+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-10625 | |||||||||
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Title | Cin8 motor domain (short loop 8) bound to microtubules | |||||||||
Map data | Cin8 motor domain (short loop 8) bound to microtubules | |||||||||
Sample |
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Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 6.1 Å | |||||||||
Authors | Siegler N / Singh SK / Zalk R / Debs GE / Sindelar CV / Gheber L / Zarivach R | |||||||||
Funding support | Israel, 1 items
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Citation | Journal: To Be Published Title: Cin8 motor domain (short loop 8) bound to microtubules Authors: Siegler N / Singh SK / Zalk R / Debs GE / Sindelar CV / Gheber L | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_10625.map.gz | 51.4 MB | EMDB map data format | |
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Header (meta data) | emd-10625-v30.xml emd-10625.xml | 10.3 KB 10.3 KB | Display Display | EMDB header |
Images | emd_10625.png | 132.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-10625 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10625 | HTTPS FTP |
-Validation report
Summary document | emd_10625_validation.pdf.gz | 268 KB | Display | EMDB validaton report |
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Full document | emd_10625_full_validation.pdf.gz | 267.1 KB | Display | |
Data in XML | emd_10625_validation.xml.gz | 6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10625 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10625 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_10625.map.gz / Format: CCP4 / Size: 58.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cin8 motor domain (short loop 8) bound to microtubules | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN
Entire | Name: ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN |
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Components |
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-Supramolecule #1: ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN
Supramolecule | Name: ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all / Details: ADDED 0.1 mM AMP-PNP, 10 micromolar Taxol |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
-Macromolecule #1: Cin8, microtubules
Macromolecule | Name: Cin8, microtubules / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE ISMKSSVVVN VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS QDLIFDEVAG PLFQDFIKGY NCTVLVYGMT STGKTYTMTG DEKLYNGELS DAAGIIPRVL LKLFDTLELQ ...String: MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE ISMKSSVVVN VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS QDLIFDEVAG PLFQDFIKGY NCTVLVYGMT STGKTYTMTG DEKLYNGELS DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF IELYNEELKD LLDSNSNGSS NTGFDGQFMK KLRIFDNNNN NSSIYIQNLQ EFHITNAMEG LNLLQKGLKH RQVASTKMND FSSRSHTIFT ITLYKKHQDE LFRISKMNLV DLAGSENINR SGALNQRAKE AGSINQSLLT LGRVINALVD KSGHIPFRES KLTRLLQDSL GGNTKTALIA TISPAKVTSE ETCSTLEYAS KAKNIKNKPQ LGSFIMKDIL LEHHHHHH |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 6.9 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE / Instrument: HOMEMADE PLUNGER |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 80.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.3 mm |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 82.18 Å Applied symmetry - Helical parameters - Δ&Phi: -0.53 ° Applied symmetry - Helical parameters - Axial symmetry: C14 (14 fold cyclic) Resolution.type: BY AUTHOR / Resolution: 6.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 47551 |
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CTF correction | Software - Name: Gctf (ver. 1.06) |
Segment selection | Number selected: 47551 / Software - Name: RELION (ver. 2.1) / Details: Number of particles seleced for reconstruction |
Final angle assignment | Type: NOT APPLICABLE / Software - Name: RELION (ver. 2.1) |