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- EMDB-36795: mycobacterial efflux pump, apo state -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-36795
Titlemycobacterial efflux pump, apo state
Map dataEM map of mycobacterial efflux pump, apo state
Sample
  • Complex: ternary complex of an ABC transporter Rv1217c-1218c
    • Protein or peptide: Multidrug efflux system permease protein Rv1217c
    • Protein or peptide: Multidrug efflux system ATP-binding protein Rv1218c
  • Ligand: (1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate
  • Ligand: CARDIOLIPIN
KeywordsABC transporter / efflux pump / TRANSPORT PROTEIN
Function / homology
Function and homology information


Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / response to antibiotic / ATP hydrolysis activity / ATP binding / plasma membrane
Similarity search - Function
Domain of unknown function DUF4162 / Domain of unknown function (DUF4162) / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Multidrug efflux system permease protein Rv1217c / Multidrug efflux system ATP-binding protein Rv1218c
Similarity search - Component
Biological speciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsWang Y / Wu F / Zhang L / Rao Z
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32170703 China
CitationJournal: To Be Published
Title: mycobacterial efflux pump, apo state
Authors: Wang Y / Wu F / Zhang L / Rao Z
History
DepositionJul 11, 2023-
Header (metadata) releaseJul 17, 2024-
Map releaseJul 17, 2024-
UpdateJul 17, 2024-
Current statusJul 17, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_36795.png

Downloads & links

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Map

FileDownload / File: emd_36795.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationEM map of mycobacterial efflux pump, apo state
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 384 pix.
= 314.88 Å		0.82 Å/pix.
x 384 pix.
= 314.88 Å		0.82 Å/pix.
x 384 pix.
= 314.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.82 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.5
Minimum - Maximum-1.891472 - 3.1224866
Average (Standard dev.)-0.00092182937 (±0.06413552)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 314.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map A of mycobacterial efflux pump, apo state

Fileemd_36795_half_map_1.map
Annotationhalf map A of mycobacterial efflux pump, apo state
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map B of mycobacterial efflux pump, apo state

Fileemd_36795_half_map_2.map
Annotationhalf map B of mycobacterial efflux pump, apo state
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : ternary complex of an ABC transporter Rv1217c-1218c

EntireName: ternary complex of an ABC transporter Rv1217c-1218c
Components
  • Complex: ternary complex of an ABC transporter Rv1217c-1218c
    • Protein or peptide: Multidrug efflux system permease protein Rv1217c
    • Protein or peptide: Multidrug efflux system ATP-binding protein Rv1218c
  • Ligand: (1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate
  • Ligand: CARDIOLIPIN

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Supramolecule #1: ternary complex of an ABC transporter Rv1217c-1218c

SupramoleculeName: ternary complex of an ABC transporter Rv1217c-1218c / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Molecular weightTheoretical: 120 KDa

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Macromolecule #1: Multidrug efflux system permease protein Rv1217c

MacromoleculeName: Multidrug efflux system permease protein Rv1217c / type: protein_or_peptide / ID: 1 / Details: transmembrane domain / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Molecular weightTheoretical: 56.698039 KDa
Recombinant expressionOrganism: Mycolicibacterium smegmatis MC2 155 (bacteria)
SequenceString: MSSTVIDRAR PAGHRAPHRG SGFTGTLGLL RLYLRRDRVS LPLWVLLLSV PLATVYIASV ETVYPDRSAR AAAAAAIMAS PAQRALYGP VYNDSLGAVG IWKAGMFHTL IAVAVILTVI RHTRADEESG RAELIDSTVV GRYANLTGAL LLSFGASIAT G AIGALGLL ...String:
MSSTVIDRAR PAGHRAPHRG SGFTGTLGLL RLYLRRDRVS LPLWVLLLSV PLATVYIASV ETVYPDRSAR AAAAAAIMAS PAQRALYGP VYNDSLGAVG IWKAGMFHTL IAVAVILTVI RHTRADEESG RAELIDSTVV GRYANLTGAL LLSFGASIAT G AIGALGLL ATDVAPAGSV AFGVALAASG MVFTAVAAVA AQLSPSARFT RAVAFAVLGT AFALRAIGDA GSGTLSWCSP LG WSLQVRP YAGERWWVLL LSLATAAVLT VLAYRLRAGR DVGAGLIAER PGAGTAGPML SEPFGLAWRL NRGSLLLWTV GLC LYGLVM GSVVHGIGDQ LGDNTAVRDI VTRMGGTGAL EQAFLALAFT MIGMVAAAFA VSLTLRLHQE ETGLRAETLL AGAV SRTHW LASHLAMALA GSAVATLISG VAAGLAYGMT VGDVGGKLPT VVGTAAVQLP AVWLLSAVTV GLFGLAPRFT PVAWG VLVG FIALYLLGSL AGFPQMLLNL EPFAHIPRVG GGDFTAVPLL WLLAIDAALI TLGAMAFRRR DVRC

UniProtKB: Multidrug efflux system permease protein Rv1217c

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Macromolecule #2: Multidrug efflux system ATP-binding protein Rv1218c

MacromoleculeName: Multidrug efflux system ATP-binding protein Rv1218c / type: protein_or_peptide / ID: 2 / Details: nucleotide binding domain / Number of copies: 2 / Enantiomer: LEVO
EC number: Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Molecular weightTheoretical: 31.227666 KDa
Recombinant expressionOrganism: Mycolicibacterium smegmatis MC2 155 (bacteria)
SequenceString: VPIEIRGLTK HFGSVRALDG LDLTVREGEV HGFLGPNGAG KSTTLRILLG LVKADGGSVR LLGGDPWTDA VDLHRHIAYV PGDVTLWPS LTGGETIDLL ARMRGGIDNA RRAELIERFG LDPTKKARTY SKGNRQKVSL ISALSSHATL LLLDEPSSGL D PLMENVFQ ...String:
VPIEIRGLTK HFGSVRALDG LDLTVREGEV HGFLGPNGAG KSTTLRILLG LVKADGGSVR LLGGDPWTDA VDLHRHIAYV PGDVTLWPS LTGGETIDLL ARMRGGIDNA RRAELIERFG LDPTKKARTY SKGNRQKVSL ISALSSHATL LLLDEPSSGL D PLMENVFQ QCIGEARQRG VTVLLSSHIL AETEALCEKV TIIRAGKTVE SGSLDALRHL SRTSIKAEMI GDPGDLSQIK GV EDISIEG TTVRAQVDSE SLRELIQVLG HAGVRSLVSQ PPTLEELFLR HY

UniProtKB: Multidrug efflux system ATP-binding protein Rv1218c

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Macromolecule #3: (1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadeca...

MacromoleculeName: (1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate
type: ligand / ID: 3 / Number of copies: 1 / Formula: L9Q
Molecular weightTheoretical: 746.05 Da
Chemical component information

ChemComp-L9Q:
(1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate

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Macromolecule #4: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 4 / Number of copies: 1 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8 mg/mL
BufferpH: 7.4 / Details: 150mM NaCl, 20mM Tris, detergent
GridMaterial: COPPER / Mesh: 300 / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK II
Detailssample was monodisperse

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 5900 / Average exposure time: 2.4 sec. / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 29000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1616966
Startup modelType of model: NONE
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 270648
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 4 / Software - Name: cryoSPARC

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