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- EMDB-29027: CryoEM structure of bacteriophage Q-beta coat protein dimer with ... -
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Open data
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Basic information
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Title | CryoEM structure of bacteriophage Q-beta coat protein dimer with AYGG linker | |||||||||
![]() | Final map | |||||||||
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![]() | coat protein / q-beta / fusion / VIRUS LIKE PARTICLE | |||||||||
Function / homology | Read-through domain / Read-through domain / Levivirus coat protein / Levivirus coat protein / Bacteriophage RNA-type, capsid / viral capsid / structural molecule activity / Minor capsid protein A1![]() | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.28 Å | |||||||||
![]() | Newton TP / Zhao L / Finn MG / Kopylov M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CryoEM structure of bacteriophage Q-beta coat protein dimer with AYGG linker Authors: Newton TP / Zhao L / Kopylov M / Finn MG | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 255 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.8 KB 13.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 16.9 KB | Display | ![]() |
Images | ![]() | 142.6 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Filedesc metadata | ![]() | 5.2 KB | ||
Others | ![]() ![]() | 475.6 MB 475.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 26.8 KB | Display | |
Data in CIF | ![]() | 35.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8fehMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Final map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.855 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Final half map A
File | emd_29027_half_map_1.map | ||||||||||||
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Annotation | Final half map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Final half map B
File | emd_29027_half_map_2.map | ||||||||||||
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Annotation | Final half map B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Qubevirus durum
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Qubevirus durum
Supramolecule | Name: Qubevirus durum / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 39803 / Sci species name: Qubevirus durum / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: OTHER / Virus enveloped: No / Virus empty: Yes |
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-Macromolecule #1: Minor capsid protein A1 fusion
Macromolecule | Name: Minor capsid protein A1 fusion / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 28.604137 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: AKLETVTLGN IGKDGKQTLV LNPRGVNPTN GVASLSQAGA VPALEKRVTV SVSQPSRNRK NYKVQVKIQN PTACTANGSC DPSVTRQAY ADVTFSFTQY STDEERAFVR TELAALLASP LLIDAIDQLN PAYAYGGAKL ETVTLGNIGK DGKQTLVLNP R GVNPTNGV ...String: AKLETVTLGN IGKDGKQTLV LNPRGVNPTN GVASLSQAGA VPALEKRVTV SVSQPSRNRK NYKVQVKIQN PTACTANGSC DPSVTRQAY ADVTFSFTQY STDEERAFVR TELAALLASP LLIDAIDQLN PAYAYGGAKL ETVTLGNIGK DGKQTLVLNP R GVNPTNGV ASLSQAGAVP ALEKRVTVSV SQPSRNRKNY KVQVKIQNPT ACTANGSCDP SVTRQAYADV TFSFTQYSTD EE RAFVRTE LAALLASPLL IDAIDQLNPA Y UniProtKB: Minor capsid protein A1 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Average electron dose: 64.7 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.001 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |