|
|
eF-site ID
|
5fw2-ACD |
|
PDB Code
|
5fw2 |
|
Chain
|
A, C, D |
|
click to enlarge
|
|
|
Title
|
Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA |
|
Classification
|
HYDROLASE/DNA/RNA |
|
Compound
|
SGRNA |
|
Source
|
(5FW2) |
|
|
Sequence
|
A: |
AUAACUCAAUUUGUAAAAAAGUUUUAGAGCUAGAAAUAGC
AAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGU
G
|
| C: |
CAATCTCATTTTTTACAAATTGAGTTAT
|
| D: |
AAATGAGATTG
|
|
|
Description
|
|
Functional site
|
|
|
1)
|
chain |
A |
| residue |
21 |
| type |
|
| sequence |
G
|
| description |
BINDING SITE FOR RESIDUE K A 1182
|
| source |
: AC1
|
|
|
2)
|
chain |
A |
| residue |
12 |
| type |
|
| sequence |
U
|
| description |
BINDING SITE FOR RESIDUE K A 1183
|
| source |
: AC2
|
|
|
3)
|
chain |
A |
| residue |
13 |
| type |
|
| sequence |
G
|
| description |
BINDING SITE FOR RESIDUE K A 1183
|
| source |
: AC2
|
|
|
4)
|
chain |
A |
| residue |
50 |
| type |
|
| sequence |
U
|
| description |
BINDING SITE FOR RESIDUE MG A 1084
|
| source |
: AC3
|
|
|
5)
|
chain |
A |
| residue |
68 |
| type |
|
| sequence |
A
|
| description |
BINDING SITE FOR RESIDUE K B 2366
|
| source |
: AC4
|
|
|
6)
|
chain |
A |
| residue |
65 |
| type |
|
| sequence |
A
|
| description |
BINDING SITE FOR RESIDUE K B 2368
|
| source |
: AC6
|
|
|
7)
|
chain |
A |
| residue |
66 |
| type |
|
| sequence |
U
|
| description |
BINDING SITE FOR RESIDUE K B 2368
|
| source |
: AC6
|
|
|
|