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- EMDB-26471: Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO2) -

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Basic information

Entry
Database: EMDB / ID: EMD-26471
TitlePhotosynthetic assembly of Chlorobaculum tepidum (RC-FMO2)
Map dataRC-FMO2 map
Sample
  • Complex: Photosynthetic assembly (RC-FMO2)
    • Protein or peptide: x 7 types
  • Ligand: x 9 types
KeywordsFMO / reaction center / single-particle cryo-EM / bacteriochlorophyll / photosynthesis / electron transport chain / energy transfer
Function / homology
Function and homology information


thylakoid / bacteriochlorophyll binding / iron-sulfur cluster binding / photosynthesis / electron transfer activity / heme binding / membrane / metal ion binding / plasma membrane
Similarity search - Function
Proteolipid membrane potential modulator / Proteolipid membrane potential modulator / Photosystem P840 reaction-centre, cytochrome c-551 / Photosystem P840 reaction-centre cytochrome c-551 / Photosystem I P840 reaction centre protein PscD / Photosystem P840 reaction centre protein PscD / Bacteriochlorophyll A protein / Bacteriochlorophyll A superfamily / Bacteriochlorophyll A protein / Photosystem I PsaA/PsaB ...Proteolipid membrane potential modulator / Proteolipid membrane potential modulator / Photosystem P840 reaction-centre, cytochrome c-551 / Photosystem P840 reaction-centre cytochrome c-551 / Photosystem I P840 reaction centre protein PscD / Photosystem P840 reaction centre protein PscD / Bacteriochlorophyll A protein / Bacteriochlorophyll A superfamily / Bacteriochlorophyll A protein / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / 4Fe-4S dicluster domain / Cytochrome c-like domain superfamily / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
Cytochrome c / Bacteriochlorophyll a protein / Photosystem P840 reaction center, large subunit / Photosystem P840 reaction center iron-sulfur protein / Uncharacterized protein / P840 reaction center 17 kDa protein / Ric1 protein
Similarity search - Component
Biological speciesChlorobaculum tepidum TLS (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.08 Å
AuthorsPuskar R / Truong CD / Swain K / Li S / Cheng K-W / Wang TY / Poh Y-P / Liu H / Chou T-F / Nannenga B / Chiu P-L
Funding support United States, 2 items
OrganizationGrant numberCountry
Other governmentW911NF2010321
National Science Foundation (NSF, United States)NSF MRI 1531991 United States
CitationJournal: Nat Commun / Year: 2022
Title: Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria.
Authors: Ryan Puskar / Chloe Du Truong / Kyle Swain / Saborni Chowdhury / Ka-Yi Chan / Shan Li / Kai-Wen Cheng / Ting Yu Wang / Yu-Ping Poh / Yuval Mazor / Haijun Liu / Tsui-Fen Chou / Brent L Nannenga / Po-Lin Chiu /
Abstract: The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex ...The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex mediates the transfer of light energy from the chlorosome antenna complex to the RC. Here we determine the structure of the photosynthetic supercomplex from the GSB Chlorobaculum tepidum using single-particle cryogenic electron microscopy (cryo-EM) and identify the cytochrome c subunit (PscC), two accessory protein subunits (PscE and PscF), a second FMO trimeric complex, and a linker pigment between FMO and the RC core. The protein subunits that are assembled with the symmetric RC core generate an asymmetric photosynthetic supercomplex. One linker bacteriochlorophyll (BChl) is located in one of the two FMO-PscA interfaces, leading to differential efficiencies of the two energy transfer branches. The two FMO trimeric complexes establish two different binding interfaces with the RC cytoplasmic surface, driven by the associated accessory subunits. This structure of the GSB photosynthetic supercomplex provides mechanistic insight into the light excitation energy transfer routes and a possible evolutionary transition intermediate of the bacterial photosynthetic supercomplex from the primitive homodimeric RC.
History
DepositionMar 21, 2022-
Header (metadata) releaseOct 5, 2022-
Map releaseOct 5, 2022-
UpdateJun 12, 2024-
Current statusJun 12, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_26471.png

Downloads & links

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Map

FileDownload / File: emd_26471.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRC-FMO2 map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 360 pix.
= 374.4 Å		1.04 Å/pix.
x 360 pix.
= 374.4 Å		1.04 Å/pix.
x 360 pix.
= 374.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.04 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.428
Minimum - Maximum-2.408888 - 4.8352113
Average (Standard dev.)-0.000068901056 (±0.11211566)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 374.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: RC-FMO2 half map 1

Fileemd_26471_half_map_1.map
AnnotationRC-FMO2 half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: RC-FMO2 half map 2

Fileemd_26471_half_map_2.map
AnnotationRC-FMO2 half map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Photosynthetic assembly (RC-FMO2)

EntireName: Photosynthetic assembly (RC-FMO2)
Components
  • Complex: Photosynthetic assembly (RC-FMO2)
    • Protein or peptide: Photosystem P840 reaction center, large subunit
    • Protein or peptide: Photosystem P840 reaction center iron-sulfur protein
    • Protein or peptide: Cytochrome c
    • Protein or peptide: P840 reaction center 17 kDa protein
    • Protein or peptide: PscE
    • Protein or peptide: PscF
    • Protein or peptide: Bacteriochlorophyll a proteinBacteriochlorophyll
  • Ligand: Bacteriochlorophyll A isomer
  • Ligand: Chlorophyll A ester
  • Ligand: BACTERIOCHLOROPHYLL ABacteriochlorophyll
  • Ligand: [(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate
  • Ligand: 2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: CALCIUM IONCalcium

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Supramolecule #1: Photosynthetic assembly (RC-FMO2)

SupramoleculeName: Photosynthetic assembly (RC-FMO2) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#7
Details: Photosynthetic assembly from Chlorobaculum tepidum (RC-FMO2)
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Location in cell: Membrane
Molecular weightTheoretical: 504 KDa

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Macromolecule #1: Photosystem P840 reaction center, large subunit

MacromoleculeName: Photosystem P840 reaction center, large subunit / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 81.784641 KDa
SequenceString: MAEQVKPAGV KPKGTVPPPK GNAPAPKANG APGGASVIKE QDAAKMRRFL FQRTETRSTK WYQIFDTEKL DDEQVVGGHL ALLGVLGFI MGIYYISGIQ VFPWGAPGFH DNWFYLTIKP RMVSLGIDTY STKTADLEAA GARLLGWAAF HFLVGSVLIF G GWRHWTHN ...String:
MAEQVKPAGV KPKGTVPPPK GNAPAPKANG APGGASVIKE QDAAKMRRFL FQRTETRSTK WYQIFDTEKL DDEQVVGGHL ALLGVLGFI MGIYYISGIQ VFPWGAPGFH DNWFYLTIKP RMVSLGIDTY STKTADLEAA GARLLGWAAF HFLVGSVLIF G GWRHWTHN LTNPFTGRCG NFRDFRFLGK FGDVVFNGTS AKSYKEALGP HAVYMSLLFL GWGIVMWAIL GFAPIPDFQT IN SETFMSF VFAVIFFALG IYWWNNPPNA AIHLNDDMKA AFSVHLTAIG YINIALGCIA FVAFQQPSFA PYYKELDKLV FYL YGEPFN RVSFNFVEQG GKVISGAKEF ADFPAYAILP KSGEAFGMAR VVTNLIVFNH IICGVLYVFA GVYHGGQYLL KIQL NGMYN QIKSIWITKG RDQEVQVKIL GTVMALCFAT MLSVYAVIVW NTICELNIFG TNITMSFYWL KPLPIFQWMF ADPSI NDWV MAHVITAGSL FSLIALVRIA FFAHTSPLWD DLGLKKNSYS FPCLGPVYGG TCGVSIQDQL WFAMLWGIKG LSAVCW YID GAWIASMMYG VPAADAKAWD SIAHLHHHYT SGIFYYFWTE TVTIFSSSHL STILMIGHLV WFISFAVWFE DRGSRLE GA DIQTRTIRWL GKKFLNRDVN FRFPVLTISD SKLAGTFLYF GGTFMLVFLF LANGFYQTNS PLPPPVSHAA VSGQQMLA Q LVDTLMKMIA

UniProtKB: Photosystem P840 reaction center, large subunit

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Macromolecule #2: Photosystem P840 reaction center iron-sulfur protein

MacromoleculeName: Photosystem P840 reaction center iron-sulfur protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 23.540289 KDa
SequenceString: MAEPVENKNQ APAPGAKVPP KGAPAAPKAG APAAPKGPVA PKAGAPAAKT GASAAKQAGK PRLASLGVTL GRSGVRQESA LPYVKPKAV PPPKPAAPAA KGAPAPKGAP AAPAAKAAPG APVAKAAPKA KKHYFIIENL CVGCGLCLDK CPPKVNAIGY K FYGDVQEG ...String:
MAEPVENKNQ APAPGAKVPP KGAPAAPKAG APAAPKGPVA PKAGAPAAKT GASAAKQAGK PRLASLGVTL GRSGVRQESA LPYVKPKAV PPPKPAAPAA KGAPAPKGAP AAPAAKAAPG APVAKAAPKA KKHYFIIENL CVGCGLCLDK CPPKVNAIGY K FYGDVQEG GFRCYIDQAA CISCSACFSG DECPSGALIE VLPDGEVLDF SYTPPERLDF DLRFLHRFHR EAR

UniProtKB: Photosystem P840 reaction center iron-sulfur protein

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Macromolecule #3: Cytochrome c

MacromoleculeName: Cytochrome c / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 22.741779 KDa
SequenceString: MDKNSNGKLI ALAVGGAVLM GALFFSVSFL TGYIPAPNHS AILTPLRSFM GWFLLIFCAS IIIMGLGKMS SAISDKWFLS FPLSIFVIV MVMFLSLRVY WEKGRTTTVD GKYIRTTAEL KEFLNKPAAT SDVPPAPAGF DFDAAKKLVD VRCNKCHTLD S VADLFRTK ...String:
MDKNSNGKLI ALAVGGAVLM GALFFSVSFL TGYIPAPNHS AILTPLRSFM GWFLLIFCAS IIIMGLGKMS SAISDKWFLS FPLSIFVIV MVMFLSLRVY WEKGRTTTVD GKYIRTTAEL KEFLNKPAAT SDVPPAPAGF DFDAAKKLVD VRCNKCHTLD S VADLFRTK YKKTGQVNLI VKRMQGFPGS GISDDDAKTI GIWLHEKF

UniProtKB: Cytochrome c

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Macromolecule #4: P840 reaction center 17 kDa protein

MacromoleculeName: P840 reaction center 17 kDa protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 16.633195 KDa
SequenceString:
MQPQLSRPQT ASNQVRKAVS GPWSGNAVHK AEKYFITSAK RDRDGKLQIE LVPASGRRKL SPTPEMIRRL IDGEIEIYIL TTQPDIAID MNKEIIDMEN RYVIDFDKRG VKWTMREIPV FYHEGKGLCV ELHNKIYTLD QFFK

UniProtKB: P840 reaction center 17 kDa protein

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Macromolecule #5: PscE

MacromoleculeName: PscE / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 6.690926 KDa
SequenceString:
MDAKTTILEV LKKEGKPMSA GQIAEKSGLE RKEVDKAMKS LKEEELIVSP KRCYWTPKV

UniProtKB: Uncharacterized protein

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Macromolecule #6: PscF

MacromoleculeName: PscF / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 6.227533 KDa
SequenceString:
MDIRRLILAF ILPPAAVMNK EAGTIMLTGI LTLWGWIPGV VAALIMISKE QSGKTAEA

UniProtKB: Ric1 protein

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Macromolecule #7: Bacteriochlorophyll a protein

MacromoleculeName: Bacteriochlorophyll a protein / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Chlorobaculum tepidum TLS (bacteria) / Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
Molecular weightTheoretical: 40.34343 KDa
SequenceString: MALFGSNDVT TAHSDYEIVL EGGSSSWGKV KARAKVNAPP ASPLLPADCD VKLNVKPLDP AKGFVRISAV FESIVDSTKN KLTIEADIA NETKERRISV GEGMVSVGDF SHTFSFEGSV VNLFYYRSDA VRRNVPNPIY MQGRQFHDIL MKVPLDNNDL I DTWEGTVK ...String:
MALFGSNDVT TAHSDYEIVL EGGSSSWGKV KARAKVNAPP ASPLLPADCD VKLNVKPLDP AKGFVRISAV FESIVDSTKN KLTIEADIA NETKERRISV GEGMVSVGDF SHTFSFEGSV VNLFYYRSDA VRRNVPNPIY MQGRQFHDIL MKVPLDNNDL I DTWEGTVK AIGSTGAFND WIRDFWFIGP AFTALNEGGQ RISRIEVNGL NTESGPKGPV GVSRWRFSHG GSGMVDSISR WA ELFPSDK LNRPAQVEAG FRSDSQGIEV KVDGEFPGVS VDAGGGLRRI LNHPLIPLVH HGMVGKFNNF NVDAQLKVVL PKG YKIRYA APQYRSQNLE EYRWSGGAYA RWVEHVCKGG VGQFEILYAQ

UniProtKB: Bacteriochlorophyll a protein

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Macromolecule #8: Bacteriochlorophyll A isomer

MacromoleculeName: Bacteriochlorophyll A isomer / type: ligand / ID: 8 / Number of copies: 2 / Formula: GS0
Molecular weightTheoretical: 911.504 Da
Chemical component information

ChemComp-GS0:
Bacteriochlorophyll A isomer

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Macromolecule #9: Chlorophyll A ester

MacromoleculeName: Chlorophyll A ester / type: ligand / ID: 9 / Number of copies: 4 / Formula: G2O
Molecular weightTheoretical: 891.473 Da
Chemical component information

ChemComp-G2O:
Chlorophyll A ester

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Macromolecule #10: BACTERIOCHLOROPHYLL A

MacromoleculeName: BACTERIOCHLOROPHYLL A / type: ligand / ID: 10 / Number of copies: 73 / Formula: BCL
Molecular weightTheoretical: 911.504 Da
Chemical component information

ChemComp-BCL:
BACTERIOCHLOROPHYLL A / Bacteriochlorophyll

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Macromolecule #11: [(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-...

MacromoleculeName: [(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate
type: ligand / ID: 11 / Number of copies: 3 / Formula: F39
Molecular weightTheoretical: 895.299 Da
Chemical component information

ChemComp-F39:
[(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate

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Macromolecule #12: 2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethy...

MacromoleculeName: 2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene
type: ligand / ID: 12 / Number of copies: 3 / Formula: F26
Molecular weightTheoretical: 532.841 Da
Chemical component information

ChemComp-F26:
2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene

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Macromolecule #13: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 13 / Number of copies: 10 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #14: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 14 / Number of copies: 6 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #15: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 15 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #16: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 16 / Number of copies: 2 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
20.0 mMTris-ClTris-Cl
200.0 mMNaClSodium chlorideNaClSodium chloride
0.17 mMDDMDDM
GridModel: C-flat-2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
VitrificationCryogen name: ETHANE / Instrument: HOMEMADE PLUNGER

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated magnification: 47259 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 32898 / Average exposure time: 6.0 sec. / Average electron dose: 45.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1938908
Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final 3D classificationSoftware - Name: cryoSPARC (ver. 3.3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 3.08 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.1) / Number images used: 157486

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Atomic model buiding 1

Initial modelPDB ID:

6m32


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: OTHER / Overall B value: 44.07
Output model

PDB-7ueb:
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO2)

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