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- EMDB-13642: Alpha-latrocrustotoxin monomer -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-13642
TitleAlpha-latrocrustotoxin monomer
Map data
Sample
  • Complex: Alpha-latrocrustotoxin-Lt1a in soluble monomeric state
    • Protein or peptide: Alpha-latrocrustotoxin-Lt1a
KeywordsPore-forming toxin / TOXIN
Function / homology
Function and homology information


other organism cell membrane / host cell presynaptic membrane / exocytosis / toxin activity / extracellular region / membrane
Similarity search - Function
Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily
Similarity search - Domain/homology
Alpha-latrocrustotoxin-Lt1a
Similarity search - Component
Biological speciesLatrodectus tredecimguttatus (black widow)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.03 Å
AuthorsChen M / Gatsogiannis C
Funding support Japan, 3 items
OrganizationGrant numberCountry
Alexander von Humboldt Foundation Japan
Max Planck Society Japan
Japan Society for the Promotion of Science (JSPS) Japan
CitationJournal: Nat Commun / Year: 2021
Title: Molecular architecture of black widow spider neurotoxins.
Authors: Minghao Chen / Daniel Blum / Lena Engelhard / Stefan Raunser / Richard Wagner / Christos Gatsogiannis /
Abstract: Latrotoxins (LaTXs) are presynaptic pore-forming neurotoxins found in the venom of Latrodectus spiders. The venom contains a toxic cocktail of seven LaTXs, with one of them targeting vertebrates (α- ...Latrotoxins (LaTXs) are presynaptic pore-forming neurotoxins found in the venom of Latrodectus spiders. The venom contains a toxic cocktail of seven LaTXs, with one of them targeting vertebrates (α-latrotoxin (α-LTX)), five specialized on insects (α, β, γ, δ, ε- latroinsectotoxins (LITs), and one on crustaceans (α-latrocrustatoxin (α-LCT)). LaTXs bind to specific receptors on the surface of neuronal cells, inducing the release of neurotransmitters either by directly stimulating exocytosis or by forming Ca-conductive tetrameric pores in the membrane. Despite extensive studies in the past decades, a high-resolution structure of a LaTX is not yet available and the precise mechanism of LaTX action remains unclear. Here, we report cryoEM structures of the α-LCT monomer and the δ-LIT dimer. The structures reveal that LaTXs are organized in four domains. A C-terminal domain of ankyrin-like repeats shields a central membrane insertion domain of six parallel α-helices. Both domains are flexibly linked via an N-terminal α-helical domain and a small β-sheet domain. A comparison between the structures suggests that oligomerization involves major conformational changes in LaTXs with longer C-terminal domains. Based on our data we propose a cyclic mechanism of oligomerization, taking place prior membrane insertion. Both recombinant α-LCT and δ-LIT form channels in artificial membrane bilayers, that are stabilized by Ca ions and allow calcium flux at negative membrane potentials. Our comparative analysis between α-LCT and δ-LIT provides first crucial insights towards understanding the molecular mechanism of the LaTX family.
History
DepositionSep 27, 2021-
Header (metadata) releaseDec 8, 2021-
Map releaseDec 8, 2021-
UpdateJul 17, 2024-
Current statusJul 17, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7ptx
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13642.png

Downloads & links

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Map

FileDownload / File: emd_13642.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.9 Å/pix.
x 360 pix.
= 324. Å		0.9 Å/pix.
x 360 pix.
= 324. Å		0.9 Å/pix.
x 360 pix.
= 324. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.9 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0135 / Movie #1: 0.0135
Minimum - Maximum-0.024193648 - 0.047737654
Average (Standard dev.)0.000054613232 (±0.00092841586)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 324.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.90.90.9
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z324.000324.000324.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.0240.0480.000

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Supplemental data

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Sample components

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Entire : Alpha-latrocrustotoxin-Lt1a in soluble monomeric state

EntireName: Alpha-latrocrustotoxin-Lt1a in soluble monomeric state
Components
  • Complex: Alpha-latrocrustotoxin-Lt1a in soluble monomeric state
    • Protein or peptide: Alpha-latrocrustotoxin-Lt1a

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Supramolecule #1: Alpha-latrocrustotoxin-Lt1a in soluble monomeric state

SupramoleculeName: Alpha-latrocrustotoxin-Lt1a in soluble monomeric state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Latrodectus tredecimguttatus (black widow)
Molecular weightTheoretical: 139.8 KDa

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Macromolecule #1: Alpha-latrocrustotoxin-Lt1a

MacromoleculeName: Alpha-latrocrustotoxin-Lt1a / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Latrodectus tredecimguttatus (black widow)
Molecular weightTheoretical: 140.010531 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MWSHPQFEKG SAGSAAGSGA GWSHPQFEKG AGLEVLFQGP NSEMKGKRVI SKREMSKADQ CTFLSYQSVA YGTLGDVAGD VSSIEGADL VATPIAAGGH LAKGATDAAM IAMDCSSIPF DEIKQQLNQR FNEVDKKLQK GAEALENVTE LAEKTYSSVE K MRVEMREG ...String:
MWSHPQFEKG SAGSAAGSGA GWSHPQFEKG AGLEVLFQGP NSEMKGKRVI SKREMSKADQ CTFLSYQSVA YGTLGDVAGD VSSIEGADL VATPIAAGGH LAKGATDAAM IAMDCSSIPF DEIKQQLNQR FNEVDKKLQK GAEALENVTE LAEKTYSSVE K MRVEMREG FNHVIATIEN ANTKQIITGI NQIIQYFNDE RENINNRQKE DYVAKLQEPA SGNFLLYLRK SRTSEDGSLH SL LFKIINQ ELAIPNNAAD NNAIRALFAL FYGTQTFISI MFYLVKQYSY LADYHYQNGN LAEFNSNFDH MKTVFQDFKF TLI GINTSN SKPLVNTVLS IIEDVKNKRF IRNLRSNLYQ KIIKSTKSLL DLREKITKMD LPIIEDTPKS SVLINFREKS SSVP RIETP ILKWTPGTVV KYAIQYEQDG KYSKISKWSN PITVQRLANP YITIDKDRRN RLVFRQFGNE KPELISILDS SQNEF RDIH RDLYNAAQMP YKETALGICR KLIDSGAQVG ASFEMGRKSI HASATAGNDD VARLLLAKNN GLLNVPDKNG YTPLHI ASE RKNNDFVKFL LEKGADVNVR TFANELTPLH LAARQDFTII VKTLMEKRGI DVNAKERAGF TPLHLSITSN SRAARTL IN ETPAGINIKS NSGLTPLHLA VLQNNLSAAK VLVKSNKKVK LNEMDNNGMT PLHYASMLGN LEFVKYFTSE QGIDVNAK T KVKNWTPLHL AILFKKFDVA QSLLQVRNID ISTRADQAIT PLHLAAATGN SQIVKTILNS GAVVDQETAN GFTALHLAI MNPNTETPQF LIAKGANINA KTNDGSTPLH FAAALGKTNI FQLLMDKGAN IKAENLINQM PIHEAVVNGH LAIVKMLIEQ DSSLMNAKN MRDEYPFYLA AEKRYKDVFN YLESKGADVN EKNNDGNTLL HLFSINGEVE VVQFLIQNGA DFRLRNKERK S FFDLAVEF GHAGIVGYAI EENKVDLQEP YRGKTILYHA ICDSVKYDRI EVVRYFVETL NEDQCSPLQE AAAYAHLDLV KY FVQERGI NPTAFNNDNQ VSPLCIAIVG APCGFVKSCD TPERLDVVEY LVDKTPDINK ECDTQQSTPV SSAVYGNKVS ILN YLIRNG ADPNKKVRGD PPLFIAAMIG QYDIVKSLVE QHKIDVNTRN KEQFTPLHAA ASNDHIDVVK YLIQKGADVN AKGD ENLKP IDLAGEKSKA YLRSLGRRFF RNESPSKSFE ISSGLVPRGS HHHHHHHH

UniProtKB: Alpha-latrocrustotoxin-Lt1a

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
100.0 mMTris-HCltrisaminomethane hydrochloride
150.0 mMNaClsodium chloride
1.0 mMEDTAEthylenediaminetetraacetic acid
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 1 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 69.1 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.03 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: SPHIRE (ver. 1.3) / Number images used: 376474
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationSoftware - Name: RELION (ver. 3.1)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-7ptx:
Alpha-latrocrustotoxin monomer

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