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- EMDB-19076: Cryo-EM structure of P. urativorans 70S ribosome in complex with ... -

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Basic information

Entry
Database: EMDB / ID: EMD-19076
TitleCryo-EM structure of P. urativorans 70S ribosome in complex with hibernation factor Balon, mRNA and P-site tRNA (structure 2).
Map data
Sample
  • Complex: Ribosomes isolated from ice-treated cultures of Psychrobacter urativorans.
    • Protein or peptide: x 51 types
    • RNA: x 4 types
  • RNA: x 1 types
KeywordsRibosome / Hibernation factor / Dormancy / Balon
Function / homology
Function and homology information


guanosine tetraphosphate binding / translation elongation factor activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity ...guanosine tetraphosphate binding / translation elongation factor activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / GTPase activity / mRNA binding / GTP binding / RNA binding / cytosol / cytoplasm
Similarity search - Function
Bacterial archaeo-eukaryotic release factor family 3 / Bacterial archaeo-eukaryotic release factor family 3 / Ribosomal protein L31 type B / Translation elongation factor EFTu/EF1A, C-terminal / Translation elongation factor EFTu/EF1A, bacterial/organelle / Elongation factor Tu, domain 2 / Elongation factor Tu (EF-Tu), GTP-binding domain / : / Elongation factor Tu C-terminal domain / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal ...Bacterial archaeo-eukaryotic release factor family 3 / Bacterial archaeo-eukaryotic release factor family 3 / Ribosomal protein L31 type B / Translation elongation factor EFTu/EF1A, C-terminal / Translation elongation factor EFTu/EF1A, bacterial/organelle / Elongation factor Tu, domain 2 / Elongation factor Tu (EF-Tu), GTP-binding domain / : / Elongation factor Tu C-terminal domain / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Elongation factor Tu domain 2 / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL2 / Methyl-accepting chemotaxis protein / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL30 ...Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL2 / Methyl-accepting chemotaxis protein / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL35 / Elongation factor Tu / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL4 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein bL21
Similarity search - Component
Biological speciesPsychrobacter urativorans (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsHelena-Bueno K / Rybak MY / Gagnon MG / Hill CH / Melnikov SV
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Royal SocietyRGS/R2/202003 United Kingdom
Citation
Journal: Nature / Year: 2024
Title: A new family of bacterial ribosome hibernation factors.
Authors: Karla Helena-Bueno / Mariia Yu Rybak / Chinenye L Ekemezie / Rudi Sullivan / Charlotte R Brown / Charlotte Dingwall / Arnaud Baslé / Claudia Schneider / James P R Connolly / James N Blaza / ...Authors: Karla Helena-Bueno / Mariia Yu Rybak / Chinenye L Ekemezie / Rudi Sullivan / Charlotte R Brown / Charlotte Dingwall / Arnaud Baslé / Claudia Schneider / James P R Connolly / James N Blaza / Bálint Csörgő / Patrick J Moynihan / Matthieu G Gagnon / Chris H Hill / Sergey V Melnikov /
Abstract: To conserve energy during starvation and stress, many organisms use hibernation factor proteins to inhibit protein synthesis and protect their ribosomes from damage. In bacteria, two families of ...To conserve energy during starvation and stress, many organisms use hibernation factor proteins to inhibit protein synthesis and protect their ribosomes from damage. In bacteria, two families of hibernation factors have been described, but the low conservation of these proteins and the huge diversity of species, habitats and environmental stressors have confounded their discovery. Here, by combining cryogenic electron microscopy, genetics and biochemistry, we identify Balon, a new hibernation factor in the cold-adapted bacterium Psychrobacter urativorans. We show that Balon is a distant homologue of the archaeo-eukaryotic translation factor aeRF1 and is found in 20% of representative bacteria. During cold shock or stationary phase, Balon occupies the ribosomal A site in both vacant and actively translating ribosomes in complex with EF-Tu, highlighting an unexpected role for EF-Tu in the cellular stress response. Unlike typical A-site substrates, Balon binds to ribosomes in an mRNA-independent manner, initiating a new mode of ribosome hibernation that can commence while ribosomes are still engaged in protein synthesis. Our work suggests that Balon-EF-Tu-regulated ribosome hibernation is a ubiquitous bacterial stress-response mechanism, and we demonstrate that putative Balon homologues in Mycobacteria bind to ribosomes in a similar fashion. This finding calls for a revision of the current model of ribosome hibernation inferred from common model organisms and holds numerous implications for how we understand and study ribosome hibernation.
#1: Journal: Biorxiv / Year: 2023
Title: A bacterial ribosome hibernation factor with evolutionary connections to eukaryotic protein synthesis
Authors: Helena-Bueno K / Ekemezie CL / Brown CR / Basle A / Blaza JN / Hill CH / Melnikov SV
History
DepositionDec 8, 2023-
Header (metadata) releaseFeb 21, 2024-
Map releaseFeb 21, 2024-
UpdateMar 13, 2024-
Current statusMar 13, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_19076.map.gz / Format: CCP4 / Size: 600.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.72 Å/pix.
x 540 pix.
= 390.42 Å
0.72 Å/pix.
x 540 pix.
= 390.42 Å
0.72 Å/pix.
x 540 pix.
= 390.42 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.723 Å
Density
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.089290306 - 0.18286471
Average (Standard dev.)0.00047362337 (±0.004765882)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions540540540
Spacing540540540
CellA=B=C: 390.41998 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_19076_msk_1.map
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Half map: #2

Fileemd_19076_half_map_1.map
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Half map: #1

Fileemd_19076_half_map_2.map
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Sample components

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Entire : Ribosomes isolated from ice-treated cultures of Psychrobacter ura...

EntireName: Ribosomes isolated from ice-treated cultures of Psychrobacter urativorans.
Components
  • Complex: Ribosomes isolated from ice-treated cultures of Psychrobacter urativorans.
    • Protein or peptide: Methyl-accepting chemotaxis protein
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Elongation factor Tu
    • RNA: 23S rRNA
    • Protein or peptide: Small ribosomal subunit protein bS18
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Large ribosomal subunit protein uL5
    • RNA: 5S rRNA
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein bL32
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL31B
    • Protein or peptide: Small ribosomal subunit protein uS14
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein bS21
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL14
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: Large ribosomal subunit protein bL19
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Large ribosomal subunit protein bL33
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Large ribosomal subunit protein bL36
    • RNA: Heterogenous tRNA
    • RNA: Heterogenous mRNA
    • Protein or peptide: Large ribosomal subunit protein bL9
  • RNA: 16S rRNA

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Supramolecule #1: Ribosomes isolated from ice-treated cultures of Psychrobacter ura...

SupramoleculeName: Ribosomes isolated from ice-treated cultures of Psychrobacter urativorans.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#55
Source (natural)Organism: Psychrobacter urativorans (bacteria)

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Macromolecule #1: Methyl-accepting chemotaxis protein

MacromoleculeName: Methyl-accepting chemotaxis protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 41.159938 KDa
SequenceString: MKATLKSLRE TKANPAVSIF VKTHREHPAN DQDPIALKNQ LKIVEERLTN EFDKRTATTI LDKIHAETKE YNHKYNLDTL AIFASVDDV QLIRMPLDTP ERVVISNRFA TRDLVRDMAS AVHYYTVVLT RDKARLIEAA NDRVVAEFDT DNAKQNNMEG I PFPIDNNG ...String:
MKATLKSLRE TKANPAVSIF VKTHREHPAN DQDPIALKNQ LKIVEERLTN EFDKRTATTI LDKIHAETKE YNHKYNLDTL AIFASVDDV QLIRMPLDTP ERVVISNRFA TRDLVRDMAS AVHYYTVVLT RDKARLIEAA NDRVVAEFDT DNAKQNNMEG I PFPIDNNG LYTTGDGGSD RSANNENSYL KEFFNQVDKS VQELWGEHKM PLVIVGDVRN ISYYKEVCDR PENIIATVSN LT ELEDGNA QRIIDGVQDA VEGYRTSLHE AALGEIDKAR GANALSTDLQ DVYRAAFQGA GETLYVRRGY IQAAKVDEDA QTL TMADAA ADGVTDDAVG LIIEHVINNG GKAVFMPQEM MSADQPIALV TRY

UniProtKB: Methyl-accepting chemotaxis protein

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Macromolecule #2: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 14.27847 KDa
SequenceString:
MERNYGTGRR KTSTARVFLA KGTGSIVVNG KPLDEYFSRE TSRMVVRQPL ELLDSPTAYD LYITVKGGGI SGQAGAIRHG ITRALIELD DTNKPALKAA GFVTRDSRQV ERKKLGLRKA RKRPQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #3: Elongation factor Tu

MacromoleculeName: Elongation factor Tu / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 43.108789 KDa
SequenceString: MAKAKFERLK PHVNVGTIGH VDHGKTTLTA AIATVAAITS GGEAKDYASI DSAPEEKARG ITINTSHVEY DTPSRHYAHV DCPGHADYV KNMITGAAQM DGAILVVSAT DGPMPQTREH ILLSRQVGVP YIVVFMNKCD MVDDEELLEL VEMEVRELLS D YDFPGDDT ...String:
MAKAKFERLK PHVNVGTIGH VDHGKTTLTA AIATVAAITS GGEAKDYASI DSAPEEKARG ITINTSHVEY DTPSRHYAHV DCPGHADYV KNMITGAAQM DGAILVVSAT DGPMPQTREH ILLSRQVGVP YIVVFMNKCD MVDDEELLEL VEMEVRELLS D YDFPGDDT PIVKGSATEA LKGSQEKYGQ PAVVELLNIL DTYIPEPERD IDKAFLMPIE DVFSISGRGT VVTGRVESGI VK VGDEIEI VGIRDTQKTT CTGVEMFRKL LDEGRAGENC GVLLRGTKRE DVQRGQVLAK PGSITPHTKF DAEVYVLSKE EGG RHTPFL NGYRPQFYFR TTDVTGAIQL QDGTEMVMPG DNVEMGVELI HPIAMDKGLR FAIREGGRTV GAGVVANVNV

UniProtKB: Elongation factor Tu

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Macromolecule #5: Small ribosomal subunit protein bS18

MacromoleculeName: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 8.973312 KDa
SequenceString:
MARFYRRRKF CRFTAEGITH IDYKDVELLK QYISENGKIV PSRITGTSTK YQRQLATAIK QARYLSLLPY TDNHQG

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #6: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 29.465469 KDa
SequenceString: MATKNPTKIE MRDLLQAGAH FGHQTRFWNP KMGPYIFGAR NKIHIINLEH TVKAFNEALN YVNGLASKKN KVLFVGTKRA ASGIIAEQA TRAGMPYVDH RWLGGMLTNW KTLRQSINRL KELEKQAEDG TFAKLTKREA LERTRDMQKL ERSLGGIKDM G GLPDAIFV ...String:
MATKNPTKIE MRDLLQAGAH FGHQTRFWNP KMGPYIFGAR NKIHIINLEH TVKAFNEALN YVNGLASKKN KVLFVGTKRA ASGIIAEQA TRAGMPYVDH RWLGGMLTNW KTLRQSINRL KELEKQAEDG TFAKLTKREA LERTRDMQKL ERSLGGIKDM G GLPDAIFV VDVDHEAIAI KEAKNLGIPV IGIVDTNSNP DNVDYIIPAN DDAIRAVSLY VTAMADAIIA GKEYAQTQAS GN AADAEQE VEQAATAEEA ADVATPVEAK AE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #7: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 18.192918 KDa
SequenceString:
MARNDKNDKN EQTDGLVERL VTVDRVAKVV KGGRIFSFTA LTVVGDGNGR VGFGRGKARE VPAAIQKALE AAKRNMITVE LNDATLHHP IKARHGASKV YMQPASEGTG VIAGGAMRAV LEVAGVKDVL TKCYGSTNTA NVVRATFNGL RDMSTPEKMA A KRGKSVDE ILG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #8: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.753143 KDa
SequenceString:
MATTNQLIRK GRKTIKEKSK VPALQACPQR RGVCTRVYTT TPKKPNSAMR KVCRVRLTSG YEVSSYIGGE GHNLQEHSVV LIRGGRVKD LPGVRYHTVR GALDCAGVKD RKQGRSKYGA KKPKA

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #9: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 19.841166 KDa
SequenceString:
MARLKALYNN ELKQKIKEEL GLDNVMQVPK ITKITLNMGV GGASQDKKLL EGAVADMTAI AGQKPVVTKA RKSIAGFKIR EEWPIGCKV TLRGEQMYEF LDRLIAVAIP RVRDFRGFSP KAFDGRGNYS LGIKEQIVFP EVDFDKIDRI RGMDVTITTS A ATDDEGRA LLKAFGFPFK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #11: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 21.971307 KDa
SequenceString: MDLKTVTGAA VELSDTAFGR EFNEALVHQV VTAYLAGGRQ GTRAQKTRAE VSGGGIKPWR QKGTGRARAG SIRSPIWRGG GRAFAAKPQ DWSQKVNRKM YRGAMQCILA ELIRQERLIL VEELTISAPK TKELIAKLSE LNAPRALIIT KEVDENLYLA A RNIPHVNV ...String:
MDLKTVTGAA VELSDTAFGR EFNEALVHQV VTAYLAGGRQ GTRAQKTRAE VSGGGIKPWR QKGTGRARAG SIRSPIWRGG GRAFAAKPQ DWSQKVNRKM YRGAMQCILA ELIRQERLIL VEELTISAPK TKELIAKLSE LNAPRALIIT KEVDENLYLA A RNIPHVNV LDTSEVDPVS LIAFDKVIMS VEAAKQFEEA LA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #12: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 7.169215 KDa
SequenceString:
MAVQKSRKSR SRRDMRRSHH HMTVAELSVD ATTGEKHRRH HMTKDGFYRG RQLFKVSQEA

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #13: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.635945 KDa
SequenceString:
MRHRKSGVKL GRTSSHRKAM FQNMTNSLFE HELIKTTLPK AKELRRVAEP LITMAKEDSV ANRRLAFSRM RSKTMVGKLF GTLGPRYQN RPGGYLRIVK CGNRDGDNAP MAYVELVDRD

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #14: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 23.887184 KDa
SequenceString: MSINHFALNA VDRSAELQGK GASRRLRKQN LVPAIIYGGN EEPASISIKI NELVKALEFE AFFSHILTIT VDGVEEQAVI KALQRHPAK GFPMHADFQR IVKGQKINMN VPLHFTGAED APGTKKSGIL STVVTDVEIV CLPSQLPEYL AVDVSGMEIG D LFRLSDIK ...String:
MSINHFALNA VDRSAELQGK GASRRLRKQN LVPAIIYGGN EEPASISIKI NELVKALEFE AFFSHILTIT VDGVEEQAVI KALQRHPAK GFPMHADFQR IVKGQKINMN VPLHFTGAED APGTKKSGIL STVVTDVEIV CLPSQLPEYL AVDVSGMEIG D LFRLSDIK LPEGVILFDL DMEDAHDRTI VNMQAPTVEE VDTATVDASE VPATAQTKDA E

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #15: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 9.16879 KDa
SequenceString:
MSRVCQVTGK RPMVGNNVSH ANNKTRRRFL PNLHHHRFWV ESENRFVRLR ISTKGMRIID KLGIDKVLVD LRAQGQKV

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #16: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 7.519604 KDa
SequenceString:
MKISELRDKS LEELTQLLDE KQLDAFRIRM AKATGQLGNT HEVRVNRRAI AQLQTLINEK QRGDS

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #17: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 11.932995 KDa
SequenceString:
MEVTAKLRGA AISAQKVRLV ADEVRGKSIE RALDILTYSN KKGAVFVKKC LNSAIANAEH NHGLDIDGLK VATIYVDEGI TLKRILPRA KGRADRISKR TCHITIKVGE

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #18: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 15.839572 KDa
SequenceString:
MRHYEVVLLV HPDQSDQVVG MVERYIKLVQ DNNGIIHRLE DWGRRQLAYP INKIHKAHYV LFNIETNGET LAELEELFRY NDAIIRSLV MRRDDAITEE SQLAKNADEK RARKATTRRP DSRENDNDDN DHSED

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #19: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 9.119537 KDa
SequenceString:
MAHKKAAGSS RNGRDSNPKM LGVKIFGGQA IVAGNIIVRQ RGTEFHAGAG VGMGRDHTLF ALNDGVVKFA TKGKFNRRYV MVEEA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #20: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 11.465125 KDa
SequenceString:
MSKLRKGDTV IVIAGKDKGK QGTVQAVKND RIKVEGINIV TKHQKPNQAT GVEGGILKQE AFLHISNVAI LNAQTQKADR ITYQFGEDG KKQRVYRSNG EVVATA

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #21: Large ribosomal subunit protein bL31B

MacromoleculeName: Large ribosomal subunit protein bL31B / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 10.708704 KDa
SequenceString:
MRKDIHPDYH DVLFHDTNAD VFFLTRSTVK TKTTREYEGS EYPYYPLDIS SASHPFYTGE QRKTSTEGRV ASFNKRFGAF GGRNKKADT DSAE

UniProtKB: Large ribosomal subunit protein bL31B

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Macromolecule #22: Small ribosomal subunit protein uS14

MacromoleculeName: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 11.777817 KDa
SequenceString:
MAKKSMINRE LKREKEVAKY ADKRTKLKET ISDMNASDEV RMDAMLELQA LPRNSSPVRL RNRCAITGRP HGYFRKFGLS RNMLRERVM QGDVPGVRKA SW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #23: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 10.218672 KDa
SequenceString:
MLTNSDREQI IAQYQRGEND TGSPEVQVAL LSARINDLQN HFKAHKADHH SRRGLIRMVN TRRKLLDYLK GKDLGRYTTL ISQLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #24: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.244396 KDa
SequenceString:
MARIAGVNIP DNKHAVISLT YIFGVGRTTA QKILEAVGIA PTTKVSQLDD IQLDAIRAQV ANYMTEGDLR REVSMNIKRL VDLGCYRGI RHRRNLPVRG QNTKNNARTR KGPTRPLKR

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #25: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.539965 KDa
SequenceString:
MARVKRGVQA NRRHKKILKR AKGYYGARSR VYRVAVQAVT KAGQYAYRDR RNKKRTFRRL WIARINAGAR LNGLSYSRFI NGMKKANIT IDRRVLADIA MHDAATFGAL VEKAKAELA

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #26: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 10.336156 KDa
SequenceString:
MPRSLKKGPF IDAHLFAKVE NALETNSRKP IKTWSRRSMI LPQMVGLTLS VHNGRTHVPV IVSEQMVGHK LGEFAPTRTY RGHGIDRKA KR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #27: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 14.086257 KDa
SequenceString:
MSMQDTVGDM LTRIRNAQMA NKVSVAMPSS KLRKSIADLL VSEGYVASAV VNAEENNKAT LSIELKYFEG KAVIETIQRF SRPGLRQHR GKDAIPTVKQ GMGVAIVSTS QGIMSDRAAR AAGIGGEVVA FVA

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #28: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 19.536309 KDa
SequenceString:
MSRVAKAPVT LPNGVSVTLN DRQVEVKGKN GNLSLRLHDL VELKQEDDVV TLSPTVDSKE AMMHTGTIRA LLNNYVIGVT EGFERRLQL IGVGYRAQAT GNKVTLNVGY SHPVEYTLPE GVSAETPTQT EIVLKSNDKQ QLGQAAAKIR GFRPPEPYKG K GIRYSDEH VIRKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #29: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 17.760559 KDa
SequenceString:
MPRRRVVATR EILPDPKFGS QTIAKFINHV MSHGKKSTAE RIVYGALETV AQKRNIEDPV AFFEDVLENV RPMVEVKARR VGGATYQVP MEVRPSRRTA LAMRWLAEAA AKRSEKSMAL RLAGELSDAS EGKGNAMKKR DEVHRMADAN KAFSHYRF

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #30: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 27.12034 KDa
SequenceString: MGQKVHPIGI RLGVVKKHNA NWYASPKQYS EYLINDIQVR EYLRKKLDSA MISNIMIERP TGAAKITIAT ARPGIVIGKK GEDIERLQK ELTKMMGVPA QVNIEEITSP DLDARLVADG IGSQLERRVM FRRAMKRAVQ NSMRSGAKGI KVELSGRLGG A EIARTEWY ...String:
MGQKVHPIGI RLGVVKKHNA NWYASPKQYS EYLINDIQVR EYLRKKLDSA MISNIMIERP TGAAKITIAT ARPGIVIGKK GEDIERLQK ELTKMMGVPA QVNIEEITSP DLDARLVADG IGSQLERRVM FRRAMKRAVQ NSMRSGAKGI KVELSGRLGG A EIARTEWY REGRVPLHTL RADIDYASVR AETTYGTIGV KVWIFRGEIL DGMNSVYNPV KEEQTRAPKR RGRGGNRRNS DR G

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #31: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.676589 KDa
SequenceString:
MAKDTRSRKK VTRRSVSEGI AHIHASFNNT IVTITDRQGN ALAWATSGGQ GFRGSRKSTP FAAQVAAEVA GKAAQEYGVK NIDVLVKGP GPGRESAVRA LGALGYKVNS ISDVTPIPHN GCRAPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #32: Small ribosomal subunit protein bS21

MacromoleculeName: Small ribosomal subunit protein bS21 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 8.548242 KDa
SequenceString:
MPAVKVKENE PVDIAIRRFK RACEKAGVLS DVRKREFYEK PTQVRKRKKA AAVKRYKKKL QRETIRTTRM Y

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #33: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 6.615821 KDa
SequenceString:
MKTMKVTQLK SGAHRLKSHK ASLKGLGLRR INHSVVVEDT PSTRGMVNRV NYLVKVEEA

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #34: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 15.802331 KDa
SequenceString:
MKTLSAKPAE VTHDWFVVDA DGKTLGRLAT QIASRLRGKH KTNYTPHVDT GDFIVVINAE KIAVTGKKAQ DKKYYRHSGY PGGIKETNF TKLIAHKPED VLHKAVKGML PKGPLGYAMI KKLKLYAGTD HPHEAQQPKE LDI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #35: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 11.635426 KDa
SequenceString:
MANQRIRIRL KSFDHRLIDQ SAQEIVDTAK RTGAQVCGPV PLPTRIERFN VLTSPHVNKD ARDQYEIRTH KRMVDIVQPT DKTVDALMK LDLAAGVDVQ IALG

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #36: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 10.362991 KDa
SequenceString:
MSDNNQATTN ASVLTGRVVS DKMDKSITVL IERLVRHPLY GKQLRRSTKI KAHDENNVCQ QGDLVRIKET RPISKTKAWT LVDVVEKVE KI

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #37: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 5.292455 KDa
SequenceString:
MKRTFQPSVI KRKRTHGFRA RMATKKGRQV LARRRAKGRH RLTV

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #38: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 11.773672 KDa
SequenceString:
MYAVIKTGGK QHRVVVDELL KVELLKAEQG ETITFNDVLM VVDGETVKIG QPVVDGASVE VEVIEHGRGE KIRIVKHNRR KHYHKEQGH RQWYTLLKIK AINA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #39: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 12.550402 KDa
SequenceString:
MFDKKAARLR RAKKTRAHIR FLGVYRLTVN RTPKHIYAQI ISPNGGEVIA QASTLDGSLR SGATGNADAA TSVGQLIAER AKAAGITKV AFDRSGFKYH GRVKALAEAA RENGLEF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #40: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 15.448876 KDa
SequenceString:
MGLRLNELSP GVGAKKTAQR RGRGIGSGLG KTGGRGVKGQ KSRSGSSIRA GFEGGQTPLY RRLPKFGFTS KMAMTTAEVR LSELNKVEG DVISVATLKT ANIIRHDMKR ARIILSGDVT KAYTFQGVKV TKGAKQAIEA AGGSIEE

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #41: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 13.417765 KDa
SequenceString:
MIQVESMLEV ADNSGARRVQ CIKVLGGSHR RYASVGDIIK VTVKEAIPRG RVKKGDVMNA VVVRTKKGVR RPDGSVLRFD DNAAVLLNQ NKAPIATRIF GPVTRELRGD QFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #42: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 22.236305 KDa
SequenceString: MAIGLVGKKC GMTRVFTEAG ASIPVTVVEI SANRITQVKN TDVDGYQAIQ VTTGTRRDSR VTAAQKGHFA KAGVAAGRGV WEFRANDSD LEGREIGGEI LADLFEQGQM VDVTGNSKGK GFQGGVKRHN FSMQDATHGN SVSHRAIGST GQNQSPGKVF K GKKMPGQM ...String:
MAIGLVGKKC GMTRVFTEAG ASIPVTVVEI SANRITQVKN TDVDGYQAIQ VTTGTRRDSR VTAAQKGHFA KAGVAAGRGV WEFRANDSD LEGREIGGEI LADLFEQGQM VDVTGNSKGK GFQGGVKRHN FSMQDATHGN SVSHRAIGST GQNQSPGKVF K GKKMPGQM GNKRVTVQGL EVISVDVEKG LLVIKGAIPG ATGGDVIVRP SVKA

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #43: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 15.626741 KDa
SequenceString:
MLQPKRTKFR KMHKGRNTGL AHRGSTVAFG QFGLKSLTRG RMTARQIEAA RRTITRKIKR GGKIWIRVFP DKPISNKPLE VRMGKGKGP VEYWVCEIKP GKMLYELEGI SEELAREALT LAAAKLPFKT TIVKRTIM

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #44: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 7.594275 KDa
SequenceString:
MKVKMKTKSG AAKRFKRTAN GFKRKQAFKS HILTKKSSKR IRQLRGLKMV DKSDEAAVRR MCPYI

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #45: Large ribosomal subunit protein bL19

MacromoleculeName: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 14.598023 KDa
SequenceString:
MSNKHLLVQA VENAQIIERP SFGPGDTVVV QVKVREGERE RLQAFEGIVI AKRNRGLNSA FTVRKISSGV GVERAFQLHS PIIESIEVK RRGAVRRAKL YYLRERSGKS ARIREKLAPR APKVVKPKAD A

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #46: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 9.955363 KDa
SequenceString:
MVVIRLARGG AKKRPFYQVV VADQRRARDG RYIENIGFFN PLAKESEEAV RLNMEAYHAW IAKGAQPSDR VASLVRAYNK AATTTEATA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #47: Large ribosomal subunit protein bL33

MacromoleculeName: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 6.053298 KDa
SequenceString:
MRDKIKLVST AGTGYYYTTT KNKRTMPGKM EMKKFDPKIR QHVLFKEAKI K

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #48: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 30.263127 KDa
SequenceString: MPIVKAKPTS PGRRFVEKVV HPHLYKGRPF AALLESKSKT GGRNNNGRIT TRHIGGGHKQ HYRIIDFKRT KDNIPATVER IEYDPNRTA HIALLKYADG ERRYIIAAKK QAVGDTVMSG EASPIRPGNC LPLKNIPLGT VIHNIELKIG KGAQMARAAG A SVQLLGRD ...String:
MPIVKAKPTS PGRRFVEKVV HPHLYKGRPF AALLESKSKT GGRNNNGRIT TRHIGGGHKQ HYRIIDFKRT KDNIPATVER IEYDPNRTA HIALLKYADG ERRYIIAAKK QAVGDTVMSG EASPIRPGNC LPLKNIPLGT VIHNIELKIG KGAQMARAAG A SVQLLGRD GIYAILRLRS GETRRVHVNC RAVIGEVSNT ENNLKSLGKA GASRWRGIRP SVRGVAMNPV DHPHGGGEGR NK GRHPTSP WGQKSKGLKT RHNKRTDSMI IRRAKKK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #49: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 24.279043 KDa
SequenceString: MARYIGPKLK LSRREGTDLG LKSGVKPYDV KTKKAARPPG QHGVSRNKTS EYALQLREKQ KVKRIYGVLE RQFANYYKEA ARKRGATGE NLLAMLESRL DNVVYRMGFG STRAEARQLV SHRTVMVKKA GRDEFVRVNI PSIQLQDGDV IAIQEKSREQ L RIKNAIEL ...String:
MARYIGPKLK LSRREGTDLG LKSGVKPYDV KTKKAARPPG QHGVSRNKTS EYALQLREKQ KVKRIYGVLE RQFANYYKEA ARKRGATGE NLLAMLESRL DNVVYRMGFG STRAEARQLV SHRTVMVKKA GRDEFVRVNI PSIQLQDGDV IAIQEKSREQ L RIKNAIEL ATQRGIPEWL DVDHSKLQGT FKKAPDRIDL PAEINESLIV ELYSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #50: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 12.816646 KDa
SequenceString:
MNNARLYQVL RGPVFSEKSQ MLGDSLGVQV FKIDSKATKL EVKKAVELMF EGVEVLKVNT LNVKGKTKRF GKNIGRRNDY KKAYVTLKA GHDVQMADAG EEVANTTAST SETANNE

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #51: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 9.980646 KDa
SequenceString:
MANTAQARKR ARQNTKRRQN SASQRSMVRT YLKRVDAAIA AKDYDAATEA YKKAIPVLDR MADKGIIHKN KAARRKSRLN KTIKGLQA

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #52: Large ribosomal subunit protein bL36

MacromoleculeName: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 4.330293 KDa
SequenceString:
MKVQASVKKI CGSCKVVRRK GRVHIICTAE PRHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #55: Large ribosomal subunit protein bL9

MacromoleculeName: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 18.195211 KDa
SequenceString:
MQIILLQRIV NLGKLGETVD VKPGYGRNFL IPLGKALPAT AANIEKFEAR RAELEAEEKK ELDAAQERAD ALTDVNVIMR AKSGDEGKL FGSIGTRDIA EALTNSGLEV DRAEIKLPEG ALRQVGEYNV DIQLHHDVTA TILVTILSED GDDQVEEDDN E GDYSEE

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #4: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 933.562125 KDa
SequenceString: GUUGUAUGGU CAAGUAAUGA AGCGCACAUG GUGGAUGCCU UGGCAGUCAG AGGCGAUGAA AGACGUGACA GCCUGCGAUA AGCUUCGGG GAGGCGGCAA UAUCCUGUGA UCCGGAGAUU UCUGAAUGGG GAAACCCACU UACCAUAAGG UAGGUAUCUU G UACUUGUA ...String:
GUUGUAUGGU CAAGUAAUGA AGCGCACAUG GUGGAUGCCU UGGCAGUCAG AGGCGAUGAA AGACGUGACA GCCUGCGAUA AGCUUCGGG GAGGCGGCAA UAUCCUGUGA UCCGGAGAUU UCUGAAUGGG GAAACCCACU UACCAUAAGG UAGGUAUCUU G UACUUGUA CAAGAAGCGA ACGAGGGGAA GUGAAACAUC UCAGUACCCU UAGGAAAAGA CAUCAAAUGA GAUUCCCCAA GU AGCGGCG AGCGAACGGG GAGAAGCCGA UUAAUGCUAA UGUAGAAGAA CAGCGUGGGA AAGCUGACCG UAGUAGGUGA UAG UCCUGU AUUCGAAACA UUAGCGUUAA CAUAUUAAGU AGGGCGGGAC ACGAGAAAUC CUGUCUGAAG AUGGGGGGAC CAUC CUCCA AGGCUAAAUA CUCCUGACUG ACCGAUAGUG AACCAGUACC GUGAGGGAAA GGCGAAAAGA ACCCCUGUGA GGGGA GUGA AAUAGAACCU GAAACCGUGU GCGUACAAGC AGUAGGAGCC UCAAUUUAUU GGGGUGACUG CGUACCUUUU GUAUAA UGG GUCAGCGACU UAUAUUCUGU AGCAAGGUUA ACCGUUUAGG GGAGCCGUAG GGAAACCGAG UCUUAAUAGG GCGUCUA GU UGCAGGGUAU AGACCCGAAA CCGAGUGAUC UAUCCAUGAG CAGGUUGAAA GUGCCGUAAC AGGCACCGGA GGACCGAA C CCACUGUCGU UGAAAAGCCA GGGGAUGACU UGUGGAUAGG GGUGAAAGGC UAAUCAAACU CGGUGACAGC UGGUUCUCC CCGAAAGCUA UUUAGGUAGC GCCUCGGACG AAUACCAUUG GGGGUAGAGC ACUGUUUCGG CUAGGGGGUC AUACCGACUU ACCAAACCG AUGCAAACUC CGAAUACCGA UGAGUAAUAU CCGGGAGACA CACGGCGGGU GCUAACGUCC GUCGUGGAGA G GGAAACAA CCCAGACCGC CAGCUAAGGC CCCAAAUUCC UAGUUAAGUG GGAAACGAGG UGGGAAGGCA UAGACAGCUA GG AGGUUGG CUUAGAAGCA GCCAUCCUUU AAAGAAAGCG UAAUAGCUCA CUAGUCGAGU CGGCCUGCGC GGAAGAUGUA ACG GGGCUC AAACUAGGAG CCGAAGCUGC GGAUUUGAAA CUUGUUUUCA AGUGGUAGGG GAGCGUUGUG UAAGCCUGUG AAGG UGUGU CGUAAGGCAU GCUGGAGGUA UCACAAGAGC GAAUGCUGAC GUGAGUAACG AUAAUGCGAG UGAAAAGCUC GCACG CCGG AAGAUCAAGG GUUCCAGUCC AACGUUAAUC GGGGCUGGGU GAGUCGACCC CUAAGGCGAG GCCGAAAGGC GUAGUC GAU GGGAAAUUGG UUAAUAUUCC AAUACUUGUU UAUAGUGCGA UGGAGGGACG GAGAAGGUUA UGUCAGCCUG GCGUUGG UU GUCCAGGUGA AAGGAUGUAG GCUUACAGUU UAGGCAAAUC CGGACUGUAA UAUGGCUGAG AUCUGAUAGC AAGCUGUA C UUGUACAGCG AAGUGGCAAA UACCAUGCUU CCAGGAAAAG CUUCUAAGCA AUAGCUAUAA ACGAAUCGUA CCCUAAACC GACACAGGUG AUCAGGUAGA GAAUACCAAG GCGCUUGAGA GAACUCUGCU GAAGGAACUA GGCAAAAUGG UACCGUAACU UCGGGAGAA GGUACGCUGU UGAUGGUGAU AGGACUUUCU CCUUGAGCUU UCGACAGUCG CAGAUACCAG GCUGCUGCAA C UGUUUAUU AAAAACACAG CACUCUGCAA ACACGAAAGU GGACGUAUAG GGUGUGAUGC CUGCCCGGUG CUGGAAGGUU AA UUGAUGG GGUUAGCGUA AGCGAAGCUC UUGAUCGAAG CCCCAGUAAA CGGCGGCCGU AACUAUAACG GUCCUAAGGU AGC GAAAUU CCUUGUCAGG UAAGUUCUGA CCUGCACGAA UGGCAUAAUG AUGGCAGCGC UGUCUCCAGC AGAGACUCAG UGAA AUCGA AAUCGCAGUG AAGAUGCUGU GUACCCGCGG CUAGACGGAA AGACCCCGUG AACCUUUACU ACAGCUUUAC AUUGA ACUU UGACCUGACU UGUGCAGGAU AGGUGGGAGG CUUUGAAGCC GAGACGCUAG UCUCGGUGGA GCCAAUCUUG AAAUAC CAC CCUGGUCAUG UCGGGGUUCU AACUCAGGUA UAACAAUACC GAGGACAAUG UAUGGUGGGU AGUUUGACUG GGGCGGU CU CCUCCUAAAG AGUAACGGAG GAGUACGAAG GUGCGCUCAG AACGGUCGGA AAUCGUUCAU AGAGUAUAAA GGCAAAAG C GCGCUUAACU GCGAGACCCA CAAGUCGAGC AGGUACGAAA GUAGGUCUUA GUGAUCCGGU GGUUCUGUAU GGAAGGGCC AUCGCUCAAC GGAUAAAAGG UACUCUGGGG AUAACAGGCU GAUACCGCCC AAGAGUUCAU AUCGACGGCG GUGUUUGGCA CCUCGAUGU CGGCUCAUCU CAUCCUAGGG CUGAAGCAGG UCCUAAGGGU AUGGCUGUUC GCCAUUUAAA GAGGUACGCG A GCUGGGUU UAGAACGUCG UGAGACAGUU CGGUCCCUAU CUACCGUGGG CGUUGGAAAU UUGAGAGGAU CUGCUCCUAG UA CGAGAGG ACCAGAGUGG ACGAACCUCU GGUGUUCGGG UUGUCACGCC AGUGGCAUUG CCCGGUAGCU ACGUUCGGAU GGG AUAACC GCUGAAAGCA UCUAAGCGGG AAGCCCACCU CAAGAUUAGA UUUCCCUAAA GAGCCGUUGA AGACUACGAC GUUG AUAGG CAGGGUGUGG AAGUGCAGCG AUGCAUGUAG CUAACCUGUA CUAAUUGCUC GUUUGGCUUG ACCAUACAAC ACCCA AG

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Macromolecule #10: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 36.948945 KDa
SequenceString:
GCUGACGACC AUAGCACGAU GGCACCACCU GAUCCCUUCC CGAACUCAGA AGUGAAACAU CGUCGCGCCA AUGGUAGUGU GGUUUCGAC CAUGUGAGAG UAGGUCAUCG UCAGCU

GENBANK: GENBANK: CP012678.1

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Macromolecule #53: Heterogenous tRNA

MacromoleculeName: Heterogenous tRNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 23.267762 KDa
SequenceString:
UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUACCA

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Macromolecule #54: Heterogenous mRNA

MacromoleculeName: Heterogenous mRNA / type: rna / ID: 54 / Number of copies: 1
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 1.792037 KDa
SequenceString:
UUUUUU

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Macromolecule #56: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 56 / Number of copies: 1
Source (natural)Organism: Psychrobacter urativorans (bacteria)
Molecular weightTheoretical: 514.94975 KDa
SequenceString: GAUGAGCCAA GUUUAGAAGC UUCUUUAAAG AGCUUCAUAG CAAGAUUAAA CUGAAGAGUU UGAUCAUGGC UCAGAUUGAA CGCUGGCGG CAGGCUUAAC ACAUGCAAGU CGAGCGGUAA CAGGAGAAGC UUGCUUCUUG CUGACGAGCG GCGGACGGGU G AGUAAUAC ...String:
GAUGAGCCAA GUUUAGAAGC UUCUUUAAAG AGCUUCAUAG CAAGAUUAAA CUGAAGAGUU UGAUCAUGGC UCAGAUUGAA CGCUGGCGG CAGGCUUAAC ACAUGCAAGU CGAGCGGUAA CAGGAGAAGC UUGCUUCUUG CUGACGAGCG GCGGACGGGU G AGUAAUAC UUAGGAAUCU ACCUAGUAGU GGGGGAUAGC UCGGGGAAAC UCGAAUUAAU ACCGCAUACG ACCUACGGGA GA AAGGGGG CAACUUGUUG CUCUCGCUAU UAGAUGAGCC UAAGUCGGAU UAGCUAGAUG GUGGGGUAAA GGCCUACCAU GGC GACGAU CUGUAGCUGG UCUGAGAGGA UGAUCAGCCA CACCGGGACU GAGACACGGC CCGGACUCCU ACGGGAGGCA GCAG UGGGG AAUAUUGGAC AAUGGGGGCA ACCCUGAUCC AGCCAUGCCG CGUGUGUGAA GAAGGCCUUU UGGUUGUAAA GCACU UUAA GCAGUGAAGA AGACUCCGUG GUUAAUACCC ACGGACGAUG ACAUUAGCUG CAGAAUAAGC ACCGGCUAAC UCUGUG CCA GCAGCCGCGG UAAUACAGAG GGUGCAAGCG UUAAUCGGAA UUACUGGGCG UAAAGGGAGC GUAGGUGGCU CGAUAAG UC AGAUGUGAAA UCCCCGGGCU UAACCUGGGA ACUGGCAUCU GAUACUGUUG AGCUAGAGUA UGUGAGAGGA AGGUAGAA U UCCAGGUGUA GCGGUGAAAU GCGUAGAGAU CUGGAGGAAU ACCGAUGGCG AAGGCAGCCU UCUGGCAUAA UACUGACAC UGAGGCUCGA AAGCGUGGGU AGCAAACAGG AUUAGAUACC CUGGUAGUCC ACGCCGUAAA CGAUGUCUAC UAGUCGUUGG GUCCCCUUG AGGACUUAGU GACGCAGCUA ACGCAAUAAG UAGACCGCCU GGGGAGUACG GCCGCAAGGU UAAAACUCAA A UGAAUUGA CGGGGGCCCG CACAAGCGGU GGAGCAUGUU GUUUAAUUCG AUGCAACGCG AAGAACCUUA CCUGGUCUUG AC AUAUCUA GAAUCCUGCA GAGAUGCGGG AGUGCCUUCG GGAAUUAGAA UACAGGUGCU GCAUGGCUGU CGUCAGCUCG UGU CGUGAG AUGUUGGGUU AAGUCCCGCA ACGAGCGCAA CCCUUGUCCU UAGUUACCAG CGGGUUAAGC CGGGAACUCU AAGG AUACU GCCAGUGACA AACUGGAGGA AGGCGGGGAC GACGUCAAGU CAUCAUGGCC CUUACGACCA GGGCUACACA CGUGC UACA AUGGUAGGUA CAGAGGGCAG CUACACAGCG AUGUGAUGCG AAUCUCAAAA AGCCUAUCGU AGUCCCGGAU UGGAGU CUG CAACUCGACU CCAUGAAGUA GGAAUCGCUA GUAAUCGCGG AUCAGAAUGC CGCGGUGAAU ACGUUCCCGG GCCUUGU AC ACACCGCCCG UCACACCAUG GGAGUUGAUU GCACCAGAAG UGGAUAGCUU AACCUUCGGG AAGGCGUUCA CCACGGUG U GGUUGAUGAC UGGGGUGAAG UCGUAACAAG GUAGCCGUAG GGGAACCUGC GGCUGGAUCA CCUCCUUAU

GENBANK: GENBANK: CP012678.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.75 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.9 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 30469
Initial angle assignmentType: OTHER
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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