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- EMDB-35635: Wheat 40S ribosome in complex with a tRNAi -

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Basic information

Entry
Database: EMDB / ID: EMD-35635
TitleWheat 40S ribosome in complex with a tRNAi
Map data
Sample
  • Complex: Wheat 40S ribosome in complex with a tRNAi
    • RNA: x 3 types
    • Protein or peptide: x 32 types
  • Ligand: x 2 types
Keywordsribosome / TRANSLATION
Function / homology
Function and homology information


endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / small-subunit processome / cytosolic ribosome / protein kinase C binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly ...endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / small-subunit processome / cytosolic ribosome / protein kinase C binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / ribosome binding / small ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / metal ion binding / nucleus / cytosol
Similarity search - Function
Ribosomal protein L41 / Ribosomal protein L41 / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e signature. ...Ribosomal protein L41 / Ribosomal protein L41 / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e signature. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / S25 ribosomal protein / Ribosomal protein S21e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S30 / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S11, N-terminal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / Ribosomal protein S4e, N-terminal, conserved site / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S19e signature. / Ribosomal S17 / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S17, archaeal/eukaryotic / 40S Ribosomal protein S10 / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S3Ae / Plectin/S10, N-terminal / Ribosomal S3Ae family / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal protein S7e signature. / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein S4e signature. / Ribosomal protein S8e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S6e signature. / Ribosomal protein S28e signature. / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein S14/S29 / Ribosomal protein S2 signature 2. / Ribosomal protein S3, conserved site / : / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / KH domain / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein uS8c / 40S ribosomal protein S4 / 40S ribosomal protein S6 / 40S ribosomal protein S17 / 40S ribosomal protein S8 / 40S ribosomal protein S16 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S29 / S5 DRBM domain-containing protein / 40S ribosomal protein S26 ...Small ribosomal subunit protein uS8c / 40S ribosomal protein S4 / 40S ribosomal protein S6 / 40S ribosomal protein S17 / 40S ribosomal protein S8 / 40S ribosomal protein S16 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S29 / S5 DRBM domain-containing protein / 40S ribosomal protein S26 / 40S ribosomal protein S24 / 40S ribosomal protein S30 / 30S ribosomal protein S11, chloroplastic / Small ribosomal subunit protein eS32 / 40S ribosomal protein S7 / Cytoplasmatic ribosomal protein S13 / S28 ribosomal protein / 30S ribosomal protein S3, chloroplastic / Putative ribosomal protein S18 / Ribosomal protein S20 / Uncharacterized protein / Plectin/eS10 N-terminal domain-containing protein / Small ribosomal subunit protein uS17 N-terminal domain-containing protein / Small ribosomal subunit protein uS7c / Uncharacterized protein / Small ribosomal subunit protein uS2 / 40S ribosomal protein S21 / 40S ribosomal protein S25 / 30S ribosomal protein S4, chloroplastic / 40S ribosomal protein S23 / 40S ribosomal protein S19 / 40S ribosomal protein S27
Similarity search - Component
Biological speciesTriticum aestivum (bread wheat) / Oryza sativa (Asian cultivated rice)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsYokoyama T / Tanaka M / Saito H / Nishimoto M / Tsuda K / Sotta N / Shigematsu H / Shirouzu M / Iwasaki S / Ito T / Fujiwara T
Funding support Japan, 1 items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)JP19H05637 Japan
CitationJournal: Nat Chem Biol / Year: 2024
Title: Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.
Authors: Mayuki Tanaka / Takeshi Yokoyama / Hironori Saito / Madoka Nishimoto / Kengo Tsuda / Naoyuki Sotta / Hideki Shigematsu / Mikako Shirouzu / Shintaro Iwasaki / Takuhiro Ito / Toru Fujiwara /
Abstract: In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is ...In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is downregulated in response to an excess amount of boric acid in the environment through upstream open reading frames (uORFs) that consist of only AUG and stop codons. However, the molecular details of how this minimum uORF controls translation of the downstream main ORF in a boric acid-dependent manner have remained unclear. Here, by combining ribosome profiling, translation complex profile sequencing, structural analysis with cryo-electron microscopy and biochemical assays, we show that the 80S ribosome assembled at AUG-stop migrates into the subsequent RNA segment, followed by downstream translation initiation, and that boric acid impedes this process by the stable confinement of eukaryotic release factor 1 on the 80S ribosome on AUG-stop. Our results provide molecular insight into translation regulation by a minimum and environment-responsive uORF.
History
DepositionMar 14, 2023-
Header (metadata) releaseFeb 21, 2024-
Map releaseFeb 21, 2024-
UpdateMay 15, 2024-
Current statusMay 15, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_35635.png

Downloads & links

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Map

FileDownload / File: emd_35635.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.788 Å
Density
Contour LevelBy AUTHOR: 0.013
Minimum - Maximum-0.027144127 - 0.06789487
Average (Standard dev.)-0.000036778714 (±0.0023960774)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 441.28 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_35635_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_35635_half_map_2.map
Projections & Slices
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Histogram

Histogram (log scale)

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Sample components

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Entire : Wheat 40S ribosome in complex with a tRNAi

EntireName: Wheat 40S ribosome in complex with a tRNAi
Components
  • Complex: Wheat 40S ribosome in complex with a tRNAi
    • RNA: 18S ribosomal RNA
    • Protein or peptide: 40S ribosomal protein eS24
    • Protein or peptide: 40S ribosomal protein eS8
    • Protein or peptide: 40S ribosomal protein eS10
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: RACK1Receptor for activated C kinase 1
    • Protein or peptide: 40S ribosomal protein uS3
    • Protein or peptide: 40S ribosomal protein eS4
    • Protein or peptide: 40S ribosomal protein uS7
    • Protein or peptide: 40S ribosomal protein uS9
    • Protein or peptide: 40S ribosomal protein uS10
    • Protein or peptide: 40S ribosomal protein uS11
    • Protein or peptide: 40S ribosomal protein uS13
    • Protein or peptide: 40S ribosomal protein uS15
    • Protein or peptide: 40S ribosomal protein eS17
    • Protein or peptide: 40S ribosomal protein eS19
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: 40S ribosomal protein eS25
    • Protein or peptide: 40S ribosomal protein eS28
    • Protein or peptide: 40S ribosomal protein uS8
    • Protein or peptide: 40S ribosomal protein uS14
    • Protein or peptide: 40S ribosomal protein eS27
    • Protein or peptide: 40S ribosomal protein eS30
    • Protein or peptide: 40S ribosomal protein uS2
    • Protein or peptide: 40S ribosomal protein eS1
    • Protein or peptide: 40S ribosomal protein eS21
    • Protein or peptide: 40S ribosomal protein eS26
    • Protein or peptide: 40S ribosomal protein uS4
    • Protein or peptide: 40S ribosomal protein uS5
    • Protein or peptide: 40S ribosomal protein eS6
    • Protein or peptide: 40S ribosomal protein eS7
    • Protein or peptide: 40S ribosomal protein uS17
    • Protein or peptide: 60S ribosomal protein eL41
    • RNA: mRNAMessenger RNA
    • RNA: tRNAi
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Wheat 40S ribosome in complex with a tRNAi

SupramoleculeName: Wheat 40S ribosome in complex with a tRNAi / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
Source (natural)Organism: Triticum aestivum (bread wheat)

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Macromolecule #1: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 583.613562 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUGC AAGUAUGAAC CAAUUUGAAC UGUGAAACU GCGAAUGGCU CAUUAAAUCA GUUAUAGUUU GUUUGAUGGU ACGUGCUACU CGGAUAACCG UAGUAAUUCU A GAGCUAAU ...String:
UACCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUGC AAGUAUGAAC CAAUUUGAAC UGUGAAACU GCGAAUGGCU CAUUAAAUCA GUUAUAGUUU GUUUGAUGGU ACGUGCUACU CGGAUAACCG UAGUAAUUCU A GAGCUAAU ACGUGCAACA AACCCCGACU UCUGGGAGGG GCGCAUUUAU UAGAUAAAAG GCUGACGCGG GCUCUGCUCG CU GAUCCGA UGAUUCAUGA UAACUCGACG GAUCGCACGG CCUUCGUGCC GGCGACGCAU CAUUCAAAUU UCUGCCCUAU CAA CUUUCG AUGGUAGGAU AGGGGCCUAC CAUGGUGGUG ACGGGUGACG GAGAAUUAGG GUUCGAUUCC GGAGAGGGAG CCUG AGAAA CGGCUACCAC AUCCAAGGAA GGCAGCAGGC GCGCAAAUUA CCCAAUCCUG ACACGGGGAG GUAGUGACAA UAAAU AACA AUACCGGGCG CAUUAGUGUC UGGUAAUUGG AAUGAGUACA AUCUAAAUCC CUUAACGAGG AUCCAUUGGA GGGCAA GUC UGGUGCCAGC AGCCGCGGUA AUUCCAGCUC CAAUAGCGUA UAUUUAAGUU GUUGCAGUUA AAAAGCUCGU AGUUGGA CC UUGGGCCGGG UCGGCCGGUC CGCCUCACGG CGAGCACCGA CCUACUCGAC CCUUCGGCCG GCAUCGCGCU CCUAGCCU U AAUUGGCCGG GUCGUGUUUC CGGCAUCGUU ACUUUGAAGA AAUUAGAGUG CUCAAAGCAA GCCAUCGCUC UGGAUACAU UAGCAUGGGA UAACAUCAUA GGAUUCCGGU CCUAUUGUGU UGGCCUUCGG GAUCGGAGUA AUGAUUAAUA GGGACAGUCG GGGGCAUUC GUAUUUCAUA GUCAGAGGUG AAAUUCUUGG AUUUAUGAAA GACGAACAAC UGCGAAAGCA UUUGCCAAGG A UGUUUUCA UUAAUCAAGA ACGAAAGUUG GGGGCUCGAA GACGAUCAGA UACCGUCCUA GUCUCAACCA UAAACGAUGC CG ACCAGGG AUCGGCGGAU GUUGCUUAUA GGACUCCGCC GGCACCUUAU GAGAAAUCAA AGUCUUUGGG UUCCGGGGGG AGU AUGGUC GCAAGGCUGA AACUUAAAGG AAUUGACGGA AGGGCACCAC CAGGCGUGGA GCCUGCGCUU AAUUUGACUC AACA CGGGG AAACUUACCA GGUCCAGACA UAGCAAGGAU UGACAGACUG AGAGCUCUUU CUUGAUUCUA UGGGUGGUGG UGCAU GGCC GUUCUUAGUU GGUGGAGCGA UUUGUCUGGU UAAUUCCGUU AACGAACGAG ACCUCAGCCU GCUAACUAGC UAUGCG GAG CCAUCCCUCC GCAGCUAGCU UCUUAGAGGG ACUAUGGCCG UUUAGGCCAC GGAAGUUUGA GGCAAUAACA GGUCUGU CA UGCCCUUAGA UGUUCUGGGC CGCACGCGCG CUACACUGAU GUAUUCAACG AGUAUAUAGC CUUGGCCGAC AGGCCCGG G UAAUCUUGGG AAAUUUCAUC GUGAUGGGGA UAGAUCAUUG CAAUUGUUGG UCUUCAACGA GGAAUGCCUA GUAAGCGCG AGUCAUCAGC UCGCGUUGAC UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUCCUAC CGAUUGAAUG GUCCGGUGAA GUGUUCGGA UCGCGGCGAC GGGGGCGGUU CGCCGCCCCC GACGUCGCGA GAAGUCCAUU GAACCUUAUC AUUUAGAGGA A GCAGAAGU CGUAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UG

GENBANK: GENBANK: XR_006476226.1

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Macromolecule #34: mRNA

MacromoleculeName: mRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 2.229403 KDa
SequenceString:
AUGUAAA

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Macromolecule #35: tRNAi

MacromoleculeName: tRNAi / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 24.446926 KDa
SequenceString:
AUCAGAGU(1MG)(2MG) CGCAGCGGAA GCGU(2MG)G(PSU)GGG CCCAU(MIA)ACCC ACAG(G7M)(H2U)(5MC) (5MC)C AGGA(PSU)CG(1MA)AA CCUGGCUCUG AUACCA

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Macromolecule #2: 40S ribosomal protein eS24

MacromoleculeName: 40S ribosomal protein eS24 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.739685 KDa
SequenceString:
MADAKTAPAV TLRTRKFMTN RLLSRKQFVL EVIHPGRANV SKADLKERLA KIYEVKDSNC IFVFKFRTHF GGGKSTGFGL IYDNLEAAK KFEPKYRLIR NGLATKVEKS RKQIKERKNR TKKIRGVKKT KAGDAKKK

UniProtKB: 40S ribosomal protein S24

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Macromolecule #3: 40S ribosomal protein eS8

MacromoleculeName: 40S ribosomal protein eS8 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 25.341096 KDa
SequenceString: MGISRDSMHK RRATGGKQKA WRKKRKYELG RQPANTKLSS NKTVRRVRVR GGNVKWRALR LDTGNFSWGS EAVTRKTRLL DVVYNSSNN ELVRTQTLVK NAIVQVDAAP FKQWYLTHYG VEIGRKKKAA AAAKKEPAEG QEAEVAAAAT EEAKKSNNVQ R KLEKRQQG ...String:
MGISRDSMHK RRATGGKQKA WRKKRKYELG RQPANTKLSS NKTVRRVRVR GGNVKWRALR LDTGNFSWGS EAVTRKTRLL DVVYNSSNN ELVRTQTLVK NAIVQVDAAP FKQWYLTHYG VEIGRKKKAA AAAKKEPAEG QEAEVAAAAT EEAKKSNNVQ R KLEKRQQG RTLDSHIEEQ FSGGRLLACI SSRPGQCGRA DGYILEGKEL EFYMRKLQKK KGKGATA

UniProtKB: 40S ribosomal protein S8

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Macromolecule #4: 40S ribosomal protein eS10

MacromoleculeName: 40S ribosomal protein eS10 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 20.463965 KDa
SequenceString:
MIISKKNRNE ICKYLFQEGV LYAKKDYNLA KHPQIDVPNL QVIKLMQSFK SKEYVRETFS WQHYYWYLTN DGIEFLRNFL NLPSEIVPA TLKKSARPPG RPLGSGPPGD RPRGPPRFEG DRPRYGDRDG YRGGPRGAPG DFGGEKGGAP AEFQPSFRSS G GGGFGRGG GGGFGRGGGG GGGFGASPME

UniProtKB: Plectin/eS10 N-terminal domain-containing protein

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Macromolecule #5: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.696476 KDa
SequenceString:
MGKTRGMGAG RKLKTHRRNQ RWADKAYKKS HLGNEWKKPF AGSSHAKGIV LEKIGIEAKQ PNSAIRKCAR VQLVKNGKKI AAFVPNDGC LNFIEENDEV LIAGFGRKGH AVGDIPGVRF KVVKVSGVSL LALFKEKKEK PRS

UniProtKB: 40S ribosomal protein S23

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Macromolecule #6: RACK1

MacromoleculeName: RACK1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 36.200527 KDa
SequenceString: MAGAQESLVY AGVMRGHNDV VTAIATPIDN SPFIVSSSRD KSLLVWDLTN PIQATQDSSS EYGVPFRRLT GHGHFVQDVV LSSDGQFAL SGSWDGELRL WDLSTGVTTR RFVGHEKDVL SVAFSIDNRQ IVSASRDRTI KLWNTLGECK YTIGGDLGGG E GHTGWVSC ...String:
MAGAQESLVY AGVMRGHNDV VTAIATPIDN SPFIVSSSRD KSLLVWDLTN PIQATQDSSS EYGVPFRRLT GHGHFVQDVV LSSDGQFAL SGSWDGELRL WDLSTGVTTR RFVGHEKDVL SVAFSIDNRQ IVSASRDRTI KLWNTLGECK YTIGGDLGGG E GHTGWVSC VRFSPNNFAP TIVSGSWDRS VKVWNLTNCK LRCTLDGHGG YVSAVAVSPD GSLCASGGKD GVTLLWDLTE GK RLYSLDA GSIINSLCFS PNRYWLCAAT QDSIKIWDLE SKHIVQDLRP EVPVSTKQML YCTCLSWSAD GSTLYAGYTD GTI RIYKIS GFSYSS

UniProtKB: Uncharacterized protein

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Macromolecule #7: 40S ribosomal protein uS3

MacromoleculeName: 40S ribosomal protein uS3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 25.413787 KDa
SequenceString: MATQISKKKK FVSDGVFYAE LNEMLTRELA EDGYSGVEVR VTPMRTEIII RATRTQNVLG EKGRRIRELT SVVQKRFNFL ENGVELYAE KVVNRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFVMES GAKGCEVIVS GKLRAQRAKS MKFKDGYMIS S GQPVNEYI ...String:
MATQISKKKK FVSDGVFYAE LNEMLTRELA EDGYSGVEVR VTPMRTEIII RATRTQNVLG EKGRRIRELT SVVQKRFNFL ENGVELYAE KVVNRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFVMES GAKGCEVIVS GKLRAQRAKS MKFKDGYMIS S GQPVNEYI DAAVRHVLLR QGVLGIKVKI MLDWDPKGKL GPTTPLPDLV TIHPPKEENE LRPPALVEV

UniProtKB: 30S ribosomal protein S3, chloroplastic

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Macromolecule #8: 40S ribosomal protein eS4

MacromoleculeName: 40S ribosomal protein eS4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 30.008111 KDa
SequenceString: MARGLKKHLK RLNAPSHWML DKLGGAFAPK PSSGPHKARE CLPLILILRN RLKYALTYRE VQSILMQRHI MVDGKVRTDK TYPAGFMDI ISIPKTGENY RLLYDTKGRF RLHSVRDEDA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLETNK ...String:
MARGLKKHLK RLNAPSHWML DKLGGAFAPK PSSGPHKARE CLPLILILRN RLKYALTYRE VQSILMQRHI MVDGKVRTDK TYPAGFMDI ISIPKTGENY RLLYDTKGRF RLHSVRDEDA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLETNK IVDFIKFDVG NVVMVTGGRN TGRVGVIKNR EKHKGTFETI HVEDAQGHQF ATRLGNVFTI GKGTKPWVSL PK GKGIKLT IIEEQRKRDA AAQAAAKA

UniProtKB: 40S ribosomal protein S4

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Macromolecule #9: 40S ribosomal protein uS7

MacromoleculeName: 40S ribosomal protein uS7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.350879 KDa
SequenceString: MAVVEPVPGG DVKLFNRWTF EDVQVSDISL NDYLAVTSMK HYTFLPHSAG RYSAKRFRKA QCPIIERLTN SLMMHGRNNG KKIMAVRII KHTMEIIHLL TDANPIQIIV DAIINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL LTTGARESAF R NVKTIAEC ...String:
MAVVEPVPGG DVKLFNRWTF EDVQVSDISL NDYLAVTSMK HYTFLPHSAG RYSAKRFRKA QCPIIERLTN SLMMHGRNNG KKIMAVRII KHTMEIIHLL TDANPIQIIV DAIINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL LTTGARESAF R NVKTIAEC LADELINAAK GSSNSYAIKK KDEIERVAKA NR

UniProtKB: Small ribosomal subunit protein uS7c

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Macromolecule #10: 40S ribosomal protein uS9

MacromoleculeName: 40S ribosomal protein uS9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.870992 KDa
SequenceString:
MAAVLTRPTP GTVQCFGRKK TAVAVAYCKP GRGLIKVNGA PIELIRPEML RLKAFEPILL AGRSRFKDID MRIRVRGGGK TSQIYSIRQ AIAKSLVAYY QKYVDEAAKK EVKEIFARYD RTLLVADPRR CEPKKFGGRG ARARFQKSYR

UniProtKB: 40S ribosomal protein S16

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Macromolecule #11: 40S ribosomal protein uS10

MacromoleculeName: 40S ribosomal protein uS10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.128448 KDa
SequenceString:
MATELAYAPP MKSGKLGFEG TQEVQHRIRI TLSSKSVKNL EKVCSDLVKG AKEKQLKVKG PVRMPTKVLN ITTRKSPCGE GTNTWDRFE MRVHKRVIDL VSSPDVVKQI TSITIEPGVE VEVTISDQ

UniProtKB: Ribosomal protein S20

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Macromolecule #12: 40S ribosomal protein uS11

MacromoleculeName: 40S ribosomal protein uS11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.398818 KDa
SequenceString:
MSSKRKTREP KEENVTLGPA VREGEHVFGV AHIFASFNDT FIHVTDLSGR ETLVRITGGM KVKADRDESS PYAAMLASQD VATRCKELG ITALHIKLRA TGGNKTKTPG PGAQSALRAL ARSGMKIGRI EDVTPVPTDS TRRKGGRRGR RL

UniProtKB: 30S ribosomal protein S11, chloroplastic

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Macromolecule #13: 40S ribosomal protein uS13

MacromoleculeName: 40S ribosomal protein uS13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.702686 KDa
SequenceString:
MSLIAGEEFQ HILRVLNTNV DGKQKIMFAL TSIKGVGRRF SNIVCKKADI DMNKRAGELS AEEMDRLMAV VHNPRQFKVP DWFLNRKKD YKDGRFSQVV SNAVDMKLRD DLERLKKIRN HRGLRHYWGV RVRGQHTKTT GRRGKTVGVS KKR

UniProtKB: Putative ribosomal protein S18

+
Macromolecule #14: 40S ribosomal protein uS15

MacromoleculeName: 40S ribosomal protein uS15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.102277 KDa
SequenceString:
MGRMHSRGKG ISSSALPYKR TPPSWVKTAV ADVDELITKA AKKGQMPSQI GVLLRDQHGI PLVKSVTGSK ILRILKAHGL APEIPEDLY FLIKKAVAIR KHLERNRKDK DSKFRLILVE SRIHRLARYY KRTKKLPPTW KYESATASTL VA

UniProtKB: Cytoplasmatic ribosomal protein S13

+
Macromolecule #15: 40S ribosomal protein eS17

MacromoleculeName: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.523143 KDa
SequenceString:
MGRVRTKTVK KTSRQVIEKY YSRMTLDFHT NKKVLEEVSI LQSKRLRNKV AGFTTHLMRR IQRGPVRGIS LKLQEEERER RMDFVPEKS ALEVDEIRVD KETMDMLAFL GMADLPGVER APEVTSSAAP YRQPFNGPRG GNRA

UniProtKB: 40S ribosomal protein S17

+
Macromolecule #16: 40S ribosomal protein eS19

MacromoleculeName: 40S ribosomal protein eS19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.097604 KDa
SequenceString:
MATVPAPAAE TAASTARTVK DVNPHEFVKA YSAHLKRSGK MELPEWVDIV KTARFKELPP SDADWYYIRA ASMARKIYLR QGIGIGGFQ KIYGGRQRNG SRPPHFCKSS GAVSRNILQE LQKMGIIDVD PKGGRLITSQ GRRDLDQVAG TVPAEF

UniProtKB: 40S ribosomal protein S19

+
Macromolecule #17: 40S ribosomal protein uS19

MacromoleculeName: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.424557 KDa
SequenceString:
MADVDVDTEV AAGAQPKKRT FRKFSYRGVD LDALLDMSTD DLVQMFPARA RRRFKRGLKR KPMALVKKLR KAKKDAPAGE KPEPVRTHL RNMIIVPEMI GSLIGVYNGK TFNQVEIKPE MIGHYLAEFS ISYKPVKHGR PGIGATHSSR FIPLK

UniProtKB: Uncharacterized protein

+
Macromolecule #18: 40S ribosomal protein eS25

MacromoleculeName: 40S ribosomal protein eS25 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 12.094188 KDa
SequenceString:
MAPKKEKAPA ASSKPAKSGG GKQKKKKWSK GKQKEKVNNA VLFDQATYDK LLTEVPKYKQ ITPSVLSERL RINGSLARRA IKDLMERGL IRMVSIHSSQ QIFTRATNT

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #19: 40S ribosomal protein eS28

MacromoleculeName: 40S ribosomal protein eS28 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 9.815345 KDa
SequenceString:
RGRLHRCSRS SDPPSPTPQE SMDTQVKLAV VVKVMGRTGS RGQVTQVRVK FLDDQNRLIM RNVKGPVREG DILTLLESER EARRLR

UniProtKB: S28 ribosomal protein

+
Macromolecule #20: 40S ribosomal protein uS8

MacromoleculeName: 40S ribosomal protein uS8 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.924326 KDa
SequenceString:
MGRRILNDAL RTMVNAERRG KATAQLQPIS GVMISFLNIM KHRGYIKNFE VFDPHRVGKI TVELQGRIKD CKALTYRQDL RATEIEKYR TRMLPTRQWG YVVVTTPNGV LDHEEAIRQN VGGQVLGYFH

UniProtKB: Small ribosomal subunit protein uS8c

+
Macromolecule #21: 40S ribosomal protein uS14

MacromoleculeName: 40S ribosomal protein uS14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.391455 KDa
SequenceString:
MGHSNVWNSH PKNYGAGSRV CRVCGNSHGL IRKYGLMCCR QCFRSNAKDI GFIKYR

UniProtKB: 40S ribosomal protein S29

+
Macromolecule #22: 40S ribosomal protein eS27

MacromoleculeName: 40S ribosomal protein eS27 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 9.583195 KDa
SequenceString:
MVLQNDIDLL NPPAELEKLK HKKKRLVQSP NSFFMDVKCQ GCFNITTVFS HSQTVVVCPG CQTVLCQPTG GKARLTEGCS FRRKGD

UniProtKB: 40S ribosomal protein S27

+
Macromolecule #23: 40S ribosomal protein eS30

MacromoleculeName: 40S ribosomal protein eS30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.934164 KDa
SequenceString:
MGKVHGSLAR AGKVRGQTPK VAKQDKKKQP RGRAHKRIQY NRRFVTAVVG FGKKRGPNSS EK

UniProtKB: 40S ribosomal protein S30

+
Macromolecule #24: 40S ribosomal protein uS2

MacromoleculeName: 40S ribosomal protein uS2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 33.300438 KDa
SequenceString: MAAAAGEAPR ALSQKEQDIQ MMLAADVHLG TKNCDFQMER YAYKRRSDGI YIINLGKTWE KLQLAARVIV AIENPQDIIV QSARPYGQR AVLKFAQHTG ANAIAGRHTP GTFTNQMQTS FSEPRLLILT DPRTDHQPIK ESALGNIPTI AFCDTDSPMR Y VDIGIPAN ...String:
MAAAAGEAPR ALSQKEQDIQ MMLAADVHLG TKNCDFQMER YAYKRRSDGI YIINLGKTWE KLQLAARVIV AIENPQDIIV QSARPYGQR AVLKFAQHTG ANAIAGRHTP GTFTNQMQTS FSEPRLLILT DPRTDHQPIK ESALGNIPTI AFCDTDSPMR Y VDIGIPAN NKGRNSIGCL YWLLARMVLQ MRGTILPGHK WDVMVDLFFY RDPEEAKEQE DEAAGAPEYA AITDYGAAAG QW GGDQWTS DAPAPPPVTG GEWPMAEAPV AGGDGWDAAG APVAVEGAVP APVVAATGWD AAVQPPAQGW E

UniProtKB: Small ribosomal subunit protein uS2

+
Macromolecule #25: 40S ribosomal protein eS1

MacromoleculeName: 40S ribosomal protein eS1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 30.043955 KDa
SequenceString: MAVGKNKRIS KGRKGSKKKA VDPFTKKQWY DIKAPLLFTS RNVGKTLVSR TQGTKIASEG LKHRVFEVSL ADLQNDEDQA YRKIRLRAE DVQGMNVLTN FWGMDFTTDK LRSLVRKWQT LIEAHVDVKT TDNYMLRMFA IGFTKRRPNQ VKRTCYAQAS Q IRQIRRKM ...String:
MAVGKNKRIS KGRKGSKKKA VDPFTKKQWY DIKAPLLFTS RNVGKTLVSR TQGTKIASEG LKHRVFEVSL ADLQNDEDQA YRKIRLRAE DVQGMNVLTN FWGMDFTTDK LRSLVRKWQT LIEAHVDVKT TDNYMLRMFA IGFTKRRPNQ VKRTCYAQAS Q IRQIRRKM VEIMVNQAAS CDLKELVNKF IPEVIGKEIE KATSSIFPLQ NVYVRKVKIL KAPKFDLGKL MEVHGDYKED VG VKLDRPA DGDEVIPGAE EVAAAE

UniProtKB: Small ribosomal subunit protein eS1

+
Macromolecule #26: 40S ribosomal protein eS21

MacromoleculeName: 40S ribosomal protein eS21 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 9.194349 KDa
SequenceString:
MQNEVGDMVD LYVPRKCSAT NRIITAKDHA SVQINIGHVD ENGLYDGRFT TFALSGFVRA QGDADGSLDR LWQKRKAEIK QL

UniProtKB: 40S ribosomal protein S21

+
Macromolecule #27: 40S ribosomal protein eS26

MacromoleculeName: 40S ribosomal protein eS26 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.576164 KDa
SequenceString:
MTFKRRNGGR NKHGRGHVKY IRCSNCAKCC PKDKAIKRFL VRNIVEQAAV RDVQEACVHD GYVLPKLYAK VHHCVSCAIH AHIVRVRSR ENRRNREPPQ RFRRRDDGPR PGQGGPPRAG GPGAPGGAPA GAGAVPGAPG GAPAGAGAVP GAPAPRV

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #28: 40S ribosomal protein uS4

MacromoleculeName: 40S ribosomal protein uS4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.534111 KDa
SequenceString: MVHVNFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARH LLTLDEKNPR RIFEGAALLR RMNRYGLLA EGQDKLDYVL ALTAENFLAR RLQTLVFKAG MAKSIHHARV LIRQRHIRVG RQIVNVPSFM VRVESEKHID F SLTSPFGG ...String:
MVHVNFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARH LLTLDEKNPR RIFEGAALLR RMNRYGLLA EGQDKLDYVL ALTAENFLAR RLQTLVFKAG MAKSIHHARV LIRQRHIRVG RQIVNVPSFM VRVESEKHID F SLTSPFGG GPAGRVKRKN QKKASGGGDA GGDEEEE

UniProtKB: 30S ribosomal protein S4, chloroplastic

+
Macromolecule #29: 40S ribosomal protein uS5

MacromoleculeName: 40S ribosomal protein uS5 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 29.995975 KDa
SequenceString: MADRGGERGV DRGGFGRGFG RGGRGDRGGR RGGRRGPRQE EEKWVPVTKL GRLVKEGRFT KMEEIYLHSL PVKEHQIVET LCPGLKDEV MKITPVQKQT RAGQRTRFKA FVVVGDSNGH VGLGVKCAKE VATAIRGAII LAKLSIVPVR RGYWGNKIGL P HTVPCKVT ...String:
MADRGGERGV DRGGFGRGFG RGGRGDRGGR RGGRRGPRQE EEKWVPVTKL GRLVKEGRFT KMEEIYLHSL PVKEHQIVET LCPGLKDEV MKITPVQKQT RAGQRTRFKA FVVVGDSNGH VGLGVKCAKE VATAIRGAII LAKLSIVPVR RGYWGNKIGL P HTVPCKVT GKCGSVTVRM VPAPRGSGIV AARVPKKVLQ FAGIDDVFTS SRGSTKTLGN FVKATFDCLM KTYGFLTPDF WR ETTFTKA PYQEFTDILA KPTKALMLDA PAEKVEA

UniProtKB: S5 DRBM domain-containing protein

+
Macromolecule #30: 40S ribosomal protein eS6

MacromoleculeName: 40S ribosomal protein eS6 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 28.488574 KDa
SequenceString: MKLNIANPTT GCQKKVEIDD DMKLRNLYDK RISQEVVGDL LGEEFEGYIF KIMGGCDKQG FPMKQGVLTP GRVRLLLHRG TSCFRGHGR RVGERRRKSV RGCIVSQDLS VINLVIVKKG KNDLPGLTDT EKPRMRGPKR ASKIKKLFNL GKDDDVRQYV N TYRRSFTN ...String:
MKLNIANPTT GCQKKVEIDD DMKLRNLYDK RISQEVVGDL LGEEFEGYIF KIMGGCDKQG FPMKQGVLTP GRVRLLLHRG TSCFRGHGR RVGERRRKSV RGCIVSQDLS VINLVIVKKG KNDLPGLTDT EKPRMRGPKR ASKIKKLFNL GKDDDVRQYV N TYRRSFTN KKGKTVSKAP KIQRLVTPLT LQRKRARIAD KKKRIAKKKS EAAEYQKLLA QRLKEQRDRR SESMAKRRSK LS AATKAPA ASA

UniProtKB: 40S ribosomal protein S6

+
Macromolecule #31: 40S ribosomal protein eS7

MacromoleculeName: 40S ribosomal protein eS7 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.297891 KDa
SequenceString: MYTARKKIQK DKGVEPSEFE DTVAQAFFDL ENGNQELKSD LKDLYINTAI QMDVVGNRKA VVIHVPYRLR KPFRKIHVRL VRELEKKFS GKDVVFVATR RIVRPPKKGS AVQRPRTRTL TAVHDGILED VVYPAEIVGK RVRYRLDGAK VIKIYLDPKE R NNTEYKLE ...String:
MYTARKKIQK DKGVEPSEFE DTVAQAFFDL ENGNQELKSD LKDLYINTAI QMDVVGNRKA VVIHVPYRLR KPFRKIHVRL VRELEKKFS GKDVVFVATR RIVRPPKKGS AVQRPRTRTL TAVHDGILED VVYPAEIVGK RVRYRLDGAK VIKIYLDPKE R NNTEYKLE TFSAVYRRLC GKDVVFEYPV TETA

UniProtKB: 40S ribosomal protein S7

+
Macromolecule #32: 40S ribosomal protein uS17

MacromoleculeName: 40S ribosomal protein uS17 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.841061 KDa
SequenceString:
MAEQTEKSFL KQPKVFLSTK KADKAKRPGK AGNRFWKSVG LGFKTPREAI DGTYIDKKCP FTGTVAIRGR IIAGTCHSAK MNRTIIVRR NYLHFVKKYQ RYEKRHSNIP AHISPCFRVK EGDHVIIGQC RPLSKTVRFN VLKVVPAGTT GGGKKAFIAA

UniProtKB: Small ribosomal subunit protein uS17 N-terminal domain-containing protein

+
Macromolecule #33: 60S ribosomal protein eL41

MacromoleculeName: 60S ribosomal protein eL41 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryza sativa (Asian cultivated rice)
Molecular weightTheoretical: 3.445437 KDa
SequenceString:
MRAKWKKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

+
Macromolecule #36: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 84 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #37: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 37 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeJEOL CRYO ARM 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 60000
Sample stageSpecimen holder model: JEOL CRYOSPECPORTER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2

-
Image processing

Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 41361
FSC plot (resolution estimation)

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