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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Mouse Fc epsilon RI in complex with mIgE Fc | |||||||||
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![]() | IgE / high-affinity IgE receptor / allergy / IMMUNE SYSTEM | |||||||||
Function / homology | ![]() Platelet Adhesion to exposed collagen / IgE receptor activity / Fc-epsilon receptor I complex / serotonin secretion / Fc epsilon receptor (FCERI) signaling / Dectin-2 family / Fc receptor mediated stimulatory signaling pathway / Role of LAT2/NTAL/LAB on calcium mobilization / T cell differentiation involved in immune response / negative regulation of mast cell apoptotic process ...Platelet Adhesion to exposed collagen / IgE receptor activity / Fc-epsilon receptor I complex / serotonin secretion / Fc epsilon receptor (FCERI) signaling / Dectin-2 family / Fc receptor mediated stimulatory signaling pathway / Role of LAT2/NTAL/LAB on calcium mobilization / T cell differentiation involved in immune response / negative regulation of mast cell apoptotic process / high-affinity IgE receptor activity / GPVI-mediated activation cascade / FCERI mediated MAPK activation / Fc-gamma receptor III complex / mast cell apoptotic process / mast cell activation / FCERI mediated Ca+2 mobilization / serotonin secretion by platelet / positive regulation of interleukin-3 production / FCERI mediated NF-kB activation / Cell surface interactions at the vascular wall / neutrophil activation involved in immune response / positive regulation of mast cell cytokine production / Fc-gamma receptor signaling pathway / positive regulation of mast cell degranulation / regulation of platelet activation / positive regulation of type III hypersensitivity / IgE binding / positive regulation of type IIa hypersensitivity / regulation of release of sequestered calcium ion into cytosol / positive regulation of protein localization to cell surface / : / leukotriene biosynthetic process / positive regulation of type I hypersensitivity / interleukin-3-mediated signaling pathway / positive regulation of granulocyte macrophage colony-stimulating factor production / IgG binding / mast cell degranulation / phagocytosis, engulfment / positive regulation of interleukin-4 production / antigen processing and presentation of exogenous peptide antigen via MHC class I / Fc-epsilon receptor signaling pathway / positive regulation of interleukin-10 production / cellular response to low-density lipoprotein particle stimulus / regulation of immune response / immunoglobulin mediated immune response / positive regulation of phagocytosis / positive regulation of calcium-mediated signaling / neutrophil chemotaxis / Neutrophil degranulation / SH2 domain binding / osteoclast differentiation / establishment of localization in cell / protein localization to plasma membrane / integrin-mediated signaling pathway / phosphoprotein binding / calcium-mediated signaling / receptor internalization / positive regulation of interleukin-6 production / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of immune response / positive regulation of tumor necrosis factor production / cell surface receptor signaling pathway / endosome / defense response to bacterium / immune response / protein heterodimerization activity / membrane raft / external side of plasma membrane / innate immune response / protein kinase binding / cell surface / signal transduction / protein homodimerization activity / identical protein binding / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.71 Å | |||||||||
![]() | Zhang Z / Yui M / Ohto U / Shimizu T | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Mouse Fc epsilon RI in complex withMouse Fc epsilon RI in complex with mIgE Fc mIgE Fc Authors: Zhang Z / Yui M / Ohto U / Shimizu T | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.8 KB 18.8 KB | Display Display | ![]() |
Images | ![]() | 85 KB | ||
Filedesc metadata | ![]() | 6 KB | ||
Others | ![]() ![]() ![]() | 26.5 MB 49 MB 49 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8k7tMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.245 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: #1
File | emd_36941_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36941_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_36941_half_map_2.map | ||||||||||||
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Density Histograms |
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Sample components
-Entire : Mouse Fc epsilon RI in complex with mIgE Fc
Entire | Name: Mouse Fc epsilon RI in complex with mIgE Fc |
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Components |
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-Supramolecule #1: Mouse Fc epsilon RI in complex with mIgE Fc
Supramolecule | Name: Mouse Fc epsilon RI in complex with mIgE Fc / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: High affinity immunoglobulin epsilon receptor subunit alpha
Macromolecule | Name: High affinity immunoglobulin epsilon receptor subunit alpha type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 31.646197 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MVTGRSAQLC LALLFMSLDV ILTATEKSVL TLDPPWIRIF TGEKVTLSCY GNNHLQMNST TKWIHNGTVS EVNSSHLVIV SATVQDSGK YICQKQGLFK SKPVYLNVTQ DWLLLQTSAD MVLVHGSFDI RCHGWKNWNV RKVIYYRNDH AFNYSYESPV S IREATLND ...String: MVTGRSAQLC LALLFMSLDV ILTATEKSVL TLDPPWIRIF TGEKVTLSCY GNNHLQMNST TKWIHNGTVS EVNSSHLVIV SATVQDSGK YICQKQGLFK SKPVYLNVTQ DWLLLQTSAD MVLVHGSFDI RCHGWKNWNV RKVIYYRNDH AFNYSYESPV S IREATLND SGTYHCKGYL RQVKYESDKF RIAVVKAYKC KYYWLQLIFP LLVAILFAVD TGLLLSTEEQ FKSVLEIQKT GK YKKVETE LLTENLYFQG DYKDDDDKHH HHHHHH UniProtKB: High affinity immunoglobulin epsilon receptor subunit alpha |
-Macromolecule #2: High affinity immunoglobulin epsilon receptor subunit gamma
Macromolecule | Name: High affinity immunoglobulin epsilon receptor subunit gamma type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 11.890856 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MISAVILFLL LLVEQAAALG EPQLCYILDA VLFLYGIVLT LLYCRLKIQV RKAAIASREK ADAVYTGLNT RSQETYETLK HEKPPQWSH PQFEKEQKLI SEEDL UniProtKB: High affinity immunoglobulin epsilon receptor subunit gamma |
-Macromolecule #3: High affinity immunoglobulin epsilon receptor subunit beta
Macromolecule | Name: High affinity immunoglobulin epsilon receptor subunit beta type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 27.166141 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MDTENRSRAD LALPNPQESS SAPDIELLEA SPAKAAPPKQ TWRTFLKKEL EFLGATQILV GLICLCFGTI VCSVLYVSDF DEEVLLLYK LGYPFWGAVL FVLSGFLSII SERKNTLYLV RGSLGANIVS SIAAGTGIAM LILNLTNNFA YMNNCKNVTE D DGCFVASF ...String: MDTENRSRAD LALPNPQESS SAPDIELLEA SPAKAAPPKQ TWRTFLKKEL EFLGATQILV GLICLCFGTI VCSVLYVSDF DEEVLLLYK LGYPFWGAVL FVLSGFLSII SERKNTLYLV RGSLGANIVS SIAAGTGIAM LILNLTNNFA YMNNCKNVTE D DGCFVASF TTELVLMMLF LTILAFCSAV LFTIYRIGQE LESKKVPDDR LYEELNVYSP IYSELEDKGE TSSPVDSEQK LI SEEDL UniProtKB: High affinity immunoglobulin epsilon receptor subunit beta |
-Macromolecule #4: IgE Fc
Macromolecule | Name: IgE Fc / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 40.369723 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: METGLRWLLL VAVLKGVQCV RPVNITDPTL ELLHSSCDPN AFHSTIQLYC FIYGHILNDV SVSWLMDDRE ITDTLAQTVL IKEEGKLAS TCSKLNITEQ QWMSESTFTC KVTSQGVDYL AHTRRCPDHE PRGVITYLIP PSPLDLYQNG APKLTCLVVD L ESEKNVNV ...String: METGLRWLLL VAVLKGVQCV RPVNITDPTL ELLHSSCDPN AFHSTIQLYC FIYGHILNDV SVSWLMDDRE ITDTLAQTVL IKEEGKLAS TCSKLNITEQ QWMSESTFTC KVTSQGVDYL AHTRRCPDHE PRGVITYLIP PSPLDLYQNG APKLTCLVVD L ESEKNVNV TWNQEKKTSV SASQWYTKHH NNATTSITSI LPVVAKDWIE GYGYQCIVDH PDFPKPIVRS ITKTPGQRSA PE VYVFPPP EEESEDKRTL TCLIQNFFPE DISVQWLGDG KLISNSQHST TTPLKSNGSN QGFFIFSRLE VAKTLWTQRK QFT CQVIHE ALQKPRKLEK TISTSLGNTS LRPSHHHHHH |
-Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 7 / Number of copies: 7 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 61.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.71 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 91911 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |