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- EMDB-35793: Middle segment of the bacteriophage M13 mini variant -

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Basic information

Entry
Database: EMDB / ID: EMD-35793
TitleMiddle segment of the bacteriophage M13 mini variant
Map data
Sample
  • Complex: middle segment of the bacteriophage M13 mini variant
    • Protein or peptide: Capsid protein G8P
KeywordsViral coat protein / M13 / VIRAL PROTEIN
Function / homologyPhage major coat protein, Gp8 / Bacteriophage M13, G8P, capsid domain superfamily / Capsid protein G8P / helical viral capsid / host cell plasma membrane / membrane / Capsid protein G8P
Function and homology information
Biological speciesInovirus M13
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsXiang Y / Jia Q
Funding support China, 2 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China) China
National Natural Science Foundation of China (NSFC) China
CitationJournal: Nat Commun / Year: 2023
Title: Cryo-EM structure of a bacteriophage M13 mini variant.
Authors: Qi Jia / Ye Xiang /
Abstract: Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM ...Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.
History
DepositionApr 1, 2023-
Header (metadata) releaseAug 16, 2023-
Map releaseAug 16, 2023-
UpdateSep 20, 2023-
Current statusSep 20, 2023Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_35793.png

Downloads & links

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Map

FileDownload / File: emd_35793.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.97 Å/pix.
x 240 pix.
= 232.8 Å		0.97 Å/pix.
x 240 pix.
= 232.8 Å		0.97 Å/pix.
x 240 pix.
= 232.8 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.97 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0142
Minimum - Maximum-0.049012493 - 0.09426401
Average (Standard dev.)0.00033819632 (±0.003727276)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 232.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_35793_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_35793_half_map_2.map
Projections & Slices
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Histogram

Histogram (log scale)

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Sample components

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Entire : middle segment of the bacteriophage M13 mini variant

EntireName: middle segment of the bacteriophage M13 mini variant
Components
  • Complex: middle segment of the bacteriophage M13 mini variant
    • Protein or peptide: Capsid protein G8P

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Supramolecule #1: middle segment of the bacteriophage M13 mini variant

SupramoleculeName: middle segment of the bacteriophage M13 mini variant / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Inovirus M13

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Macromolecule #1: Capsid protein G8P

MacromoleculeName: Capsid protein G8P / type: protein_or_peptide / ID: 1 / Number of copies: 40 / Enantiomer: LEVO
Source (natural)Organism: Inovirus M13
Molecular weightTheoretical: 5.243014 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
AEGDDPAKAA FNSLQASATE YIGYAWAMVV VIVGATIGIK LFKKFTSKAS

UniProtKB: Capsid protein G8P

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.7 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 368282
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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