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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | AhCS-3Mg2+-FSPP | |||||||||
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![]() | (-)-cyperene synthase / directed evolution / Alphafold2 / PLANT PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Yu SS / Zhu P / Liu YB / Ma SG / Ye D / Shao YZ / Li WR / Cui ZJ | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Characterisation and engineering of two highly paralogous sesquiterpene synthases reveal a regioselective reprotonation switch. Authors: Ye D / Shao YZ / Li WR / Cui ZJ / Gong T / Yang JL / Wang HQ / Dai JG / Feng KP / Ma M / Ma SG / Liu YB / Zhu P / Yu SS | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.5 KB 9.5 KB | Display Display | ![]() |
Images | ![]() | 84.3 KB | ||
Filedesc metadata | ![]() | 4.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 489.4 KB | Display | ![]() |
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Full document | ![]() | 488.9 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Data in CIF | ![]() | 6.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7xkwM M: atomic model generated by this map |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.81 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : AhCS-3Mg2+-FSPP
Entire | Name: AhCS-3Mg2+-FSPP |
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Components |
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-Supramolecule #1: AhCS-3Mg2+-FSPP
Supramolecule | Name: AhCS-3Mg2+-FSPP / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: (-)-cyperene synthase
Macromolecule | Name: (-)-cyperene synthase / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MGSSHHHHHH SSGLVPRGSH MMGRSSANFH PNVWGDRFVM MPSKEMETDA STKQRAEMLK QEVKKMLHDV SGSLQELNLI NEIQRLGVAY HFEAEISNAL ERIYNREKNE NGINDCDLHA VALRFRLLRQ HGYNVSSDVF KKFKDENGEF EARFRNDVRG LLSLYEAAYF ...String: MGSSHHHHHH SSGLVPRGSH MMGRSSANFH PNVWGDRFVM MPSKEMETDA STKQRAEMLK QEVKKMLHDV SGSLQELNLI NEIQRLGVAY HFEAEISNAL ERIYNREKNE NGINDCDLHA VALRFRLLRQ HGYNVSSDVF KKFKDENGEF EARFRNDVRG LLSLYEAAYF GTQEDGHLDE AIAFTTKHLK SLLPHLSSPL SDLVKLALDL PLLKRIERLQ SKHFISIYQQ DEDRNNVLLE FAKIDFNILQ ALHRKELNEI TRWWNESDLP RKLPFIRDRL VECYIWMLEL YYEPQYSQSR RMTTILLILT SSMDDIYDVY GKLEELERYT VAVERWEREA LDQLPDYMRV HLGVLLTVVE NFEDELGKEG KSFHLAYVKK AFAELTKAYI KEARWANAEH VPALEEYVDN AVVSSAYPLI TTMSLLGMGK VATKEAFEWT ISLPNAVRQC SMICRLVNDI MSNQSEQERV HVASAVQCCM KEFSTTYEEA CKILQEKIAR AWKDLNKECL HPIPVSLELI RRPLNLARAI EFYYQCRDGY SDSAFEIKDY ITMLLVEPAV V |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 283635 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |