[English] 日本語
Yorodumi
- EMDB-12516: B-brick bare in 5 mM Mg2+ -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-12516
TitleB-brick bare in 5 mM Mg2+
Map dataComposite map from Multibody and PostProcessing job
Sample
  • Complex: B-brick bare in 5 mM Mg2+
    • DNA: x 79 types
Biological speciessynthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 10.38 Å
AuthorsBertosin E / Stoemmer P / Feigl E / Wenig M / Honemann M / Dietz H
Funding support Germany, 4 items
OrganizationGrant numberCountry
German Research Foundation (DFG)Gottfried-Wilhelm-Leibniz Program Germany
European Research Council (ERC)Consolidator Grant (GA #724261) Germany
Max Planck SocietyMax Planck School Matter to Life Germany
German Research Foundation (DFG)SFB863 TPA9 Project ID 111166240 Germany
CitationJournal: ACS Nano / Year: 2021
Title: Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures.
Authors: Eva Bertosin / Pierre Stömmer / Elija Feigl / Maximilian Wenig / Maximilian N Honemann / Hendrik Dietz /
Abstract: Cationic coatings can enhance the stability of synthetic DNA objects in low ionic strength environments such as physiological fluids. Here, we used single-particle cryo-electron microscopy (cryo-EM), ...Cationic coatings can enhance the stability of synthetic DNA objects in low ionic strength environments such as physiological fluids. Here, we used single-particle cryo-electron microscopy (cryo-EM), pseudoatomic model fitting, and single-molecule mass photometry to study oligolysine and polyethylene glycol (PEG)-oligolysine-coated multilayer DNA origami objects. The coatings preserve coarse structural features well on a resolution of multiple nanometers but can also induce deformations such as twisting and bending. Higher-density coatings also led to internal structural deformations in the DNA origami test objects, in which a designed honeycomb-type helical lattice was deformed into a more square-lattice-like pattern. Under physiological ionic strength, where the uncoated objects disassembled, the coated objects remained intact but they shrunk in the helical direction and expanded in the direction perpendicular to the helical axis. Helical details like major/minor grooves and crossover locations were not discernible in cryo-EM maps that we determined of DNA origami coated with oligolysine and PEG-oligolysine, whereas these features were visible in cryo-EM maps determined from the uncoated reference objects. Blunt-ended double-helical interfaces remained accessible underneath the coating and may be used for the formation of multimeric DNA origami assemblies that rely on stacking interactions between blunt-ended helices. The ionic strength requirements for forming multimers from coated DNA origami differed from those needed for uncoated objects. Using single-molecule mass photometry, we found that the mass of coated DNA origami objects prior to and after incubation in low ionic strength physiological conditions remained unchanged. This finding indicated that the coating effectively prevented strand dissociation but also that the coating itself remained stable in place. Our results validate oligolysine coatings as a powerful stabilization method for DNA origami but also reveal several potential points of failure that experimenters should watch to avoid working with false premises.
History
DepositionFeb 27, 2021-
Header (metadata) releaseMar 31, 2021-
Map releaseMar 31, 2021-
UpdateJul 7, 2021-
Current statusJul 7, 2021Processing site: PDBe / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.08
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7npn
  • Surface level: 0.08
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

-
Map

FileDownload / File: emd_12516.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map from Multibody and PostProcessing job
Voxel sizeX=Y=Z: 2.319 Å
Density
Contour LevelBy AUTHOR: 0.0628 / Movie #1: 0.08
Minimum - Maximum-0.06287109 - 0.37234735
Average (Standard dev.)0.0017090228 (±0.014890234)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-150-150-150
Dimensions300300300
Spacing300300300
CellA=B=C: 695.7 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.3192.3192.319
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z695.700695.700695.700
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-150-150-150
NC/NR/NS300300300
D min/max/mean-0.0630.3720.002

-
Supplemental data

-
Additional map: Refined and post-processed map

Fileemd_12516_additional_1.map
AnnotationRefined and post-processed map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

-
Sample components

+
Entire : B-brick bare in 5 mM Mg2+

EntireName: B-brick bare in 5 mM Mg2+
Components
  • Complex: B-brick bare in 5 mM Mg2+
    • DNA: SCAFFOLD STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND
    • DNA: STAPLE STRAND

+
Supramolecule #1: B-brick bare in 5 mM Mg2+

SupramoleculeName: B-brick bare in 5 mM Mg2+ / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: synthetic construct (others)

+
Macromolecule #1: SCAFFOLD STRAND

MacromoleculeName: SCAFFOLD STRAND / type: dna / ID: 1 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 887.0455 KDa
SequenceString: (DG)(DT)(DG)(DG)(DC)(DA)(DC)(DT)(DT)(DT) (DT)(DC)(DG)(DG)(DG)(DG)(DA)(DA)(DA)(DT) (DG)(DT)(DG)(DC)(DG)(DC)(DG)(DG)(DA) (DA)(DC)(DC)(DC)(DC)(DT)(DA)(DT)(DT)(DT) (DG) (DT)(DT)(DT)(DA)(DT)(DT) ...String:
(DG)(DT)(DG)(DG)(DC)(DA)(DC)(DT)(DT)(DT) (DT)(DC)(DG)(DG)(DG)(DG)(DA)(DA)(DA)(DT) (DG)(DT)(DG)(DC)(DG)(DC)(DG)(DG)(DA) (DA)(DC)(DC)(DC)(DC)(DT)(DA)(DT)(DT)(DT) (DG) (DT)(DT)(DT)(DA)(DT)(DT)(DT)(DT) (DT)(DC)(DT)(DA)(DA)(DA)(DT)(DA)(DC)(DA) (DT)(DT) (DC)(DA)(DA)(DA)(DT)(DA)(DT) (DG)(DT)(DA)(DT)(DC)(DC)(DG)(DC)(DT)(DC) (DA)(DT)(DG) (DA)(DG)(DA)(DC)(DA)(DA) (DT)(DA)(DA)(DC)(DC)(DC)(DT)(DG)(DA)(DT) (DA)(DA)(DA)(DT) (DG)(DC)(DT)(DT)(DC) (DA)(DA)(DT)(DA)(DA)(DT)(DA)(DT)(DT)(DG) (DA)(DA)(DA)(DA)(DA) (DG)(DG)(DA)(DA) (DG)(DA)(DG)(DT)(DA)(DT)(DG)(DA)(DG)(DT) (DA)(DT)(DT)(DC)(DA)(DA) (DC)(DA)(DT) (DT)(DT)(DC)(DC)(DG)(DT)(DG)(DT)(DC)(DG) (DC)(DC)(DC)(DT)(DT)(DA)(DT) (DT)(DC) (DC)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DG)(DC) (DG)(DG)(DC)(DA)(DT)(DT)(DT)(DT) (DG) (DC)(DC)(DT)(DT)(DC)(DC)(DT)(DG)(DT)(DT) (DT)(DT)(DT)(DG)(DC)(DT)(DC)(DA)(DC) (DC)(DC)(DA)(DG)(DA)(DA)(DA)(DC)(DG)(DC) (DT)(DG)(DG)(DT)(DG)(DA)(DA)(DA)(DG)(DT) (DA)(DA)(DA)(DA)(DG)(DA)(DT)(DG)(DC) (DT)(DG)(DA)(DA)(DG)(DA)(DT)(DC)(DA)(DG) (DT) (DT)(DG)(DG)(DG)(DT)(DG)(DC)(DA) (DC)(DG)(DA)(DG)(DT)(DG)(DG)(DG)(DT)(DT) (DA)(DC) (DA)(DT)(DC)(DG)(DA)(DA)(DC) (DT)(DG)(DG)(DA)(DT)(DC)(DT)(DC)(DA)(DA) (DC)(DA)(DG) (DC)(DG)(DG)(DT)(DA)(DA) (DG)(DA)(DT)(DC)(DC)(DT)(DT)(DG)(DA)(DG) (DA)(DG)(DT)(DT) (DT)(DT)(DC)(DG)(DC) (DC)(DC)(DC)(DG)(DA)(DA)(DG)(DA)(DA)(DC) (DG)(DT)(DT)(DT)(DT) (DC)(DC)(DA)(DA) (DT)(DG)(DA)(DT)(DG)(DA)(DG)(DC)(DA)(DC) (DT)(DT)(DT)(DT)(DA)(DA) (DA)(DG)(DT) (DT)(DC)(DT)(DG)(DC)(DT)(DA)(DT)(DG)(DT) (DG)(DG)(DC)(DG)(DC)(DG)(DG) (DT)(DA) (DT)(DT)(DA)(DT)(DC)(DC)(DC)(DG)(DT)(DA) (DT)(DT)(DG)(DA)(DC)(DG)(DC)(DC) (DG) (DG)(DG)(DC)(DA)(DA)(DG)(DA)(DG)(DC)(DA) (DA)(DC)(DT)(DC)(DG)(DG)(DT)(DC)(DG) (DC)(DC)(DG)(DC)(DA)(DT)(DA)(DC)(DA)(DC) (DT)(DA)(DT)(DT)(DC)(DT)(DC)(DA)(DG)(DA) (DA)(DT)(DG)(DA)(DC)(DT)(DT)(DG)(DG) (DT)(DT)(DG)(DA)(DG)(DT)(DA)(DC)(DT)(DC) (DA) (DC)(DC)(DA)(DG)(DT)(DC)(DA)(DC) (DA)(DG)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DT) (DC)(DT) (DT)(DA)(DC)(DG)(DG)(DA)(DT) (DG)(DG)(DC)(DA)(DT)(DG)(DA)(DC)(DA)(DG) (DT)(DA)(DA) (DG)(DA)(DG)(DA)(DA)(DT) (DT)(DA)(DT)(DG)(DC)(DA)(DG)(DT)(DG)(DC) (DT)(DG)(DC)(DC) (DA)(DT)(DA)(DA)(DC) (DC)(DA)(DT)(DG)(DA)(DG)(DT)(DG)(DA)(DT) (DA)(DA)(DC)(DA)(DC) (DT)(DG)(DC)(DG) (DG)(DC)(DC)(DA)(DA)(DC)(DT)(DT)(DA)(DC) (DT)(DT)(DC)(DT)(DG)(DA) (DC)(DA)(DA) (DC)(DG)(DA)(DT)(DC)(DG)(DG)(DA)(DG)(DG) (DA)(DC)(DC)(DG)(DA)(DA)(DG) (DG)(DA) (DG)(DC)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DT) (DT)(DT)(DT)(DT)(DT)(DG)(DC)(DA) (DC) (DA)(DA)(DC)(DA)(DT)(DG)(DG)(DG)(DG)(DG) (DA)(DT)(DC)(DA)(DT)(DG)(DT)(DA)(DA) (DC)(DT)(DC)(DG)(DC)(DC)(DT)(DT)(DG)(DA) (DT)(DC)(DG)(DT)(DT)(DG)(DG)(DG)(DA)(DA) (DC)(DC)(DG)(DG)(DA)(DG)(DC)(DT)(DG) (DA)(DA)(DT)(DG)(DA)(DA)(DG)(DC)(DC)(DA) (DT) (DA)(DC)(DC)(DA)(DA)(DA)(DC)(DG) (DA)(DC)(DG)(DA)(DG)(DC)(DG)(DT)(DG)(DA) (DC)(DA) (DC)(DC)(DA)(DC)(DG)(DA)(DT) (DG)(DC)(DC)(DT)(DG)(DT)(DA)(DG)(DC)(DA) (DA)(DT)(DG) (DG)(DC)(DA)(DA)(DC)(DA) (DA)(DC)(DG)(DT)(DT)(DG)(DC)(DG)(DC)(DA) (DA)(DA)(DC)(DT) (DA)(DT)(DT)(DA)(DA) (DC)(DT)(DG)(DG)(DC)(DG)(DA)(DA)(DC)(DT) (DA)(DC)(DT)(DT)(DA) (DC)(DT)(DC)(DT) (DA)(DG)(DC)(DT)(DT)(DC)(DC)(DC)(DG)(DG) (DC)(DA)(DA)(DC)(DA)(DA) (DT)(DT)(DA) (DA)(DT)(DA)(DG)(DA)(DC)(DT)(DG)(DG)(DA) (DT)(DG)(DG)(DA)(DG)(DG)(DC) (DG)(DG) (DA)(DT)(DA)(DA)(DA)(DG)(DT)(DT)(DG)(DC) (DA)(DG)(DG)(DA)(DC)(DC)(DA)(DC) (DT) (DT)(DC)(DT)(DG)(DC)(DG)(DC)(DT)(DC)(DG) (DG)(DC)(DC)(DC)(DT)(DT)(DC)(DC)(DG) (DG)(DC)(DT)(DG)(DG)(DC)(DT)(DG)(DG)(DT) (DT)(DT)(DA)(DT)(DT)(DG)(DC)(DT)(DG)(DA) (DT)(DA)(DA)(DA)(DT)(DC)(DT)(DG)(DG) (DA)(DG)(DC)(DC)(DG)(DG)(DT)(DG)(DA)(DG) (DC) (DG)(DT)(DG)(DG)(DG)(DT)(DC)(DA) (DC)(DG)(DC)(DG)(DG)(DT)(DA)(DT)(DC)(DA) (DT)(DT) (DG)(DC)(DA)(DG)(DC)(DA)(DC) (DT)(DG)(DG)(DG)(DG)(DC)(DC)(DA)(DG)(DA) (DT)(DG)(DG) (DT)(DA)(DA)(DG)(DC)(DC) (DC)(DT)(DC)(DC)(DC)(DG)(DT)(DA)(DT)(DC) (DG)(DT)(DA)(DG) (DT)(DT)(DA)(DT)(DC) (DT)(DA)(DC)(DA)(DC)(DG)(DA)(DC)(DG)(DG) (DG)(DG)(DA)(DG)(DT) (DC)(DA)(DG)(DG) (DC)(DA)(DA)(DC)(DT)(DA)(DT)(DG)(DG)(DA) (DT)(DG)(DA)(DA)(DC)(DG) (DA)(DA)(DA) (DT)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DT)(DC) (DG)(DC)(DT)(DG)(DA)(DG)(DA) (DT)(DA) (DG)(DG)(DT)(DG)(DC)(DC)(DT)(DC)(DA)(DC) (DT)(DG)(DA)(DT)(DT)(DA)(DA)(DG) (DC) (DA)(DT)(DT)(DG)(DG)(DT)(DA)(DA)(DC)(DT) (DG)(DT)(DC)(DA)(DG)(DA)(DC)(DC)(DA) (DA)(DG)(DT)(DT)(DT)(DA)(DC)(DT)(DC)(DA) (DT)(DA)(DT)(DA)(DT)(DA)(DC)(DT)(DT)(DT) (DA)(DG)(DA)(DT)(DT)(DG)(DA)(DT)(DT) (DT)(DA)(DA)(DA)(DA)(DC)(DT)(DT)(DC)(DA) (DT) (DT)(DT)(DT)(DT)(DA)(DA)(DT)(DT) (DT)(DA)(DA)(DA)(DA)(DG)(DG)(DA)(DT)(DC) (DT)(DA) (DG)(DG)(DT)(DG)(DA)(DA)(DG) (DA)(DT)(DC)(DC)(DT)(DT)(DT)(DT)(DT)(DG) (DA)(DT)(DA) (DA)(DT)(DC)(DT)(DC)(DA) (DT)(DG)(DA)(DC)(DC)(DA)(DA)(DA)(DA)(DT) (DC)(DC)(DC)(DT) (DT)(DA)(DA)(DC)(DG) (DT)(DG)(DA)(DG)(DT)(DT)(DT)(DT)(DC)(DG) (DT)(DT)(DC)(DC)(DA) (DC)(DT)(DG)(DA) (DG)(DC)(DG)(DT)(DC)(DA)(DG)(DA)(DC)(DC) (DC)(DC)(DG)(DT)(DA)(DG) (DA)(DA)(DA) (DA)(DG)(DA)(DT)(DC)(DA)(DA)(DA)(DG)(DG) (DA)(DT)(DC)(DT)(DT)(DC)(DT) (DT)(DG) (DA)(DG)(DA)(DT)(DC)(DC)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DC)(DT)(DG)(DC)(DG) (DC) (DG)(DT)(DA)(DA)(DT)(DC)(DT)(DG)(DC)(DT) (DG)(DC)(DT)(DT)(DG)(DC)(DA)(DA)(DA) (DC)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DC) (DA)(DC)(DC)(DG)(DC)(DT)(DA)(DC)(DC)(DA) (DG)(DC)(DG)(DG)(DT)(DG)(DG)(DT)(DT) (DT)(DG)(DT)(DT)(DT)(DG)(DC)(DC)(DG)(DG) (DA) (DT)(DC)(DA)(DA)(DG)(DA)(DG)(DC) (DT)(DA)(DC)(DC)(DA)(DA)(DC)(DT)(DC)(DT) (DT)(DT) (DT)(DT)(DC)(DC)(DG)(DA)(DA) (DG)(DG)(DT)(DA)(DA)(DC)(DT)(DG)(DG)(DC) (DT)(DT)(DC) (DA)(DG)(DC)(DA)(DG)(DA) (DG)(DC)(DG)(DC)(DA)(DG)(DA)(DT)(DA)(DC) (DC)(DA)(DA)(DA) (DT)(DA)(DC)(DT)(DG) (DT)(DT)(DC)(DT)(DT)(DC)(DT)(DA)(DG)(DT) (DG)(DT)(DA)(DG)(DC) (DC)(DG)(DT)(DA) (DG)(DT)(DT)(DA)(DG)(DG)(DC)(DC)(DA)(DC) (DC)(DA)(DC)(DT)(DT)(DC) (DA)(DA)(DG) (DA)(DA)(DC)(DT)(DC)(DT)(DG)(DT)(DA)(DG) (DC)(DA)(DC)(DC)(DG)(DC)(DC) (DT)(DA) (DC)(DA)(DT)(DA)(DC)(DC)(DT)(DC)(DG)(DC) (DT)(DC)(DT)(DG)(DC)(DT)(DA)(DA) (DT) (DC)(DC)(DT)(DG)(DT)(DT)(DA)(DC)(DC)(DA) (DG)(DT)(DG)(DG)(DC)(DT)(DG)(DC)(DT) (DG)(DC)(DC)(DA)(DG)(DT)(DG)(DG)(DC)(DG) (DA)(DT)(DA)(DA)(DG)(DT)(DC)(DG)(DT)(DG) (DT)(DC)(DT)(DT)(DA)(DC)(DC)(DG)(DG) (DG)(DT)(DT)(DG)(DG)(DA)(DC)(DT)(DC)(DA) (DA) (DG)(DA)(DC)(DG)(DA)(DT)(DA)(DG) (DT)(DT)(DA)(DC)(DC)(DG)(DG)(DA)(DT)(DA) (DA)(DG) (DG)(DC)(DG)(DC)(DA)(DG)(DC) (DG)(DG)(DT)(DC)(DG)(DG)(DG)(DC)(DT)(DG) (DA)(DA)(DC) (DG)(DG)(DG)(DG)(DG)(DG) (DT)(DT)(DC)(DG)(DT)(DG)(DC)(DA)(DC)(DA) (DC)(DA)(DG)(DC) (DC)(DC)(DA)(DG)(DC) (DT)(DT)(DG)(DG)(DA)(DG)(DC)(DG)(DA)(DA) (DC)(DG)(DA)(DC)(DC) (DT)(DA)(DC)(DA) (DC)(DC)(DG)(DA)(DA)(DC)(DT)(DG)(DA)(DG) (DA)(DT)(DA)(DC)(DC)(DT) (DA)(DC)(DA) (DG)(DC)(DG)(DT)(DG)(DA)(DG)(DC)(DT)(DA) (DT)(DG)(DA)(DG)(DA)(DA)(DA) (DG)(DC) (DG)(DC)(DC)(DA)(DC)(DG)(DC)(DT)(DT)(DC) (DC)(DC)(DG)(DA)(DA)(DG)(DG)(DG) (DA) (DG)(DA)(DA)(DA)(DG)(DG)(DC)(DG)(DG)(DA) (DC)(DA)(DG)(DG)(DT)(DA)(DT)(DC)(DC) (DG)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DC) (DA)(DG)(DG)(DG)(DT)(DC)(DG)(DG)(DA)(DA) (DC)(DA)(DG)(DG)(DA)(DG)(DA)(DG)(DC) (DG)(DC)(DA)(DC)(DG)(DA)(DG)(DG)(DG)(DA) (DG) (DC)(DT)(DT)(DC)(DC)(DA)(DG)(DG) (DG)(DG)(DG)(DA)(DA)(DA)(DC)(DG)(DC)(DC) (DT)(DG) (DG)(DT)(DA)(DT)(DC)(DT)(DT) (DT)(DA)(DT)(DA)(DG)(DT)(DC)(DC)(DT)(DG) (DT)(DC)(DG) (DG)(DG)(DT)(DT)(DT)(DC) (DG)(DC)(DC)(DA)(DC)(DC)(DT)(DC)(DT)(DG) (DA)(DC)(DT)(DT) (DG)(DA)(DG)(DC)(DG) (DT)(DC)(DG)(DA)(DT)(DT)(DT)(DT)(DT)(DG) (DT)(DG)(DA)(DT)(DG) (DC)(DT)(DC)(DG) (DT)(DC)(DA)(DG)(DG)(DG)(DG)(DG)(DG)(DC) (DG)(DG)(DA)(DG)(DC)(DC) (DT)(DA)(DT) (DG)(DG)(DA)(DA)(DA)(DA)(DA)(DC)(DG)(DC) (DC)(DA)(DG)(DC)(DA)(DA)(DC) (DG)(DC) (DG)(DG)(DC)(DC)(DT)(DT)(DT)(DT)(DT)(DA) (DC)(DG)(DG)(DT)(DT)(DC)(DC)(DT) (DG) (DG)(DC)(DC)(DT)(DT)(DT)(DT)(DG)(DC)(DT) (DG)(DG)(DC)(DC)(DT)(DT)(DT)(DT)(DG) (DC)(DT)(DC)(DA)(DC)(DA)(DT)(DG)(DT)(DT) (DC)(DT)(DT)(DT)(DC)(DC)(DT)(DG)(DC)(DG) (DT)(DT)(DA)(DT)(DC)(DC)(DC)(DC)(DT) (DG)(DA)(DT)(DT)(DC)(DT)(DG)(DT)(DG)(DG) (DA) (DT)(DA)(DA)(DC)(DC)(DG)(DT)(DA) (DT)(DT)(DA)(DC)(DC)(DG)(DC)(DC)(DT)(DT) (DT)(DG) (DA)(DG)(DT)(DG)(DA)(DG)(DC) (DT)(DG)(DA)(DT)(DA)(DC)(DC)(DG)(DC)(DT) (DC)(DG)(DC) (DC)(DG)(DC)(DA)(DG)(DC) (DC)(DG)(DA)(DA)(DC)(DG)(DA)(DC)(DC)(DG) (DA)(DG)(DC)(DG) (DC)(DA)(DG)(DC)(DG) (DA)(DG)(DT)(DC)(DA)(DG)(DT)(DG)(DA)(DG) (DC)(DG)(DA)(DG)(DG) (DA)(DA)(DG)(DC) (DG)(DG)(DA)(DA)(DG)(DA)(DG)(DC)(DG)(DC) (DC)(DC)(DA)(DA)(DT)(DA) (DC)(DG)(DC) (DA)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DT)(DC) (DT)(DC)(DC)(DC)(DC)(DG)(DC) (DG)(DC) (DG)(DT)(DT)(DG)(DG)(DC)(DC)(DG)(DA)(DT) (DT)(DC)(DA)(DT)(DT)(DA)(DA)(DT) (DG) (DC)(DA)(DG)(DC)(DT)(DG)(DG)(DC)(DA)(DC) (DG)(DA)(DC)(DA)(DG)(DG)(DT)(DT)(DT) (DC)(DC)(DC)(DG)(DA)(DC)(DT)(DG)(DG)(DA) (DA)(DA)(DG)(DC)(DG)(DG)(DG)(DC)(DA)(DG) (DT)(DG)(DA)(DG)(DC)(DG)(DC)(DA)(DA) (DC)(DG)(DC)(DA)(DA)(DT)(DT)(DA)(DA)(DT) (DG) (DT)(DG)(DA)(DG)(DT)(DT)(DA)(DG) (DC)(DT)(DC)(DA)(DC)(DT)(DC)(DA)(DT)(DT) (DA)(DG) (DG)(DC)(DA)(DC)(DC)(DC)(DC) (DA)(DG)(DG)(DC)(DT)(DT)(DT)(DA)(DC)(DA) (DC)(DT)(DT) (DT)(DA)(DT)(DG)(DC)(DT) (DT)(DC)(DC)(DG)(DG)(DC)(DT)(DC)(DG)(DT) (DA)(DT)(DG)(DT) (DT)(DG)(DT)(DG)(DT) (DG)(DG)(DA)(DA)(DT)(DT)(DG)(DT)(DG)(DA) (DG)(DC)(DG)(DG)(DA) (DT)(DA)(DA)(DC) (DA)(DA)(DT)(DT)(DT)(DC)(DA)(DC)(DA)(DC) (DA)(DG)(DG)(DA)(DA)(DA) (DC)(DA)(DG) (DC)(DT)(DA)(DT)(DG)(DA)(DC)(DC)(DA)(DT) (DG)(DA)(DT)(DT)(DA)(DC)(DG) (DC)(DC) (DA)(DA)(DG)(DC)(DG)(DC)(DG)(DA)(DA)(DT) (DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG) (DC) (DT)(DT)(DA)(DG)(DA)(DA)(DT)(DT)(DC)(DG) (DC)(DT)(DC)(DA)(DC)(DT)(DG)(DG)(DC) (DC)(DG)(DT)(DC)(DG)(DT)(DT)(DT)(DT)(DA) (DC)(DA)(DC)(DC)(DA)(DT)(DG)(DA)(DT)(DT) (DA)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DC) (DG)(DC)(DG)(DA)(DA)(DT)(DT)(DC)(DT)(DC) (DC) (DA)(DG)(DG)(DC)(DT)(DT)(DA)(DG) (DA)(DA)(DT)(DT)(DC)(DG)(DC)(DT)(DC)(DA) (DC)(DT) (DG)(DG)(DC)(DC)(DG)(DT)(DC) (DG)(DT)(DT)(DT)(DT)(DA)(DC)(DA)(DA)(DC) (DG)(DT)(DC) (DG)(DT)(DG)(DA)(DC)(DT) (DG)(DG)(DG)(DA)(DA)(DA)(DA)(DC)(DC)(DC) (DT)(DG)(DG)(DC) (DG)(DT)(DT)(DA)(DC) (DC)(DC)(DA)(DA)(DC)(DT)(DT)(DA)(DA)(DT) (DC)(DG)(DC)(DC)(DT) (DT)(DG)(DC)(DA) (DG)(DC)(DA)(DC)(DA)(DT)(DC)(DC)(DC)(DC) (DC)(DT)(DT)(DT)(DC)(DG) (DC)(DC)(DA) (DG)(DC)(DT)(DG)(DG)(DC)(DG)(DT)(DA)(DA) (DT)(DA)(DG)(DC)(DG)(DA)(DA) (DG)(DA) (DG)(DG)(DC)(DC)(DC)(DG)(DC)(DA)(DC)(DC) (DG)(DA)(DT)(DC)(DG)(DC)(DC)(DC) (DT) (DT)(DC)(DC)(DC)(DA)(DA)(DC)(DA)(DG)(DT) (DT)(DG)(DC)(DG)(DC)(DA)(DG)(DC)(DC) (DT)(DG)(DA)(DA)(DT)(DG)(DG)(DC)(DG)(DA) (DA)(DT)(DG)(DG)(DG)(DA)(DC)(DG)(DC)(DG) (DC)(DC)(DC)(DT)(DG)(DT)(DA)(DG)(DC) (DG)(DG)(DC)(DG)(DC)(DA)(DT)(DT)(DA)(DA) (DG) (DC)(DG)(DC)(DG)(DG)(DC)(DG)(DG) (DG)(DT)(DG)(DT)(DG)(DG)(DT)(DG)(DG)(DT) (DT)(DA) (DC)(DG)(DC)(DG)(DC)(DA)(DG) (DC)(DG)(DT)(DG)(DA)(DC)(DC)(DG)(DC)(DT) (DA)(DC)(DA) (DC)(DT)(DT)(DG)(DC)(DC) (DA)(DG)(DC)(DG)(DC)(DC)(DC)(DT)(DA)(DG) (DC)(DG)(DC)(DC) (DC)(DG)(DC)(DT)(DC) (DC)(DT)(DT)(DT)(DC)(DG)(DC)(DT)(DT)(DT) (DC)(DT)(DT)(DC)(DC) (DC)(DT)(DT)(DC) (DC)(DT)(DT)(DT)(DC)(DT)(DC)(DG)(DC)(DC) (DA)(DC)(DG)(DT)(DT)(DC) (DG)(DC)(DC) (DG)(DG)(DC)(DT)(DT)(DT)(DC)(DC)(DC)(DC) (DG)(DT)(DC)(DA)(DA)(DG)(DC) (DT)(DC) (DT)(DA)(DA)(DA)(DT)(DC)(DG)(DG)(DG)(DG) (DG)(DC)(DT)(DC)(DC)(DC)(DT)(DT) (DT) (DA)(DG)(DG)(DG)(DT)(DT)(DC)(DC)(DG)(DA) (DT)(DT)(DT)(DA)(DG)(DT)(DG)(DC)(DT) (DT)(DT)(DA)(DC)(DG)(DG)(DC)(DA)(DC)(DC) (DT)(DC)(DG)(DA)(DC)(DC)(DC)(DC)(DA)(DA) (DA)(DA)(DA)(DA)(DC)(DT)(DT)(DG)(DA) (DT)(DT)(DA)(DG)(DG)(DG)(DT)(DG)(DA)(DT) (DG) (DG)(DT)(DT)(DC)(DA)(DC)(DG)(DT) (DA)(DG)(DT)(DG)(DG)(DG)(DC)(DC)(DA)(DT) (DC)(DG) (DC)(DC)(DC)(DT)(DG)(DA)(DT) (DA)(DG)(DA)(DC)(DG)(DG)(DT)(DT)(DT)(DT) (DT)(DC)(DG) (DC)(DC)(DC)(DT)(DT)(DT) (DG)(DA)(DC)(DG)(DT)(DT)(DG)(DG)(DA)(DG) (DT)(DC)(DC)(DA) (DC)(DG)(DT)(DT)(DC) (DT)(DT)(DT)(DA)(DA)(DT)(DA)(DG)(DT)(DG) (DG)(DA)(DC)(DT)(DC) (DT)(DT)(DG)(DT) (DT)(DC)(DC)(DA)(DA)(DA)(DC)(DT)(DG)(DG) (DA)(DA)(DC)(DA)(DA)(DC) (DA)(DC)(DT) (DC)(DA)(DA)(DC)(DC)(DC)(DT)(DA)(DT)(DC) (DT)(DC)(DG)(DG)(DT)(DC)(DT) (DA)(DT) (DT)(DC)(DT)(DT)(DT)(DT)(DG)(DA)(DT)(DT) (DT)(DA)(DT)(DA)(DA)(DG)(DG)(DG) (DA) (DT)(DT)(DT)(DT)(DG)(DC)(DC)(DG)(DA)(DT) (DT)(DT)(DC)(DG)(DG)(DC)(DC)(DT)(DA) (DT)(DT)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA) (DA)(DA)(DT)(DG)(DA)(DG)(DC)(DT)(DG)(DA) (DT)(DT)(DT)(DA)(DA)(DC)(DA)(DA)(DA) (DA)(DA)(DT)(DT)(DT)(DA)(DA)(DC)(DG)(DC) (DG) (DA)(DA)(DT)(DT)(DT)(DT)(DA)(DA) (DC)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DT)(DA) (DA)(DC) (DG)(DC)(DT)(DT)(DA)(DC)(DA) (DA)(DT)(DT)(DT)(DA)(DG)

+
Macromolecule #2: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.418716 KDa
SequenceString:
(DT)(DT)(DC)(DT)(DG)(DA)(DA)(DA)(DC)(DC) (DC)(DG)(DA)(DC)(DA)(DG)(DG)(DA)(DC)(DT) (DT)(DC)(DA)(DA)(DG)(DT)(DC)(DG)(DG) (DT)(DA)(DT)(DC)(DT)

+
Macromolecule #3: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 14.620335 KDa
SequenceString:
(DT)(DG)(DG)(DC)(DG)(DT)(DT)(DT)(DC)(DC) (DC)(DC)(DC)(DT)(DG)(DG)(DA)(DA)(DG)(DC) (DT)(DT)(DT)(DT)(DT)(DG)(DT)(DT)(DC) (DT)(DG)(DC)(DG)(DC)(DT)(DA)(DC)(DA)(DC) (DT) (DA)(DC)(DG)(DC)(DC)(DA)(DC)(DT)

+
Macromolecule #4: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.55385 KDa
SequenceString:
(DT)(DA)(DG)(DG)(DG)(DA)(DA)(DG)(DA)(DT) (DC)(DC)(DT)(DT)(DT)(DG)(DA)(DT)(DC)(DT) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DA) (DT)(DG)(DA)(DG)(DT)

+
Macromolecule #5: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 5 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.62612 KDa
SequenceString:
(DT)(DC)(DT)(DG)(DA)(DC)(DG)(DC)(DT)(DC) (DA)(DG)(DT)(DG)(DG)(DA)(DA)(DC)(DG)(DA) (DT)(DT)(DG)(DC)(DC)(DA)(DT)(DA)(DA) (DG)(DC)(DT)(DA)(DG)(DA)(DT)(DC)(DC)(DA) (DG) (DT)

+
Macromolecule #6: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 6 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.544041 KDa
SequenceString:
(DT)(DC)(DA)(DA)(DG)(DC)(DT)(DT)(DC)(DA) (DT)(DT)(DC)(DA)(DG)(DC)(DT)(DC)(DC)(DG) (DC)(DT)(DC)(DG)(DT)(DC)(DG)(DA)(DA) (DG)(DT)(DT)(DG)(DG)(DC)(DT)(DG)(DC)(DA) (DT) (DA)

+
Macromolecule #7: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 7 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.384688 KDa
SequenceString:
(DT)(DC)(DA)(DA)(DG)(DG)(DC)(DG)(DA)(DG) (DT)(DT)(DA)(DC)(DA)(DT)(DG)(DA)(DT)(DC) (DT)(DC)(DT)(DT)(DA)(DC)(DC)(DT)(DG) (DC)(DT)(DC)(DA)(DT)

+
Macromolecule #8: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 8 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.936256 KDa
SequenceString:
(DA)(DG)(DA)(DG)(DG)(DT)(DG)(DG)(DC)(DC) (DC)(DT)(DG)(DT)(DT)(DC)(DC)(DG)(DA)(DC) (DC)(DT)(DT)(DT)(DC)(DT)(DC)(DA)(DG) (DG)(DC)(DT)(DG)(DT)(DG)(DT)(DC)(DT)(DT) (DG) (DA)(DG)

+
Macromolecule #9: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 9 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.653603 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DA)(DA) (DA)(DA)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DG) (DT)(DG)(DC)(DA)(DA)(DA)(DA)(DG)

+
Macromolecule #10: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 10 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.225543 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DT)(DT)(DC) (DT)(DC)(DC)(DC)(DT)(DT)(DC)(DG)(DT)(DT) (DC)(DA)(DG)(DC)(DC)(DC)(DG)(DA)(DC) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #11: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 11 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.950333 KDa
SequenceString:
(DC)(DA)(DG)(DT)(DT)(DA)(DT)(DT)(DT)(DT) (DG)(DG)(DT)(DA)(DT)(DT)(DG)(DC)(DA)(DA) (DG)(DC)(DA)(DG)(DC)(DA)(DT)(DC)(DA) (DT)(DG)(DG)(DT)(DG)(DT)(DT)(DC)(DA)(DC) (DA) (DA)(DT)

+
Macromolecule #12: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 12 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.027347 KDa
SequenceString:
(DA)(DA)(DG)(DG)(DC)(DC)(DA)(DT)(DG)(DC) (DT)(DG)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DT) (DC)(DC)(DA)(DT)(DA)(DG)(DG)(DC)(DG) (DA)(DA)(DC)(DA)(DT)(DG)(DG)(DA)(DG)(DC) (DG) (DG)(DT)

+
Macromolecule #13: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 13 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.010361 KDa
SequenceString:
(DA)(DA)(DT)(DA)(DC)(DG)(DG)(DT)(DT)(DC) (DG)(DG)(DT)(DG)(DT)(DA)(DG)(DG)(DT)(DC) (DG)(DA)(DC)(DA)(DC)(DG)(DA)(DC)(DT) (DT)(DT)(DA)(DA)(DT)(DG)(DA)(DA)(DT)(DC) (DG) (DG)(DC)

+
Macromolecule #14: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.171429 KDa
SequenceString:
(DA)(DG)(DG)(DC)(DG)(DG)(DT)(DC)(DA)(DA) (DC)(DG)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DA) (DG)(DC)(DG)(DG)(DG)(DA)(DA)(DA)(DC) (DC)(DG)(DG)(DA)(DA)(DG)(DA)(DG)(DC)(DT) (DG) (DG)(DC)

+
Macromolecule #15: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 15 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.156733 KDa
SequenceString:
(DC)(DA)(DC)(DT)(DC)(DA)(DA)(DG)(DA)(DT) (DA)(DA)(DC)(DG)(DT)(DA)(DT)(DC)(DC)(DG) (DC)(DA)(DA)(DA)(DA)(DC)(DG)(DA)(DT) (DT)(DC)(DG)(DC)(DG)(DC)(DT)(DT)(DG)(DG) (DC) (DG)(DT)(DA)(DG)(DG)(DG)(DG)(DG) (DA)

+
Macromolecule #16: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.062678 KDa
SequenceString:
(DC)(DT)(DG)(DC)(DG)(DG)(DC)(DT)(DG)(DA) (DG)(DC)(DA)(DA)(DC)(DT)(DG)(DT)(DT)(DT) (DC)(DA)(DT)(DT)(DC)(DT)(DA)(DA)(DG) (DC)(DG)(DA)(DA)(DT)(DT)(DC)(DT)(DA)(DA) (DG) (DC)(DC)(DA)(DT)(DT)(DA)(DA)(DG) (DT)

+
Macromolecule #17: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 17 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.496434 KDa
SequenceString:
(DG)(DC)(DT)(DC)(DG)(DG)(DT)(DG)(DC)(DT) (DT)(DC)(DC)(DT)(DC)(DG)(DC)(DT)(DC)(DA) (DC)(DT)(DG)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #18: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 18 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.528485 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DA)(DC)(DT)(DC)(DG) (DC)(DT)(DG)(DC)(DG)(DC)(DC)(DA)(DG)(DG) (DA)(DA)(DC)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #19: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 19 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.940277 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DC)(DT)(DG) (DC)(DG)(DC)(DC)(DT)(DT)(DA)(DT)(DC)(DC) (DG)(DG)(DT)(DA)(DA)(DG)(DA)(DG)(DC) (DG)(DA)(DG)(DG)(DT)(DA)(DT)(DG)(DT)(DT) (DT) (DT)(DT)

+
Macromolecule #20: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 20 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.84215 KDa
SequenceString: (DT)(DC)(DC)(DA)(DA)(DC)(DC)(DG)(DC)(DC) (DA)(DC)(DT)(DG)(DG)(DG)(DC)(DC)(DT)(DA) (DA)(DC)(DT)(DC)(DA)(DG)(DC)(DG)(DC) (DA)(DA)(DT)(DC)(DT)(DA)(DA)(DG)(DT)(DT) (DC) (DG)(DC)(DC)(DA)(DG)(DT) ...String:
(DT)(DC)(DC)(DA)(DA)(DC)(DC)(DG)(DC)(DC) (DA)(DC)(DT)(DG)(DG)(DG)(DC)(DC)(DT)(DA) (DA)(DC)(DT)(DC)(DA)(DG)(DC)(DG)(DC) (DA)(DA)(DT)(DC)(DT)(DA)(DA)(DG)(DT)(DT) (DC) (DG)(DC)(DC)(DA)(DG)(DT)(DT)(DT) (DT)(DT)(DT)(DT)

+
Macromolecule #21: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 21 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.23371 KDa
SequenceString:
(DT)(DT)(DG)(DC)(DG)(DT)(DA)(DT)(DT)(DG) (DG)(DG)(DC)(DG)(DG)(DC)(DT)(DC)(DA)(DC) (DT)(DC)(DC)(DT)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DC)(DC)(DT)(DC)(DT)(DG)(DG)(DG) (DT) (DA)(DA)(DC)(DG)(DT)(DA)(DA)(DA) (DG)

+
Macromolecule #22: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 22 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 16.03627 KDa
SequenceString: (DG)(DA)(DT)(DT)(DA)(DG)(DC)(DA)(DC)(DT) (DA)(DT)(DC)(DG)(DT)(DG)(DC)(DA)(DC)(DG) (DA)(DA)(DC)(DC)(DC)(DC)(DC)(DC)(DG) (DG)(DG)(DA)(DA)(DG)(DC)(DA)(DG)(DA)(DG) (DT) (DT)(DG)(DG)(DT)(DA)(DG) ...String:
(DG)(DA)(DT)(DT)(DA)(DG)(DC)(DA)(DC)(DT) (DA)(DT)(DC)(DG)(DT)(DG)(DC)(DA)(DC)(DG) (DA)(DA)(DC)(DC)(DC)(DC)(DC)(DC)(DG) (DG)(DG)(DA)(DA)(DG)(DC)(DA)(DG)(DA)(DG) (DT) (DT)(DG)(DG)(DT)(DA)(DG)(DC)(DT) (DT)(DT)(DT)(DT)

+
Macromolecule #23: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 23 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.950316 KDa
SequenceString:
(DG)(DG)(DC)(DA)(DG)(DC)(DA)(DC)(DG)(DG) (DT)(DA)(DA)(DG)(DT)(DT)(DC)(DG)(DC)(DT) (DC)(DC)(DA)(DA)(DG)(DC)(DT)(DG)(DT) (DA)(DG)(DC)(DT)(DC)(DA)(DA)(DA)(DG)(DC) (DC) (DA)(DG)

+
Macromolecule #24: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 24 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 11.535382 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DC)(DA)(DC) (DA)(DT)(DT)(DA)(DA)(DT)(DT)(DG)(DC)(DG) (DT)(DT)(DG)(DC)(DC)(DT)(DC)(DT)(DT) (DC)(DC)(DC)(DG)(DT)(DT)(DC)(DG)(DG)

+
Macromolecule #25: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 25 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 9.47613 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DG)(DT)(DG) (DC)(DT)(DA)(DC)(DA)(DG)(DA)(DT)(DT)(DC) (DA)(DT)(DC)(DC)(DA)(DT)(DT)(DA)(DA) (DA)(DA)

+
Macromolecule #26: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 26 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.065694 KDa
SequenceString:
(DA)(DT)(DA)(DC)(DG)(DA)(DG)(DC)(DC)(DT) (DG)(DT)(DC)(DG)(DT)(DG)(DC)(DC)(DA)(DG) (DC)(DT)(DG)(DC)(DA)(DT)(DA)(DT)(DG) (DA)(DA)(DG)(DA)(DA)(DC)(DC)(DT)(DT)(DA) (DA) (DT)(DC)(DA)(DA)(DA)(DC)(DT)(DT) (DG)

+
Macromolecule #27: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 27 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.518132 KDa
SequenceString:
(DT)(DC)(DC)(DA)(DC)(DT)(DA)(DC)(DC)(DA) (DC)(DA)(DG)(DA)(DA)(DC)(DA)(DA)(DA)(DA) (DA)(DT)(DC)(DG)(DA)(DC)(DG)(DC)(DA) (DT)(DA)(DA)(DA)(DG)(DA)(DT)(DA)(DC)(DC) (DA) (DT)

+
Macromolecule #28: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 28 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.894291 KDa
SequenceString:
(DA)(DA)(DA)(DT)(DT)(DG)(DT)(DC)(DA)(DG) (DG)(DA)(DA)(DA)(DT)(DC)(DC)(DG)(DC)(DC) (DC)(DC)(DC)(DC)(DT)(DG)(DA)(DC)(DG) (DA)(DG)(DC)(DA)(DT)(DC)(DA)(DT)(DC)(DA) (DG) (DG)(DG)

+
Macromolecule #29: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 29 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.969294 KDa
SequenceString:
(DC)(DT)(DG)(DT)(DG)(DT)(DG)(DT)(DG)(DT) (DA)(DA)(DA)(DG)(DG)(DC)(DC)(DC)(DG)(DC) (DT)(DT)(DT)(DC)(DC)(DA)(DG)(DT)(DA) (DG)(DG)(DC)(DG)(DG)(DT)(DA)(DT)(DC)(DA) (DG) (DC)(DT)

+
Macromolecule #30: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 30 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.515767 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DG)(DG)(DC)(DG)(DT) (DA)(DA)(DT)(DC)(DA)(DT)(DG)(DT)(DG)(DA) (DG)(DT)(DG)(DA)(DG)(DC)(DT)(DA)(DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #31: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 31 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.489026 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DT)(DG) (DA)(DT)(DC)(DC)(DG)(DG)(DC)(DT)(DC)(DA) (DA)(DA)(DA)(DA)(DG)(DG)(DA)(DT)(DC) (DT)(DT)(DC)(DA)(DC)(DC)(DT)(DT)(DT)(DT) (DT) (DT)

+
Macromolecule #32: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 32 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.03038 KDa
SequenceString:
(DT)(DG)(DG)(DT)(DA)(DG)(DC)(DA)(DA)(DG) (DA)(DT)(DT)(DT)(DT)(DG)(DG)(DT)(DC)(DA) (DT)(DT)(DT)(DT)(DA)(DA)(DA)(DT)(DA) (DT)(DC)(DT)(DG)(DT)(DC)(DA)(DC)(DG)(DG) (DG) (DA)(DG)

+
Macromolecule #33: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 33 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.592535 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DT)(DG) (DT)(DA)(DA)(DA)(DA)(DC)(DG)(DA)(DC)(DG) (DG)(DG)(DT)(DT)(DT)(DT)(DC)(DC)

+
Macromolecule #34: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 34 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.400704 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DA)(DG)(DA)(DT)(DC) (DC)(DT)(DT)(DT)(DT)(DA)(DA)(DA)(DT)(DA) (DG)(DT)(DT)(DG)(DC)(DC)(DT)(DG)(DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #35: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 35 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.146785 KDa
SequenceString:
(DA)(DT)(DG)(DA)(DA)(DG)(DT)(DG)(DA)(DG) (DA)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DA)(DA) (DA)(DC)(DC)(DA)(DC)(DC)(DG)(DC)(DT) (DT)(DA)(DC)(DC)(DT)(DT)(DA)(DA)(DG)(DT) (DG) (DG)(DT)(DG)(DT)(DA)(DA)(DC)(DA) (DG)

+
Macromolecule #36: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 36 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.835253 KDa
SequenceString:
(DT)(DG)(DT)(DG)(DC)(DT)(DG)(DC)(DC)(DT) (DA)(DA)(DA)(DG)(DG)(DT)(DC)(DT)(DA)(DT) (DC)(DC)(DT)(DC)(DC)(DA)(DA)(DC)(DG) (DT)(DC)(DA)(DA)(DA)(DG)(DC)(DT)(DC)(DC) (DG) (DA)(DA)

+
Macromolecule #37: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 37 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 18.109545 KDa
SequenceString: (DT)(DA)(DT)(DT)(DT)(DC)(DG)(DG)(DT)(DT) (DC)(DT)(DT)(DG)(DC)(DG)(DG)(DA)(DA)(DA) (DA)(DG)(DT)(DG)(DG)(DC)(DG)(DC)(DC) (DT)(DG)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DC) (DC) (DG)(DG)(DA)(DT)(DA)(DC) ...String:
(DT)(DA)(DT)(DT)(DT)(DC)(DG)(DG)(DT)(DT) (DC)(DT)(DT)(DG)(DC)(DG)(DG)(DA)(DA)(DA) (DA)(DG)(DT)(DG)(DG)(DC)(DG)(DC)(DC) (DT)(DG)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DC) (DC) (DG)(DG)(DA)(DT)(DA)(DC)(DC)(DT) (DG)(DT)(DC)(DC)(DG)(DC)(DT)(DT)(DT)(DT) (DT)

+
Macromolecule #38: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 38 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.951357 KDa
SequenceString:
(DA)(DG)(DT)(DG)(DA)(DG)(DG)(DC)(DC)(DC) (DC)(DA)(DG)(DT)(DG)(DC)(DT)(DG)(DC)(DA) (DA)(DT)(DG)(DA)(DT)(DA)(DA)(DC)(DA) (DC)(DC)(DA)(DA)(DT)(DG)(DA)(DG)(DA)(DC) (DA) (DA)(DC)

+
Macromolecule #39: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 39 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.980338 KDa
SequenceString:
(DG)(DA)(DA)(DA)(DG)(DT)(DT)(DC)(DC)(DG) (DC)(DC)(DG)(DC)(DG)(DC)(DT)(DT)(DA)(DA) (DT)(DT)(DG)(DC)(DG)(DC)(DG)(DT)(DG) (DG)(DG)(DG)(DA)(DA)(DA)(DT)(DT)(DA)(DA) (DC) (DC)(DA)

+
Macromolecule #40: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 40 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.986334 KDa
SequenceString:
(DT)(DT)(DC)(DG)(DC)(DT)(DA)(DT)(DA)(DC) (DA)(DG)(DG)(DG)(DC)(DG)(DC)(DG)(DT)(DC) (DC)(DT)(DG)(DG)(DC)(DA)(DA)(DG)(DC) (DG)(DA)(DG)(DA)(DA)(DA)(DT)(DT)(DT)(DG) (DT) (DT)(DA)

+
Macromolecule #41: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 41 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.636563 KDa
SequenceString:
(DA)(DG)(DG)(DG)(DC)(DG)(DA)(DC)(DA)(DT) (DT)(DC)(DA)(DG)(DG)(DC)(DT)(DG)(DC)(DG) (DC)(DA)(DA)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #42: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 42 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.598531 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DT) (DG)(DG)(DG)(DA)(DC)(DA)(DG)(DT)(DC)(DA) (DC)(DG)(DA)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #43: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 43 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.565052 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DC)(DC)(DC) (DC)(DG)(DT)(DC)(DG)(DT)(DG)(DT)(DA)(DG) (DA)(DT)(DA)(DA)(DG)(DC)(DC)(DA)(DG) (DC)(DC)(DG)(DG)(DA)(DA)(DG)(DT)(DT)(DT) (DT) (DT)

+
Macromolecule #44: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 44 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.904186 KDa
SequenceString: (DG)(DG)(DC)(DT)(DT)(DA)(DC)(DA)(DG)(DA) (DT)(DT)(DT)(DA)(DC)(DT)(DT)(DT)(DA)(DT) (DC)(DT)(DA)(DC)(DT)(DG)(DT)(DC)(DT) (DC)(DG)(DT)(DT)(DG)(DT)(DA)(DA)(DA)(DC) (DG) (DT)(DT)(DC)(DT)(DT)(DC) ...String:
(DG)(DG)(DC)(DT)(DT)(DA)(DC)(DA)(DG)(DA) (DT)(DT)(DT)(DA)(DC)(DT)(DT)(DT)(DA)(DT) (DC)(DT)(DA)(DC)(DT)(DG)(DT)(DC)(DT) (DC)(DG)(DT)(DT)(DG)(DT)(DA)(DA)(DA)(DC) (DG) (DT)(DT)(DC)(DT)(DT)(DC)(DG)(DT) (DT)(DT)(DT)(DT)

+
Macromolecule #45: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 45 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 14.889495 KDa
SequenceString:
(DC)(DG)(DC)(DT)(DC)(DA)(DC)(DC)(DG)(DG) (DC)(DT)(DC)(DC)(DC)(DA)(DT)(DC)(DT)(DG) (DG)(DC)(DA)(DC)(DC)(DT)(DA)(DT)(DC) (DT)(DA)(DC)(DG)(DG)(DC)(DT)(DC)(DT)(DG) (DC) (DT)(DG)(DC)(DG)(DC)(DT)(DG)(DT) (DA)

+
Macromolecule #46: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 46 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 16.091256 KDa
SequenceString: (DT)(DT)(DT)(DT)(DT)(DG)(DG)(DA)(DG)(DC) (DG)(DG)(DG)(DC)(DG)(DC)(DT)(DA)(DG)(DG) (DG)(DC)(DG)(DC)(DC)(DA)(DT)(DT)(DC) (DG)(DC)(DT)(DC)(DG)(DG)(DT)(DG)(DC)(DT) (DG) (DC)(DC)(DT)(DA)(DA)(DG) ...String:
(DT)(DT)(DT)(DT)(DT)(DG)(DG)(DA)(DG)(DC) (DG)(DG)(DG)(DC)(DG)(DC)(DT)(DA)(DG)(DG) (DG)(DC)(DG)(DC)(DC)(DA)(DT)(DT)(DC) (DG)(DC)(DT)(DC)(DG)(DG)(DT)(DG)(DC)(DT) (DG) (DC)(DC)(DT)(DA)(DA)(DG)(DT)(DC) (DA)(DT)(DA)(DG)

+
Macromolecule #47: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 47 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 16.013289 KDa
SequenceString: (DT)(DT)(DT)(DT)(DT)(DG)(DG)(DC)(DC)(DG) (DA)(DG)(DC)(DG)(DC)(DA)(DG)(DA)(DA)(DG) (DT)(DG)(DG)(DT)(DC)(DC)(DT)(DG)(DC) (DA)(DA)(DT)(DC)(DA)(DG)(DC)(DA)(DA)(DT) (DA) (DA)(DA)(DC)(DC)(DA)(DC) ...String:
(DT)(DT)(DT)(DT)(DT)(DG)(DG)(DC)(DC)(DG) (DA)(DG)(DC)(DG)(DC)(DA)(DG)(DA)(DA)(DG) (DT)(DG)(DG)(DT)(DC)(DC)(DT)(DG)(DC) (DA)(DA)(DT)(DC)(DA)(DG)(DC)(DA)(DA)(DT) (DA) (DA)(DA)(DC)(DC)(DA)(DC)(DT)(DA) (DC)(DG)(DA)(DT)

+
Macromolecule #48: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 48 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.799233 KDa
SequenceString:
(DG)(DC)(DT)(DT)(DG)(DA)(DC)(DA)(DA)(DC) (DC)(DA)(DC)(DC)(DA)(DC)(DC)(DC)(DG)(DC) (DG)(DT)(DG)(DA)(DC)(DC)(DC)(DA)(DC) (DT)(DA)(DT)(DT)(DA)(DA)(DG)(DG)(DC)(DA) (DG) (DC)(DA)

+
Macromolecule #49: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 49 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.106392 KDa
SequenceString:
(DG)(DC)(DC)(DG)(DG)(DC)(DG)(DT)(DC)(DG) (DG)(DA)(DA)(DC)(DC)(DA)(DA)(DG)(DG)(DC) (DG)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DC) (DG)(DG)(DC)(DC)(DA)(DG)(DT)(DG)(DA)(DG) (DC) (DG)(DA)

+
Macromolecule #50: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 50 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.942291 KDa
SequenceString:
(DA)(DA)(DC)(DG)(DT)(DG)(DG)(DT)(DG)(DT) (DA)(DG)(DC)(DG)(DG)(DT)(DC)(DA)(DC)(DG) (DC)(DG)(DC)(DG)(DC)(DC)(DG)(DC)(DT) (DT)(DA)(DC)(DG)(DC)(DC)(DC)(DA)(DT)(DA) (DA) (DA)(DG)

+
Macromolecule #51: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 51 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.679128 KDa
SequenceString:
(DA)(DG)(DT)(DA)(DA)(DG)(DT)(DA)(DA)(DG) (DT)(DA)(DT)(DA)(DG)(DG)(DA)(DT)(DC)(DT) (DC)(DG)(DG)(DT)(DG)(DG)(DT)(DT)(DT) (DC)(DC)(DC)(DT)(DC)(DG)(DT)(DG)(DC)(DG) (DC) (DT)

+
Macromolecule #52: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 52 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.792939 KDa
SequenceString:
(DT)(DG)(DC)(DC)(DG)(DG)(DG)(DT)(DG)(DC) (DT)(DA)(DC)(DA)(DG)(DG)(DC)(DA)(DT)(DC) (DG)(DA)(DA)(DA)(DA)(DA)(DC)(DC)(DG) (DG)(DA)(DG)(DC)(DC)(DC)

+
Macromolecule #53: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 53 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.664115 KDa
SequenceString:
(DC)(DC)(DG)(DA)(DT)(DG)(DT)(DG)(DT)(DC) (DT)(DG)(DA)(DC)(DA)(DA)(DG)(DA)(DT)(DT) (DA)(DC)(DG)(DC)(DG)(DC)(DA)(DG)(DT) (DT)(DT)(DC)(DT)(DA)(DC)(DG)(DG)(DG)(DG) (DT) (DT)

+
Macromolecule #54: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 54 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.916889 KDa
SequenceString:
(DG)(DA)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DC) (DC)(DA)(DG)(DG)(DC)(DC)(DA)(DG)(DT)(DG) (DA)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG) (DA)(DA)(DT)(DT)(DC)(DG)(DC)(DG)(DC)(DT) (DT) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #55: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 55 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.514778 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DG)(DG)(DG)(DG)(DT)(DC)(DA)(DA)(DG) (DA)(DA)(DA)(DG)(DC)(DG)(DA)(DA)(DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #56: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 56 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.609098 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DA)(DA)(DT)(DA)(DG) (DT)(DT)(DT)(DG)(DC)(DG)(DC)(DT)(DG)(DA) (DA)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DT) (DA)(DG)(DC)(DT)(DC)(DC)(DT)(DT)(DT)(DT) (DT) (DT)

+
Macromolecule #57: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 57 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.835308 KDa
SequenceString:
(DC)(DT)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DC) (DA)(DC)(DT)(DA)(DT)(DT)(DA)(DA)(DA)(DG) (DA)(DT)(DT)(DA)(DT)(DA)(DA)(DA)(DA) (DA)(DT)(DC)(DA)(DG)(DC)(DC)(DC)(DC)(DC) (DG) (DA)(DA)

+
Macromolecule #58: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 58 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 9.448138 KDa
SequenceString:
(DA)(DC)(DA)(DA)(DG)(DA)(DG)(DA)(DA)(DC) (DC)(DA)(DT)(DC)(DA)(DC)(DC)(DC)(DT)(DA) (DA)(DT)(DC)(DA)(DA)(DG)(DT)(DT)(DT) (DT)(DT)

+
Macromolecule #59: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 59 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.647569 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DG)(DT)(DT)(DG)(DT) (DT)(DC)(DC)(DA)(DG)(DT)(DT)(DT)(DG)(DG) (DA)(DC)(DG)(DA)(DG)(DA)(DT)(DA)

+
Macromolecule #60: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 60 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.323618 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DG)(DT) (DC)(DC)(DT)(DC)(DC)(DG)(DA)(DA)(DT)(DG) (DC)(DC)(DA)(DT)(DC)(DC)(DG)(DT)(DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #61: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 61 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.496788 KDa
SequenceString:
(DC)(DA)(DG)(DA)(DA)(DG)(DT)(DT)(DT)(DT) (DG)(DG)(DT)(DA)(DA)(DA)(DC)(DG)(DT)(DT) (DG)(DA)(DA)(DA)(DC)(DT)(DC)(DA)(DC) (DG)(DT)(DT)(DA)(DT)

+
Macromolecule #62: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 62 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.043399 KDa
SequenceString:
(DC)(DC)(DG)(DC)(DA)(DG)(DT)(DT)(DT)(DA) (DA)(DA)(DA)(DG)(DG)(DC)(DT)(DG)(DT)(DT) (DG)(DA)(DG)(DA)(DT)(DC)(DC)(DA)(DG) (DT)(DT)(DC)(DG)(DA)(DT)(DA)(DA)(DG)(DG) (DG) (DA)(DA)

+
Macromolecule #63: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 63 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.664894 KDa
SequenceString:
(DA)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DG)(DC) (DA)(DA)(DA)(DA)(DG)(DT)(DA)(DA)(DC)(DC) (DC)(DA)(DC)(DT)(DC)(DG)(DT)(DG)(DC) (DA)(DC)(DC)(DC)(DA)(DA)

+
Macromolecule #64: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 64 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.718868 KDa
SequenceString:
(DT)(DC)(DA)(DA)(DA)(DA)(DG)(DA)(DA)(DA) (DA)(DA)(DC)(DA)(DC)(DT)(DG)(DA)(DT)(DC) (DT)(DT)(DC)(DA)(DG)(DC)(DA)(DT)(DC) (DT)(DT)(DT)(DT)(DA)(DC)(DT)(DT)(DT)(DC) (DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #65: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 65 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.907027 KDa
SequenceString:
(DA)(DT)(DA)(DG)(DG)(DC)(DA)(DA)(DT)(DA) (DG)(DG)(DG)(DG)(DT)(DT)(DC)(DC)(DG)(DC) (DG)(DA)(DT)(DT)(DT)(DA)(DG)(DA)(DT) (DG)(DT)(DT)(DG)(DA)(DA)

+
Macromolecule #66: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 66 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.606558 KDa
SequenceString:
(DA)(DT)(DT)(DC)(DG)(DC)(DG)(DT)(DT)(DG) (DT)(DA)(DA)(DG)(DC)(DG)(DT)(DT)(DA)(DA) (DT)(DA)(DT)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #67: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 67 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.658576 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DG)(DT) (DT)(DA)(DA)(DA)(DG)(DG)(DG)(DT)(DT)(DG) (DA)(DG)(DT)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #68: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 68 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.518994 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DA)(DG)(DA)(DT)(DG) (DC)(DT)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DG) (DA)(DC)(DT)(DG)(DC)(DT)(DC)(DT)(DT) (DG)(DC)(DC)(DC)(DG)(DG)(DC)(DT)(DT)(DT) (DT) (DT)

+
Macromolecule #69: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 69 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.735943 KDa
SequenceString:
(DC)(DT)(DC)(DA)(DA)(DC)(DC)(DA)(DA)(DG) (DT)(DC)(DA)(DT)(DT)(DC)(DT)(DG)(DA)(DA) (DA)(DT)(DG)(DG)(DT)(DT)(DA)(DT)(DT) (DT)(DT)(DT)(DA)(DG)(DA)

+
Macromolecule #70: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 70 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.993372 KDa
SequenceString:
(DA)(DA)(DG)(DT)(DG)(DC)(DC)(DA)(DG)(DC) (DG)(DG)(DA)(DT)(DC)(DT)(DT)(DT)(DT)(DT) (DC)(DA)(DA)(DT)(DA)(DT)(DT)(DA)(DG) (DG)(DT)(DG)(DA)(DG)(DC)(DA)(DA)(DT)(DA) (DG) (DA)(DC)

+
Macromolecule #71: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 71 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.095507 KDa
SequenceString:
(DA)(DA)(DA)(DA)(DT)(DA)(DA)(DA)(DC)(DG) (DA)(DA)(DT)(DA)(DG)(DT)(DG)(DT)(DA)(DT) (DG)(DA)(DT)(DA)(DG)(DC)(DA)(DG)(DA) (DA)(DG)(DC)(DG)(DA)(DC)(DA)(DC)(DG)(DG) (DA) (DA)(DA)

+
Macromolecule #72: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 72 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 13.926958 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DG)(DG) (DG)(DT)(DT)(DA)(DT)(DT)(DG)(DT)(DC)(DT) (DC)(DA)(DT)(DG)(DA)(DC)(DC)(DT)(DA) (DA)(DA)(DT)(DT)(DA)(DA)(DA)(DT)(DT)(DG) (DG) (DA)(DA)(DG)(DG)(DG)

+
Macromolecule #73: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 73 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 9.541168 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DG)(DT)(DC)(DA)(DA) (DT)(DA)(DC)(DG)(DG)(DG)(DA)(DT)(DA)(DA) (DT)(DA)(DC)(DC)(DC)(DA)(DT)(DT)(DG) (DG)(DA)

+
Macromolecule #74: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 74 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.860217 KDa
SequenceString:
(DG)(DC)(DG)(DC)(DC)(DA)(DC)(DC)(DG)(DG) (DC)(DG)(DA)(DC)(DC)(DG)(DA)(DG)(DT)(DT) (DG)(DG)(DT)(DG)(DA)(DG)(DT)(DA)(DA) (DT)(DT)(DC)(DT)(DC)(DT)(DC)(DG)(DC)(DC) (DT) (DC)(DC)

+
Macromolecule #75: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 75 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 15.077726 KDa
SequenceString:
(DT)(DA)(DC)(DT)(DC)(DA)(DT)(DA)(DA)(DA) (DA)(DT)(DG)(DC)(DA)(DA)(DA)(DT)(DC)(DC) (DC)(DA)(DC)(DG)(DT)(DG)(DG)(DA)(DA) (DG)(DG)(DG)(DC)(DG)(DA)(DT)(DG)(DG)(DC) (DC) (DC)(DA)(DC)(DA)(DC)(DT)(DA)(DA) (DA)

+
Macromolecule #76: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 76 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.664116 KDa
SequenceString:
(DT)(DA)(DA)(DG)(DG)(DC)(DT)(DG)(DT)(DT) (DA)(DT)(DC)(DA)(DG)(DG)(DC)(DG)(DT)(DG) (DG)(DT)(DG)(DT)(DC)(DA)(DC)(DG)(DG) (DT)(DT)(DC)(DC)(DC)(DA)(DA)(DC)(DG)(DA) (DT) (DT)

+
Macromolecule #77: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 77 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 12.860284 KDa
SequenceString:
(DG)(DG)(DA)(DA)(DG)(DG)(DC)(DA)(DC)(DT) (DC)(DT)(DT)(DC)(DA)(DC)(DA)(DT)(DA)(DT) (DT)(DT)(DG)(DA)(DA)(DT)(DG)(DT)(DC) (DA)(DC)(DA)(DT)(DT)(DT)(DT)(DC)(DA)(DT) (DT) (DT)(DT)

+
Macromolecule #78: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 78 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 10.392678 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DC)(DC)(DA)(DG)(DC) (DG)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DT)(DG) (DA)(DA)(DG)(DC)(DA)(DT)(DT)(DT)(DA) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #79: STAPLE STRAND

MacromoleculeName: STAPLE STRAND / type: dna / ID: 79 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 11.387317 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DG)(DG)(DG)(DC)(DG) (DA)(DA)(DA)(DA)(DC)(DT)(DC)(DT)(DC)(DA) (DA)(DG)(DG)(DA)(DC)(DC)(DC)(DC)(DA) (DT)(DG)(DT)(DT)(DG)(DT)(DG)(DT)

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 10.38 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 42209
FSC plot (resolution estimation)

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more