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- EMDB-29028: CryoEM structure of Conalbumin from chicken egg white (sigma-Cas ... -
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Open data
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Basic information
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Title | CryoEM structure of Conalbumin from chicken egg white (sigma-Cas 1391-06-6) | |||||||||
![]() | Final map | |||||||||
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Function / homology | ![]() extracellular sequestering of iron ion / organomineral extracellular matrix / antimicrobial humoral response / intracellular sequestering of iron ion / ferric iron binding / acute-phase response / recycling endosome / iron ion transport / antibacterial humoral response / response to lipopolysaccharide ...extracellular sequestering of iron ion / organomineral extracellular matrix / antimicrobial humoral response / intracellular sequestering of iron ion / ferric iron binding / acute-phase response / recycling endosome / iron ion transport / antibacterial humoral response / response to lipopolysaccharide / early endosome / response to xenobiotic stimulus / iron ion binding / extracellular space / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.0 Å | |||||||||
![]() | Newton TP / Zimanyi C / Kopylov M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CryoEM structure of Conalbumin from chicken egg white (sigma-Cas 1391-06-6) Authors: Newton TP / Zimanyi C / Kopylov M | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 61.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.9 KB 14.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.6 KB | Display | ![]() |
Images | ![]() | 74.3 KB | ||
Masks | ![]() | 125 MB | ![]() | |
Others | ![]() ![]() | 116 MB 116 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8feiMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Final map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.674 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Final half map A
File | emd_29028_half_map_1.map | ||||||||||||
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Annotation | Final half map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Final half map B
File | emd_29028_half_map_2.map | ||||||||||||
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Annotation | Final half map B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Conalbumin from chicken egg white
Entire | Name: Conalbumin from chicken egg white |
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Components |
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-Supramolecule #1: Conalbumin from chicken egg white
Supramolecule | Name: Conalbumin from chicken egg white / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Ovotransferrin
Macromolecule | Name: Ovotransferrin / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 77.878531 KDa |
Sequence | String: MKLILCTVLS LGIAAVCFAA PPKSVIRWCT ISSPEEKKCN NLRDLTQQER ISLTCVQKAT YLDCIKAIAN NEADAISLDG GQAFEAGLA PYKLKPIAAE VYEHTEGSTT SYYAVAVVKK GTEFTVNDLQ GKTSCHTGLG RSAGWNIPIG TLLHRGAIEW E GIESGSVE ...String: MKLILCTVLS LGIAAVCFAA PPKSVIRWCT ISSPEEKKCN NLRDLTQQER ISLTCVQKAT YLDCIKAIAN NEADAISLDG GQAFEAGLA PYKLKPIAAE VYEHTEGSTT SYYAVAVVKK GTEFTVNDLQ GKTSCHTGLG RSAGWNIPIG TLLHRGAIEW E GIESGSVE QAVAKFFSAS CVPGATIEQK LCRQCKGDPK TKCARNAPYS GYSGAFHCLK DGKGDVAFVK HTTVNENAPD QK DEYELLC LDGSRQPVDN YKTCNWARVA AHAVVARDDN KVEDIWSFLS KAQSDFGVDT KSDFHLFGPP GKKDPVLKDL LFK DSAIML KRVPSLMDSQ LYLGFEYYSA IQSMRKDQLT PSPRENRIQW CAVGKDEKSK CDRWSVVSNG DVECTVVDET KDCI IKIMK GEADAVALDG GLVYTAGVCG LVPVMAERYD DESQCSKTDE RPASYFAVAV ARKDSNVNWN NLKGKKSCHT AVGRT AGWV IPMGLIHNRT GTCNFDEYFS EGCAPGSPPN SRLCQLCQGS GGIPPEKCVA SSHEKYFGYT GALRCLVEKG DVAFIQ HST VEENTGGKNK ADWAKNLQMD DFELLCTDGR RANVMDYREC NLAEVPTHAV VVRPEKANKI RDLLERQEKR FGVNGSE KS KFMMFESQNK DLLFKDLTKC LFKVREGTTY KEFLGDKFYT VISSLKTCNP SDILQMCSFL EGK |
-Macromolecule #2: FE (III) ION
Macromolecule | Name: FE (III) ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: FE |
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Molecular weight | Theoretical: 55.845 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 / Details: PBS |
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Grid | Model: UltrAuFoil / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: OTHER |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 58.53 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.001 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |