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Yorodumi- EMDB-15311: Dedicated chaperone at the ribosome safeguards the proteostasis n... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15311 | |||||||||||||||
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Title | Dedicated chaperone at the ribosome safeguards the proteostasis network during eEF1A biogenesis | |||||||||||||||
Map data | overall map, unsharpened | |||||||||||||||
Sample |
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Keywords | CHP1 / Ribosome / Complex | |||||||||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||||||||
Authors | Minoia M / Quintana-Cordero J / Katharina J / Kotan I / Turnbull KJ / Masser AE / Merker D / Gouarin E / Ehrenbolger K / Barandun J ...Minoia M / Quintana-Cordero J / Katharina J / Kotan I / Turnbull KJ / Masser AE / Merker D / Gouarin E / Ehrenbolger K / Barandun J / Hauryliuk V / Bukau B / Kramer G / Andreasson C | |||||||||||||||
Funding support | Sweden, European Union, Estonia, 4 items
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Citation | Journal: To Be Published Title: Dedicated chaperone at the ribosome safeguards the proteostasis network during eEF1A biogenesis Authors: Minoia M / Quintana-Cordero J / Katharina J / Kotan I / Turnbull KJ / Masser AE / Merker D / Gouarin E / Ehrenbolger K / Barandun J / Hauryliuk V / Bukau B / Kramer G / Andreasson C | |||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15311.map.gz | 65.4 MB | EMDB map data format | |
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Header (meta data) | emd-15311-v30.xml emd-15311.xml | 20.5 KB 20.5 KB | Display Display | EMDB header |
Images | emd_15311.png | 79.3 KB | ||
Others | emd_15311_additional_1.map.gz emd_15311_additional_2.map.gz emd_15311_additional_3.map.gz emd_15311_half_map_1.map.gz emd_15311_half_map_2.map.gz | 65.5 MB 65.5 MB 65.5 MB 65.4 MB 65.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15311 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15311 | HTTPS FTP |
-Validation report
Summary document | emd_15311_validation.pdf.gz | 958.6 KB | Display | EMDB validaton report |
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Full document | emd_15311_full_validation.pdf.gz | 958.2 KB | Display | |
Data in XML | emd_15311_validation.xml.gz | 12.8 KB | Display | |
Data in CIF | emd_15311_validation.cif.gz | 15.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15311 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15311 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15311.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | overall map, unsharpened | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.64 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: focused map
File | emd_15311_additional_1.map | ||||||||||||
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Annotation | focused map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: focused half map 1
File | emd_15311_additional_2.map | ||||||||||||
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Annotation | focused half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: focused half map 2
File | emd_15311_additional_3.map | ||||||||||||
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Annotation | focused half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: overall half map 1
File | emd_15311_half_map_1.map | ||||||||||||
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Annotation | overall half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: overall half map 2
File | emd_15311_half_map_2.map | ||||||||||||
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Annotation | overall half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Chp1-80S ribosomal complexes
Entire | Name: Chp1-80S ribosomal complexes |
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Components |
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-Supramolecule #1: Chp1-80S ribosomal complexes
Supramolecule | Name: Chp1-80S ribosomal complexes / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: BY4741 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Details | Data was collected at a pixel size of 0.82 |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average exposure time: 3.0 sec. / Average electron dose: 33.7 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.7 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |