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- EMDB-36888: Cryo-EM structure of Kaposi's Sarcoma-Associated Herpesvirus-G Pr... -
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Open data
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Basic information
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Title | Cryo-EM structure of Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Receptor (KSHV-GPCR)in complex with CXC chemokine CXCL1 | |||||||||
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![]() | Kaposi's Sarcoma Herpesvirus GPCR / KSHV-GPCR / chemokine / VIRAL PROTEIN | |||||||||
Function / homology | ![]() CXCR chemokine receptor binding / C-C chemokine receptor activity / C-C chemokine binding / chemokine-mediated signaling pathway / Chemokine receptors bind chemokines / chemokine activity / cell cycle / Interleukin-10 signaling / G protein-coupled serotonin receptor binding / enzyme activator activity ...CXCR chemokine receptor binding / C-C chemokine receptor activity / C-C chemokine binding / chemokine-mediated signaling pathway / Chemokine receptors bind chemokines / chemokine activity / cell cycle / Interleukin-10 signaling / G protein-coupled serotonin receptor binding / enzyme activator activity / neutrophil chemotaxis / calcium-mediated signaling / growth factor activity / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G protein-coupled acetylcholine receptor signaling pathway / G-protein activation / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste / specific granule lumen / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / chemotaxis / G-protein beta-subunit binding / Inactivation, recovery and regulation of the phototransduction cascade / heterotrimeric G-protein complex / antimicrobial humoral immune response mediated by antimicrobial peptide / G alpha (12/13) signalling events / extracellular vesicle / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / tertiary granule lumen / GTPase binding / retina development in camera-type eye / Ca2+ pathway / nervous system development / phospholipase C-activating G protein-coupled receptor signaling pathway / positive regulation of cytosolic calcium ion concentration / cell cortex / G alpha (i) signalling events / actin cytoskeleton organization / G alpha (s) signalling events / G alpha (q) signalling events / cellular response to lipopolysaccharide / Ras protein signal transduction / cell population proliferation / Extra-nuclear estrogen signaling / intracellular signal transduction / inflammatory response / immune response / G protein-coupled receptor signaling pathway / negative regulation of cell population proliferation / cell division / lysosomal membrane / signaling receptor binding / GTPase activity / centrosome / synapse / Neutrophil degranulation / protein-containing complex binding / GTP binding / signal transduction / extracellular space / extracellular exosome / extracellular region / membrane / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.01 Å | |||||||||
![]() | Liu YZ / Liu AJ | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Cryo-EM structure of Kaposi's Sarcoma Herpesvirus GPCR (KSHV-GPCR)in complex with CXCL1 Authors: Liu YZ / Liu AJ | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 117.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.7 KB 17.7 KB | Display Display | ![]() |
Images | ![]() | 43.6 KB | ||
Filedesc metadata | ![]() | 6 KB | ||
Others | ![]() ![]() | 116.1 MB 116.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8k4oMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.85 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_36888_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36888_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Recepto...
Entire | Name: Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Receptor (KSHV-GPCR)in complex with CXC chemokine CXCL1 and Gi protein |
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Components |
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-Supramolecule #1: Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Recepto...
Supramolecule | Name: Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Receptor (KSHV-GPCR)in complex with CXC chemokine CXCL1 and Gi protein type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: G protein-coupled receptor
Macromolecule | Name: G protein-coupled receptor / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 37.881773 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: DFLTIFLDDD ESWNETLNMS GYDYSGNFSL EVSVCEMTTV VPYTWNVGIL SLIFLINVLG NGLVTYIFCK HRSRAGAIDI LLLGICLNS LCLSISLLAE VLMFLFPNII STGLCRLEIF FYYLYVYLDI FSVVCVSLVR YLLVAYSTRS WPKKQSLGWV L TSAALLIA ...String: DFLTIFLDDD ESWNETLNMS GYDYSGNFSL EVSVCEMTTV VPYTWNVGIL SLIFLINVLG NGLVTYIFCK HRSRAGAIDI LLLGICLNS LCLSISLLAE VLMFLFPNII STGLCRLEIF FYYLYVYLDI FSVVCVSLVR YLLVAYSTRS WPKKQSLGWV L TSAALLIA LVLSGDACRH RSRVVDPVSK QAMCYENAGN MTADWRLHVR TVSVTAGFLL PLALLILFYA LTWCVVRRTK LQ ARRKVRG VIVAVVLLFF VFCFPYHVLN LLDTLLRRRW IRDSCYTRGL INVGLAVTSL LQALYSAVVP LIYSCLGSLF RQR MYGLFQ SLRQSFM UniProtKB: G protein-coupled receptor |
-Macromolecule #2: Growth-regulated alpha protein
Macromolecule | Name: Growth-regulated alpha protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 6.727939 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KII UniProtKB: Growth-regulated alpha protein |
-Macromolecule #3: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Macromolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 36.956383 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: DQLRQEAEQL KNQIRDARKA CADATLSQIT NNIDPVGRIQ MRTRRTLRGH LAKIYAMHWG TDSRLLVSAS QDGKLIIWDS YTTNKVHAI PLRSSWVMTC AYAPSGNYVA CGGLDNICSI YNLKTREGNV RVSRELAGHT GYLSCCRFLD DNQIVTSSGD T TCALWDIE ...String: DQLRQEAEQL KNQIRDARKA CADATLSQIT NNIDPVGRIQ MRTRRTLRGH LAKIYAMHWG TDSRLLVSAS QDGKLIIWDS YTTNKVHAI PLRSSWVMTC AYAPSGNYVA CGGLDNICSI YNLKTREGNV RVSRELAGHT GYLSCCRFLD DNQIVTSSGD T TCALWDIE TGQQTTTFTG HTGDVMSLSL APDTRLFVSG ACDASAKLWD VREGMCRQTF TGHESDINAI CFFPNGNAFA TG SDDATCR LFDLRADQEL MTYSHDNIIC GITSVSFSKS GRLLLAGYDD FNCNVWDALK ADRAGVLAGH DNRVSCLGVT DDG MAVATG SWDSFLKIWN UniProtKB: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
-Macromolecule #4: Guanine nucleotide-binding protein G(I) subunit alpha-1
Macromolecule | Name: Guanine nucleotide-binding protein G(I) subunit alpha-1 type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 39.914406 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: LSAEDKAAVE RSKMIDRNLR EDGEKAAREV KLLLLGAGES GKSTIVKQMK IIHEAGYSEE ECKQYKAVVY SNTIQSIIAI IRAMGRLKI DFGDAARADD ARQLFVLAGS AEEGFMTAEL AGVIKRLWKD GGVQACFSRS REYQLNDSAA YYLNDLDRIS Q GSYIPTQQ ...String: LSAEDKAAVE RSKMIDRNLR EDGEKAAREV KLLLLGAGES GKSTIVKQMK IIHEAGYSEE ECKQYKAVVY SNTIQSIIAI IRAMGRLKI DFGDAARADD ARQLFVLAGS AEEGFMTAEL AGVIKRLWKD GGVQACFSRS REYQLNDSAA YYLNDLDRIS Q GSYIPTQQ DVLRTRVKTT GIVETHFTFK DLHFKMFDVG GQRSERKKWI HCFEGVTAII FCVALSDYDL VLAEDEEMNR MH ESMKLFD SICNNKWFTD TSIILFLNKK DLFEEKIKKS PLTICYPEYA GSNTYEEAAA YIQCQFEDLN KRKDTKEIYT HFT CATDTK NVQFVFDAVT DVIIKNNLKD CGLF UniProtKB: Guanine nucleotide-binding protein G(i) subunit alpha-1 |
-Macromolecule #5: Guanine nucleotide-binding protein subunit gamma
Macromolecule | Name: Guanine nucleotide-binding protein subunit gamma / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 6.00197 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: IAQARKLVEQ LKMEANIDRI KVSKAAADLM AYCEAHAKED PLLTPVPASE NPFR UniProtKB: Guanine nucleotide-binding protein subunit gamma |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Grid | Material: GOLD |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.01 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 107180 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |