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- EMDB-18162: N5-methyl-H4MPT:CoM methyltransferase -coenzyme M complex + CoM -
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Open data
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Basic information
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Title | N5-methyl-H4MPT:CoM methyltransferase -coenzyme M complex + CoM | |||||||||
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![]() | methanogenesis / tetrahydromethanopterin / coenzyme M / vitamin B12 / Na+ transport / TRANSFERASE | |||||||||
Function / homology | ![]() tetrahydromethanopterin S-methyltransferase / tetrahydromethanopterin S-methyltransferase activity / methyltransferase complex / methanogenesis, from carbon dioxide / vesicle membrane / cobalt ion binding / sodium ion transport / one-carbon metabolic process / methylation / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.39 Å | |||||||||
![]() | Aziz I / Vonck J / Ermler U | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the methanogenic Na+ translocating N5-methyl-H4MPT:CoM methyltransferase complex Authors: Aziz I / Kayastha K / Kaltwasser S / Vonck J / Welsch S / Murohy BJ / Kahnt J / Wu D / Wagner T / Shima S / Ermler U | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 97.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 21.7 KB 21.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.3 KB | Display | ![]() |
Images | ![]() | 45.4 KB | ||
Masks | ![]() | 125 MB | ![]() | |
Filedesc metadata | ![]() | 6.6 KB | ||
Others | ![]() ![]() | 98.2 MB 98.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8q54 M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.837 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_18162_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_18162_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Methyl-H4MPT:CoM methyltransferase
+Supramolecule #1: Methyl-H4MPT:CoM methyltransferase
+Macromolecule #1: Tetrahydromethanopterin S-methyltransferase subunit A 1
+Macromolecule #2: Tetrahydromethanopterin S-methyltransferase subunit B
+Macromolecule #3: Tetrahydromethanopterin S-methyltransferase subunit C
+Macromolecule #4: Tetrahydromethanopterin S-methyltransferase subunit D
+Macromolecule #5: Tetrahydromethanopterin S-methyltransferase subunit E
+Macromolecule #6: Tetrahydromethanopterin S-methyltransferase subunit F
+Macromolecule #7: Tetrahydromethanopterin S-methyltransferase subunit G
+Macromolecule #8: MAGNESIUM ION
+Macromolecule #9: [(2~{S},7~{R},11~{R},15~{S},19~{S},22~{S},26~{S},30~{R},34~{R},39...
+Macromolecule #10: SODIUM ION
+Macromolecule #11: 1-THIOETHANESULFONIC ACID
+Macromolecule #12: water
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 73.9 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.1 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |