+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36708 | |||||||||
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Title | Cryo-EM structure of BlCHR2 class one | |||||||||
Map data | ||||||||||
Sample |
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Keywords | channelrhodopsin / MEMBRANE PROTEIN | |||||||||
Biological species | Bilabrum (eukaryote) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
Authors | Zhang MF | |||||||||
Funding support | 1 items
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Citation | Journal: To Be Published Title: Structure of BlCHR2 class one Authors: Zhang MF | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36708.map.gz | 943.7 MB | EMDB map data format | |
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Header (meta data) | emd-36708-v30.xml emd-36708.xml | 12.9 KB 12.9 KB | Display Display | EMDB header |
Images | emd_36708.png | 33.8 KB | ||
Filedesc metadata | emd-36708.cif.gz | 5 KB | ||
Others | emd_36708_half_map_1.map.gz emd_36708_half_map_2.map.gz | 929.1 MB 929.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36708 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36708 | HTTPS FTP |
-Validation report
Summary document | emd_36708_validation.pdf.gz | 1.2 MB | Display | EMDB validaton report |
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Full document | emd_36708_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | emd_36708_validation.xml.gz | 22 KB | Display | |
Data in CIF | emd_36708_validation.cif.gz | 25.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36708 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36708 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_36708.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.35 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_36708_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36708_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : BlCHR2
Entire | Name: BlCHR2 |
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Components |
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-Supramolecule #1: BlCHR2
Supramolecule | Name: BlCHR2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Bilabrum (eukaryote) |
-Macromolecule #1: BlCHR2
Macromolecule | Name: BlCHR2 / type: protein_or_peptide / ID: 1 / Details: GB:OP710242 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Bilabrum (eukaryote) |
Molecular weight | Theoretical: 43.984391 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MSECCELCVC QKEPGTFGAL IAVNTITAII LVAAGAYMAW KTAAGLGWNT RPHGPEGPPE ENWLSPGISI LCGVMYAFKA IDWASYNDT GESTAFSLNQ VWYSDYLITC PLLVLDFCIT VNLRYKLVFS SSIACLLAIA VSTFIVDAPY RYYMYGIGLA G FICAGYAL ...String: MSECCELCVC QKEPGTFGAL IAVNTITAII LVAAGAYMAW KTAAGLGWNT RPHGPEGPPE ENWLSPGISI LCGVMYAFKA IDWASYNDT GESTAFSLNQ VWYSDYLITC PLLVLDFCIT VNLRYKLVFS SSIACLLAIA VSTFIVDAPY RYYMYGIGLA G FICAGYAL WNEINAQREK IPDSAWWYLS AGRLIFFAGW PFFPLLWTLS FHTSGVINEE WYFILHAILD ILCKAVFGFF ML GFRLELE ELDFKAIEAE QAKLEGDKEA QALKNDKDTG EVLRHYNRAS SGFFGQGIPD DQGSVSGGSV LMSRARRQHM LYR REASYL SMDPMAEKIR ELEMLKKKIQ KEVDSRSKKM QREMTARFAI QDDSDDEDGG RGAGSARRRK NARRG |
-Macromolecule #2: POTASSIUM ION
Macromolecule | Name: POTASSIUM ION / type: ligand / ID: 2 / Number of copies: 18 / Formula: K |
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Molecular weight | Theoretical: 39.098 Da |
-Macromolecule #3: RETINAL
Macromolecule | Name: RETINAL / type: ligand / ID: 3 / Number of copies: 3 / Formula: RET |
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Molecular weight | Theoretical: 284.436 Da |
Chemical component information | ChemComp-RET: |
-Macromolecule #4: water
Macromolecule | Name: water / type: ligand / ID: 4 / Number of copies: 9 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 40700 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |