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- EMDB-37767: Cryo-EM structure of human ABC Transporter ABCC4 with E1202Q muta... -

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Basic information

Entry
Database: EMDB / ID: EMD-37767
TitleCryo-EM structure of human ABC Transporter ABCC4 with E1202Q mutation from Biortus
Map data
Sample
  • Organelle or cellular component: Human atp-binding cassette sub-family cmember 4 (Protein from Homo sapiens)
KeywordsHuman atp-binding cassette sub-family cmember 4 (Protein from Homo sapiens) / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.74 Å
AuthorsHan T / Hu Y
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Cryo-EM structure of human ABC Transporter ABCC4 with E1202Q mutation from Biortus
Authors: Han T / Hu Y
History
DepositionOct 13, 2023-
Header (metadata) releaseNov 1, 2023-
Map releaseNov 1, 2023-
UpdateApr 10, 2024-
Current statusApr 10, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_37767.png

Downloads & links

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Map

FileDownload / File: emd_37767.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.74 Å/pix.
x 480 pix.
= 356.64 Å		0.74 Å/pix.
x 480 pix.
= 356.64 Å		0.74 Å/pix.
x 480 pix.
= 356.64 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.743 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.068
Minimum - Maximum-0.23491146 - 0.47276714
Average (Standard dev.)-0.0002260265 (±0.0071717487)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 356.63998 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_37767_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_37767_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_37767_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human atp-binding cassette sub-family cmember 4 (Protein from Hom...

EntireName: Human atp-binding cassette sub-family cmember 4 (Protein from Homo sapiens)
Components
  • Organelle or cellular component: Human atp-binding cassette sub-family cmember 4 (Protein from Homo sapiens)

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Supramolecule #1: Human atp-binding cassette sub-family cmember 4 (Protein from Hom...

SupramoleculeName: Human atp-binding cassette sub-family cmember 4 (Protein from Homo sapiens)
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: NITROGEN

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 55.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.4 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.74 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 247122
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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