+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-29522 | |||||||||
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Title | Asp-bound GltPh RSMR mutant in IFS-A4 state | |||||||||
Map data | Asp-bound GltPh RSMR mutant in IFS-A4 state | |||||||||
Sample |
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Biological species | Pyrococcus horikoshii (archaea) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.85 Å | |||||||||
Authors | Huang Y / Boudker O | |||||||||
Funding support | United States, 2 items
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Citation | Journal: J.Am.Chem.Soc. / Year: 2023 Title: Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM Authors: Huang Y / Reddy KD / Bracken C / Qiu B / Zhan W / Eliezer D / Boudker O | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_29522.map.gz | 83.5 MB | EMDB map data format | |
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Header (meta data) | emd-29522-v30.xml emd-29522.xml | 12.4 KB 12.4 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_29522_fsc.xml | 11.6 KB | Display | FSC data file |
Images | emd_29522.png | 105 KB | ||
Others | emd_29522_half_map_1.map.gz emd_29522_half_map_2.map.gz | 154.6 MB 154.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-29522 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-29522 | HTTPS FTP |
-Validation report
Summary document | emd_29522_validation.pdf.gz | 992.5 KB | Display | EMDB validaton report |
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Full document | emd_29522_full_validation.pdf.gz | 992.1 KB | Display | |
Data in XML | emd_29522_validation.xml.gz | 19.6 KB | Display | |
Data in CIF | emd_29522_validation.cif.gz | 24.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-29522 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-29522 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_29522.map.gz / Format: CCP4 / Size: 166.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Asp-bound GltPh RSMR mutant in IFS-A4 state | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.852 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map 1
File | emd_29522_half_map_1.map | ||||||||||||
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Annotation | Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 2
File | emd_29522_half_map_2.map | ||||||||||||
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Annotation | Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : GltPh
Entire | Name: GltPh |
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Components |
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-Supramolecule #1: GltPh
Supramolecule | Name: GltPh / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Pyrococcus horikoshii (archaea) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 4.5 mg/mL |
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Buffer | pH: 7.4 |
Vitrification | Cryogen name: ETHANE / Chamber temperature: 303 K |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.94 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 1.3 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Refinement | Protocol: FLEXIBLE FIT |
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