ジャーナル: Proc Natl Acad Sci U S A / 年: 2019 タイトル: Three-dimensional architecture of epithelial primary cilia. 著者: Shufeng Sun / Rebecca L Fisher / Samuel S Bowser / Brian T Pentecost / Haixin Sui / 要旨: We report a complete 3D structural model of typical epithelial primary cilia based on structural maps of full-length primary cilia obtained by serial section electron tomography. Our data demonstrate ...We report a complete 3D structural model of typical epithelial primary cilia based on structural maps of full-length primary cilia obtained by serial section electron tomography. Our data demonstrate the architecture of primary cilia differs extensively from the commonly acknowledged 9+0 paradigm. The axoneme structure is relatively stable but gradually evolves from base to tip with a decreasing number of microtubule complexes (MtCs) and a reducing diameter. The axonemal MtCs are cross-linked by previously unrecognized fibrous protein networks. Such an architecture explains why primary cilia can elastically withstand liquid flow for mechanosensing. The nine axonemal MtCs in a cilium are found to differ significantly in length indicating intraflagellar transport processes in primary cilia may be more complicated than that reported for motile cilia. The 3D maps of microtubule doublet-singlet transitions generally display longitudinal gaps at the inner junction between the A- and B-tubules, which indicates the inner junction protein is a major player in doublet-singlet transitions. In addition, vesicles releasing from kidney primary cilia were observed in the structural maps, supporting that ciliary vesicles budding may serve as ectosomes for cell-cell communication.
タイプ: POSITIVE / 材質: uranyl acetate, lead citrate 詳細: 2% uranyl acetate and Reynolds lead citrate solution
糖包埋
材質: epoxy resin 詳細: The samples were infiltrated with a graded series of resins (SPI-PON 812, SPI-CHEM, USA) in acetone over 24 hours. After infiltration with pure resin (3 x within 24 hours), the specimens in ...詳細: The samples were infiltrated with a graded series of resins (SPI-PON 812, SPI-CHEM, USA) in acetone over 24 hours. After infiltration with pure resin (3 x within 24 hours), the specimens in resin were polymerized at 60 degrees C for 2 days.