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- EMDB-14867: Dimeric PSI of Chlamydomonas reinhardtii at 2.74 A resolution (sy... -

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Basic information

Entry
Database: EMDB / ID: EMD-14867
TitleDimeric PSI of Chlamydomonas reinhardtii at 2.74 A resolution (symmetry expanded)
Map data
Sample
  • Organelle or cellular component: Photosystem I dimer of Chlamydomonas reinhardtii
    • Protein or peptide: x 20 types
  • Ligand: x 14 types
Function / homology
Function and homology information


chloroplast thylakoid lumen / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chloroplast thylakoid membrane / chlorophyll binding ...chloroplast thylakoid lumen / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chloroplast thylakoid membrane / chlorophyll binding / response to light stimulus / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily ...Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Chlorophyll A-B binding protein, plant and chromista / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Photosystem I PsaD / Photosystem I, reaction centre subunit PsaD superfamily / PsaD / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I reaction centre subunit IX / PsaJ / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / : / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I reaction center subunit VIII / PSI subunit V / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic ...Photosystem I reaction center subunit VIII / PSI subunit V / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center / Chlorophyll a-b binding protein, chloroplastic / Photosystem I reaction center subunit II, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.74 Å
AuthorsNaschberger A / Amunts A
Funding support Sweden, Germany, 4 items
OrganizationGrant numberCountry
The Swedish Foundation for Strategic ResearchARC19-0051 Sweden
Knut and Alice Wallenberg Foundation2018.0080 Sweden
German Federal Ministry for Education and ResearchNRW 28 313-WO44A Germany
German-Israeli Foundation for Research and DevelopmentG-1483- 207/2018 Germany
CitationJournal: Nat Plants / Year: 2022
Title: Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex.
Authors: Andreas Naschberger / Laura Mosebach / Victor Tobiasson / Sebastian Kuhlgert / Martin Scholz / Annemarie Perez-Boerema / Thi Thu Hoai Ho / André Vidal-Meireles / Yuichiro Takahashi / ...Authors: Andreas Naschberger / Laura Mosebach / Victor Tobiasson / Sebastian Kuhlgert / Martin Scholz / Annemarie Perez-Boerema / Thi Thu Hoai Ho / André Vidal-Meireles / Yuichiro Takahashi / Michael Hippler / Alexey Amunts /
Abstract: Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular ...Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microscopy identified that the absence of PsaH and Lhca2 gives rise to a head-to-head relative orientation of the PSI-light-harvesting complex I monomers in a way that is essentially different from the oligomer formation in cyanobacteria. The light-harvesting protein Lhca9 is the key element for mediating this dimerization. The interface between the monomers is lacking PsaH and thus partially overlaps with the surface area that would bind one of the light-harvesting complex II complexes in state transitions. We also define the most accurate available PSI-light-harvesting complex I model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations to all the pigments, as well as 621 water molecules that affect energy transfer pathways.
History
DepositionApr 29, 2022-
Header (metadata) releaseNov 23, 2022-
Map releaseNov 23, 2022-
UpdateNov 23, 2022-
Current statusNov 23, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_14867.png

Downloads & links

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Map

FileDownload / File: emd_14867.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.84 Å/pix.
x 700 pix.
= 588. Å		0.84 Å/pix.
x 700 pix.
= 588. Å		0.84 Å/pix.
x 700 pix.
= 588. Å

Surface

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.84 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.031
Minimum - Maximum-0.07526777 - 0.17508438
Average (Standard dev.)-0.00016791113 (±0.0023204703)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions700700700
Spacing700700700
CellA=B=C: 588.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_14867_msk_1.map
Projections & Slices
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Half map: #2

Fileemd_14867_half_map_1.map
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Half map: #1

Fileemd_14867_half_map_2.map
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Sample components

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Entire : Photosystem I dimer of Chlamydomonas reinhardtii

EntireName: Photosystem I dimer of Chlamydomonas reinhardtii
Components
  • Organelle or cellular component: Photosystem I dimer of Chlamydomonas reinhardtii
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit IV, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit III, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit V, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: PSI subunit V
    • Protein or peptide: Photosystem I reaction center subunit psaK, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic (Lhca4)
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
  • Ligand: CHLOROPHYLL A ISOMER
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONE
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: BETA-CAROTENE
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: DODECYL-ALPHA-D-MALTOSIDE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: CHLOROPHYLL B
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
  • Ligand: water

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Supramolecule #1: Photosystem I dimer of Chlamydomonas reinhardtii

SupramoleculeName: Photosystem I dimer of Chlamydomonas reinhardtii / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#20
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightExperimental: 700 KDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 83.239203 KDa
SequenceString: MTISTPEREA KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLS GMYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL Y TTAIGGLV ...String:
MTISTPEREA KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLS GMYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL Y TTAIGGLV MAAAMFFAGW FHYHKAAPKL EWFQNVESML NHHLGGLLGL GSLAWAGHQI HVSLPVNKLL DAGVDPKEIP LP HDLLLNR AIMADLYPSF AKGIAPFFTL NWSEYSDFLT FKGGLNPVTG GLWLSDTAHH HVAIAVLFLV AGHMYRTNWG IGH SMKEIL EAHRGPFTGE GHVGLYEILT TSWHAQLAIN LALFGSLSII VAHHMYAMPP YPYLATDYGT QLSLFTHHTW IGGF CIVGA GAHAAIFMVR DYDPTNNYNN LLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMSAL GRPQDMFSDT AIQLQ PVFA QWIQNTHFLA PQLTAPNALA ATSLTWGGDL VAVGGKVAMM PISLGTSDFM VHHIHAFTIH VTVLILLKGV LFARSS RLI PDKANLGFRF PCDGPGRGGT CQVSAWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVT ASGVSHITGG NFAQSAN TI NGWLRDFLWA QSSQVIQSYG SALSAYGLIF LGAHFVWAFS LMFLFSGRGY WQELIESIVW AHNKLKVAPA IQPRALSI T QGRAVGVAHY LLGGIATTWS FFLARIISVG

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 82.184266 KDa
SequenceString: MATKLFPKFS QGLAQDPTTR RIWYGLAMAH DFESHDGMTE ENLYQKIFAS HFGQLSIIFL WTSGNLFHVA WQGNFEQWVT DPVHIRPIA HAIWDPHFGQ PAVEAFTRGG ASGPVNISTS GVYQWWYTIG MRTNQDLYVG SVFLALVSAI FLFAGWLHLQ P NFQPSLSW ...String:
MATKLFPKFS QGLAQDPTTR RIWYGLAMAH DFESHDGMTE ENLYQKIFAS HFGQLSIIFL WTSGNLFHVA WQGNFEQWVT DPVHIRPIA HAIWDPHFGQ PAVEAFTRGG ASGPVNISTS GVYQWWYTIG MRTNQDLYVG SVFLALVSAI FLFAGWLHLQ P NFQPSLSW FKDAESRLNH HLSGLFGVSS LAWTGHLVHV AIPESRGQHV GWDNFLSVLP HPQGLTPFFT GNWAAYAQSP DT ASHVFGT AQGSGQAILT FLGGFHPQTQ SLWLTDMAHH HLAIAVIFIV AGHMYRTNFG IGHRMQAILE AHTPPSGSLG AGH KGLFDT VNNSLHFQLG LALASVGTIT SLVAQHMYSL PPYAFQAIDF TTQAALYTHH QYIAGFIMCG AFAHGAIFFI RDYD PEQNK GNVLARMLDH KEALISHLSW VSLFLGFHTL GLYVHNDVMQ AFGTPEKQIL IEPVFAQWIQ AAHGKALYGF DFLLS SKTS AAFANGQSLW LPGWLDAINN NQNSLFLTIG PGDFLVHHAI ALGLHTTTLI LVKGALDARG SKLMPDKKDF GYSFPC DGP GRGGTCDISA YDAFYLAVFW MLNTIGWVTF YWHWKHLTLW QGNVAQFDES STYLMGWLRD YLWLNSSQLI NGYNPFG MN SLSVWAWTFL FGHLIYATGF MFLISWRGYW QELIETLVWA HEKTPLANLV YWKDKPVALS IVQARLVGLA HFSVGYIF T YAAFLIASTS GRFG

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 8.869325 KDa
SequenceString:
MAHIVKIYDT CIGCTQCVRA CPLDVLEMVP WDGCKASQMA SAPRTEDCVG CKRCETACPT DFLSVRVYLG SESTRSMGLS Y

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Macromolecule #4: Photosystem I reaction center subunit II, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit II, chloroplastic
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.372887 KDa
SequenceString: MAVMMRTQAP AATRASSRVA VAARPAARRA VVVRAEAEAA PAAAKKAAEK PAWTVPTLNP DTPSPIFGGS TGGLLRKAQT EEFYVITWE AKKEQIFEMP TGGAAIMRQG PNLLKFGKKE QCLALTTQLR NKFKLTPCFY RVFPDGKVQY LHPADGVYPE K VNAGRVGA ...String:
MAVMMRTQAP AATRASSRVA VAARPAARRA VVVRAEAEAA PAAAKKAAEK PAWTVPTLNP DTPSPIFGGS TGGLLRKAQT EEFYVITWE AKKEQIFEMP TGGAAIMRQG PNLLKFGKKE QCLALTTQLR NKFKLTPCFY RVFPDGKVQY LHPADGVYPE K VNAGRVGA NQNMRRIGQN VNPIKVKFSG RMMSPAEI

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Macromolecule #5: Photosystem I reaction center subunit IV, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit IV, chloroplastic
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.786395 KDa
SequenceString:
MQALSSRVNI AAKPQRAQRL VVRAEEVKAA PKKEVGPKRG SLVKILRPES YWFNQVGKVV SVDQSGVRYP VVVRFENQNY AGVTTNNYA LDEVVAAK

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Macromolecule #6: Photosystem I reaction center subunit III, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit III, chloroplastic
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 24.088936 KDa
SequenceString: MALTMRNPAV KASSRVAPSS RRALRVACQA QKNETASKVG TALAASALAA AVSLSAPSAA MADIAGLTPC SESKAYAKLE KKELKTLEK RLKQYEADSA PAVALKATME RTKARFANYA KAGLLCGNDG LPHLIADPGL ALKYGHAGEV FIPTFGFLYV A GYIGYVGR ...String:
MALTMRNPAV KASSRVAPSS RRALRVACQA QKNETASKVG TALAASALAA AVSLSAPSAA MADIAGLTPC SESKAYAKLE KKELKTLEK RLKQYEADSA PAVALKATME RTKARFANYA KAGLLCGNDG LPHLIADPGL ALKYGHAGEV FIPTFGFLYV A GYIGYVGR QYLIAVKGEA KPTDKEIIID VPLATKLAWQ GAGWPLAAVQ ELQRGTLLEK EENITVSPR

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Macromolecule #7: Photosystem I reaction center subunit V, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit V, chloroplastic
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.236007 KDa
SequenceString:
MQTLASRPSL RASARVAPRR APRVAVVTKA ALDPQIVISG STAAFLAIGR FVFLGYQRRE ANFDSTVGPK TTGATYFDDL QKNSTIFAT NDPAGFNIID VAGWGALGHA VGFAVLAINS LQGANLS

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Macromolecule #8: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.586388 KDa
SequenceString:
MALRAVSAKS AVRPTVARAS VKPVAALKPA QKMALAGAAS VALLAASSSS AEASQVIATV ASAAQGYPFV PPSWAPSVFV PLTGLVLPA IAMATLFVYI EKEAPSS

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Macromolecule #9: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 4.645372 KDa
SequenceString:
(AME)KDFTTYLST APVIATIWFT FTAGLLIEIN RYFPDPLVFS

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Macromolecule #10: PSI subunit V

MacromoleculeName: PSI subunit V / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 20.300539 KDa
SequenceString: MAVAMRSSTG LRATAARRQM PLGLGRVSTV RVCAADTKKA QVISPVNGDP FVGMLETPVT SAPIVATYLS NLPAYRTGVA PVLRGVEIG LAHGFLLAGP FIKLGPLRNV PETAEIAGSL SAAGLVLILA LCLSIYGSAQ FQSTPSIGVK TLSGRSVARD P LFSADGWS ...String:
MAVAMRSSTG LRATAARRQM PLGLGRVSTV RVCAADTKKA QVISPVNGDP FVGMLETPVT SAPIVATYLS NLPAYRTGVA PVLRGVEIG LAHGFLLAGP FIKLGPLRNV PETAEIAGSL SAAGLVLILA LCLSIYGSAQ FQSTPSIGVK TLSGRSVARD P LFSADGWS EFAAGFLVGG EAGVAWAYVC TQILPYYS

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Macromolecule #11: Photosystem I reaction center subunit psaK, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit psaK, chloroplastic
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 11.214084 KDa
SequenceString:
MQALATRPSA IRPTKAARRS SVVVRADGFI GSSTNLIMVA STTATLAAAR FGLAPTVKKN TTAGLKLVDS KNSAGVISND PAGFTIVDV LAMGAAGHGL GVGIVLGLKG IGAL

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Macromolecule #12: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 23.923205 KDa
SequenceString: MALSMRTLSA RTAAPRGFSG RRVAAVSNGS RVTMKAGNWL PGSDAPAWLP DDLPGNYGFD PLSLGKEPAS LKRFTESEVI HGRWAMLGV AGSLAVELLG YGNWYDAPLW AVNGGKATWF GIEVPFDLNA LLAFEFVAMA AAEGQRGDAG GVVYPGGAFD P LGFAKDSS ...String:
MALSMRTLSA RTAAPRGFSG RRVAAVSNGS RVTMKAGNWL PGSDAPAWLP DDLPGNYGFD PLSLGKEPAS LKRFTESEVI HGRWAMLGV AGSLAVELLG YGNWYDAPLW AVNGGKATWF GIEVPFDLNA LLAFEFVAMA AAEGQRGDAG GVVYPGGAFD P LGFAKDSS KSGELKLKEI KNGRLAMVAF LGFVAQHAAT GKGPIAALGE HLANPWGANF ATNGISVPFF

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Macromolecule #13: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 32.629486 KDa
SequenceString: MMLTKSAQAA FSGKVARPAK ANRARLVCRA EEKSIAKVDR SKDQLYVGAS QSSLAYLDGS LPGDFGFDPL GLLDPVNSGG FIEPKWLQY SEVIHARWAM LGAAGCIAPE VLGAAGLIPD ATNIKWFESG VIPPAGSYNG YWADPYTIFF VEIVAMQFAE L RRLQDFRY ...String:
MMLTKSAQAA FSGKVARPAK ANRARLVCRA EEKSIAKVDR SKDQLYVGAS QSSLAYLDGS LPGDFGFDPL GLLDPVNSGG FIEPKWLQY SEVIHARWAM LGAAGCIAPE VLGAAGLIPD ATNIKWFESG VIPPAGSYNG YWADPYTIFF VEIVAMQFAE L RRLQDFRY PGSMGQQYFL GLEAIFKGSG DAAYPGGPFF NLFNLGKTEA AMKELKLKEI KNGRLAMLAM LGYGAQAVMT GK GPFQNLV EHLADPVNNN ILTNFAGRVS GSSQPWRPHG WWRRRYRSVA ALALIRNRSV C

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Macromolecule #14: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.248869 KDa
SequenceString: MALSMIAQRR AGAFSARQAP RAVRAQAAVR PVWFPGNPPP AHLDGSLAGD YGFDPLFLGQ EPQTLKWYVQ AELVHGRFAM LGAAGIILT SIGAKVGLGF PEWYDAGKVV VEKNNIDFPT LMVIQFYLMG WAETKRWYDF KNPGSQADGS FLGFTEEFKG L ENGYPGGR ...String:
MALSMIAQRR AGAFSARQAP RAVRAQAAVR PVWFPGNPPP AHLDGSLAGD YGFDPLFLGQ EPQTLKWYVQ AELVHGRFAM LGAAGIILT SIGAKVGLGF PEWYDAGKVV VEKNNIDFPT LMVIQFYLMG WAETKRWYDF KNPGSQADGS FLGFTEEFKG L ENGYPGGR FFDPMGLSRG DAAKYQEYKQ KEVKNGRLAM IACLGFAAQY AATGKGPLDN LADHLADPNH VNFATNGVSI PI A

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Macromolecule #15: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 25.948812 KDa
SequenceString: MALTMKRSGV AARSASSRKS VVTCVARQSW LPGSQIPAHL DTPAAQALAG NFGFDPLGLG KDPVALRWYQ QAELIHCRTA MAGVAGILI PGLLTKAGAL NVPEWYDAGK VAIENSFAPW GSLLAVQLFL CGFVEAKRWQ DIRKPGSQGE PGSFLGFEAS L KGTSELGY ...String:
MALTMKRSGV AARSASSRKS VVTCVARQSW LPGSQIPAHL DTPAAQALAG NFGFDPLGLG KDPVALRWYQ QAELIHCRTA MAGVAGILI PGLLTKAGAL NVPEWYDAGK VAIENSFAPW GSLLAVQLFL CGFVEAKRWQ DIRKPGSQGE PGSFLGFEAS L KGTSELGY PGGPFDPLGL SKEADKWADW KLKEVKNGRL AMLAFLGFVA QKYATGAGPV DNLAAHLKDP WHVNYATNGV SL PFL

+
Macromolecule #16: Chlorophyll a-b binding protein, chloroplastic (Lhca4)

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic (Lhca4)
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 28.729822 KDa
SequenceString: MAFVLAKSSA FGVAAKPVSR RSSVAVKASA VPENVKEARE WIDAWKSKSG GAKRDAALPS WMPGADLPGY LNGTLPGDFG FDPLYLGQD PVKLKWYAQA ELMNARFAML AVAGILVPEL LSNIGFSWPG AGVAWYDAGK FEYFAPASSL FGVQMLLFAW V EIRRYQDF ...String:
MAFVLAKSSA FGVAAKPVSR RSSVAVKASA VPENVKEARE WIDAWKSKSG GAKRDAALPS WMPGADLPGY LNGTLPGDFG FDPLYLGQD PVKLKWYAQA ELMNARFAML AVAGILVPEL LSNIGFSWPG AGVAWYDAGK FEYFAPASSL FGVQMLLFAW V EIRRYQDF VKPGSANQDP IFTNNKLPDG NEPGYPGGIF DPFGWSKGDI KSLKLKEIKN GRLAMLAFAG FIGQAYTTGT TP LKNLSTH LADPWSTTVW QNDLARL

+
Macromolecule #17: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 28.257555 KDa
SequenceString: MAALMQKSAL SRPACSTRSS RRAVVVRAAA DRKLWAPGVV APEYLKGDLA GDYGWDPLGL GADPTALKWY RQSELQHARW AMLGVAGVL VQEIVKPDVY FYEAGLPQNL PEPFTNINMG GLLAWEFILM HWVEVRRWQD YKNFGSVNED PIFKGNKVPN P EMGYPGGI ...String:
MAALMQKSAL SRPACSTRSS RRAVVVRAAA DRKLWAPGVV APEYLKGDLA GDYGWDPLGL GADPTALKWY RQSELQHARW AMLGVAGVL VQEIVKPDVY FYEAGLPQNL PEPFTNINMG GLLAWEFILM HWVEVRRWQD YKNFGSVNED PIFKGNKVPN P EMGYPGGI FDPFGFSKGN LKELQTKEIK NGRLAMIAYM AFILQAQATG KGPLAALSAH LSNPFGNNIL KNIGTCTVPH SV DVQGLTI PLTCLWPGSQ

+
Macromolecule #18: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.812373 KDa
SequenceString: MMLVAKNAVA ARPSARSARR SVVAKASSRP LWLPGSTPPA HLKGDLPGDF GFDPLGLGAN AESLKWFKES ELVHSRWAMA AVAGILVQE IVRPDVFWYN AGKEVESPLG PLGLLAVEFF LMHWVEVRRW QDLRKPGSVD QDPIFSQYKL PPHEVGYPGG V FAPFIPGD ...String:
MMLVAKNAVA ARPSARSARR SVVAKASSRP LWLPGSTPPA HLKGDLPGDF GFDPLGLGAN AESLKWFKES ELVHSRWAMA AVAGILVQE IVRPDVFWYN AGKEVESPLG PLGLLAVEFF LMHWVEVRRW QDLRKPGSVD QDPIFSQYKL PPHEVGYPGG V FAPFIPGD LAELKVKEIK NGRLAMLAFV GFVMAAQVTG KGPIAALQEH LADPWGTTIF SKAAVVPGQA VAPPCKIPAS VS YKGIEIP TPCFLQGLWP

+
Macromolecule #19: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.867277 KDa
SequenceString: MIAAKSQVAL GRRAPVRGQR VVAAASARPT WLPGLNPPAH LKGALAGDNG FDPLGLGQDE GRLKWYAEAE KTNGRWAMMA VAGILGQEL LGVTPAWWEA GAKEYDIPAQ ALTPIEFIVM GFLEIKRYQG FKQTGTSGFI NSFPFDPAGM NSPSMATKEV K NGRLAMVA ...String:
MIAAKSQVAL GRRAPVRGQR VVAAASARPT WLPGLNPPAH LKGALAGDNG FDPLGLGQDE GRLKWYAEAE KTNGRWAMMA VAGILGQEL LGVTPAWWEA GAKEYDIPAQ ALTPIEFIVM GFLEIKRYQG FKQTGTSGFI NSFPFDPAGM NSPSMATKEV K NGRLAMVA FIGFCVQALA TRTQPIEGLT AHLADPFGKN ITYYLTHLPE TLGSA

+
Macromolecule #20: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 20.637457 KDa
SequenceString:
TKLFPKFSQG LAQDPTTRRI WYGLAMAHDF ESHDGMTEEN LYQKIFASHF GQLSIIFLWT SGNLFHVAWQ GNFEQIAHAI WDPHFGQPA VEAFTRGPVN IGMRTNQDLY VGSVFLALVS AIFLFAGWLH LQPNFQPSLS WFWTGHLVHV AIPHVGWDNF L SVLPHEKT PLANLVYWKD KP

+
Macromolecule #21: CHLOROPHYLL A ISOMER

MacromoleculeName: CHLOROPHYLL A ISOMER / type: ligand / ID: 21 / Number of copies: 1 / Formula: CL0
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CL0:
CHLOROPHYLL A ISOMER

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Macromolecule #22: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 22 / Number of copies: 226 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #23: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 23 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE

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Macromolecule #24: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 24 / Number of copies: 16 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #25: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 25 / Number of copies: 36 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE

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Macromolecule #26: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 26 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #27: DODECYL-ALPHA-D-MALTOSIDE

MacromoleculeName: DODECYL-ALPHA-D-MALTOSIDE / type: ligand / ID: 27 / Number of copies: 34 / Formula: LMU
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

+
Macromolecule #28: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 28 / Number of copies: 17 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #29: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 29 / Number of copies: 20 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

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Macromolecule #30: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 30 / Number of copies: 1 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

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Macromolecule #31: CHLOROPHYLL B

MacromoleculeName: CHLOROPHYLL B / type: ligand / ID: 31 / Number of copies: 32 / Formula: CHL
Molecular weightTheoretical: 907.472 Da
Chemical component information

ChemComp-CHL:
CHLOROPHYLL B

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Macromolecule #32: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 32 / Number of copies: 7 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

+
Macromolecule #33: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...

MacromoleculeName: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
type: ligand / ID: 33 / Number of copies: 2 / Formula: NEX
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

+
Macromolecule #34: water

MacromoleculeName: water / type: ligand / ID: 34 / Number of copies: 88 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 45.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.74 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 28346
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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