDDRI EINDKNWFDM IWNEE YNKM RSQIVAEDFS TDQNEDSMAL PDMSHDKIIE NIEYLFNIKI LSKNRIKEFW EEFCKGQKAA IKYITQVHRK NPNTGR RNW NPPEGDFTDN EIEKLYETAY NTLLSLIKYD KNKVYNILIN FNPKL |
-Macromolecule #4: RNA (46-MER)
Macromolecule | Name: RNA (46-MER) / type: rna / ID: 4 / Number of copies: 1 |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 15.300997 KDa |
Sequence | String: GUUAAAACUC UUCUCAUGCU GGAUUCGAAA UUAGGUGCGC UUCGCGUU |
-Macromolecule #5: RNA (29-MER)
Macromolecule | Name: RNA (29-MER) / type: rna / ID: 5 / Number of copies: 1 |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 16.713082 KDa |
Sequence | String: GAACACCCAA UAGCGAAGCG CACCUAAUUU CGAAUCCAGC AUGAGAAGCU AA |
-Macromolecule #6: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 6 / Number of copies: 9 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | JEOL CRYO ARM 300 |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm |