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5FW2

Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA

Summary for 5FW2
Entry DOI10.2210/pdb5fw2/pdb
Related5FW1 5FW3
DescriptorSGRNA, CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, TARGET DNA STRAND, ... (7 entities in total)
Functional Keywordshydrolase-dna-rna complex, crispr, cas9, endonuclease, pam, genome editing, rnp, protein-rna complex, hydrolase/dna/rna
Biological sourceSTREPTOCOCCUS PYOGENES
More
Total number of polymer chains4
Total formula weight198422.66
Authors
Anders, C.,Bargsten, K.,Jinek, M. (deposition date: 2016-02-11, release date: 2016-06-15, Last modification date: 2024-01-10)
Primary citationAnders, C.,Bargsten, K.,Jinek, M.
Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
Mol.Cell, 61:895-, 2016
Cited by
PubMed: 26990992
DOI: 10.1016/J.MOLCEL.2016.02.020
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.676 Å)
Structure validation

217705

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