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Yorodumi- EMDB-33522: The pre-fusion structure of Thogotovirus dhori envelope glycoprotein -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-33522 | |||||||||
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Title | The pre-fusion structure of Thogotovirus dhori envelope glycoprotein | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Viral surface protein / STRUCTURAL PROTEIN | |||||||||
Function / homology | Baculovirus Gp64, envelope glycoprotein / Baculovirus gp64 envelope glycoprotein family / modulation by virus of host process / viral envelope / virion membrane / membrane / Envelope glycoprotein Function and homology information | |||||||||
Biological species | Dhori thogotovirus | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
Authors | Shan YY / Zhang MF / Pei DQ | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Pre- and post-fusion class III glycoprotein structures reveal viral fusion mechanism Authors: Shan YY / Zhang MF / Pei DQ | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_33522.map.gz | 398.3 MB | EMDB map data format | |
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Header (meta data) | emd-33522-v30.xml emd-33522.xml | 12.5 KB 12.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_33522_fsc.xml | 15.8 KB | Display | FSC data file |
Images | emd_33522.png | 33.8 KB | ||
Others | emd_33522_half_map_1.map.gz emd_33522_half_map_2.map.gz | 391.9 MB 391.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-33522 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-33522 | HTTPS FTP |
-Related structure data
Related structure data | 7xymMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_33522.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.849 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_33522_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_33522_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Thogotovirus dhori envelope glycoprotein
Entire | Name: Thogotovirus dhori envelope glycoprotein |
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Components |
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-Supramolecule #1: Thogotovirus dhori envelope glycoprotein
Supramolecule | Name: Thogotovirus dhori envelope glycoprotein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Dhori thogotovirus |
-Macromolecule #1: Envelope glycoprotein
Macromolecule | Name: Envelope glycoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Dhori thogotovirus / Strain: Indian/1313/61 |
Molecular weight | Theoretical: 58.674496 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MDSTIRLVAT IFLISLTQQI EVCNKAQQQG PYTLVDYQEK PLNISRIQIK VVKTSVATKG LNFHIGYRAV WRSYCYNGGS LDKNTGCYN DLIPKSPTES ELRTWSKSQK CCTGPDAVDA WGSDARICWA EWKMELCHTA KELKKYSNNN HFAYHTCNLS W RCGLKSTH ...String: MDSTIRLVAT IFLISLTQQI EVCNKAQQQG PYTLVDYQEK PLNISRIQIK VVKTSVATKG LNFHIGYRAV WRSYCYNGGS LDKNTGCYN DLIPKSPTES ELRTWSKSQK CCTGPDAVDA WGSDARICWA EWKMELCHTA KELKKYSNNN HFAYHTCNLS W RCGLKSTH IEVRLQASGG LVSMVAVMPN GTLIPIEGTR PTYWTEDSFA YLYDPAGTEK KTESTFLWCF KEHIRPTTEL SG AVYDTHY LGGTYDKNPQ FNYYCRDNGY YFELPANRLV CLPTSCYKRE GAIVNTMHPD TWKVSEKLHS ASQFDVNNVV HSL VYETEG LRLALSQLDH RFATLSRLFN RLTQSLAKID DRLLGTLLGQ DVSSKFISPT KFMLSPCLST PEGDSNCHNH SIYR DGRWV HNSDPTQCFS LSKSQPVDLY SFKELWLPQL LDVNVEGVVA DEEGWSFVAQ SKQALIDTMT YTKNGGKGTS LEDVL GYPS GWINGKLQGL LLNGAISWVV VIGVVLVGVC LMRRVF UniProtKB: Envelope glycoprotein |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.5 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |