eF-site ID 4ej1-ABCD PDB Code 4ej1 Chain A, B, C, D click to enlarge Title Binding of Nb113 camelid antibody fragment with the binary DHFR:folate complex Classification Oxidoreductase/Immune system Compound Dihydrofolate reductase Source Escherichia coli (strain K12) (4EJ1) Sequence A:  MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPV IMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDE AIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVE GDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR B:  MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPV IMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDE AIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVE GDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR C:  QLQESGGGLVQAGGSLRLSCTASGRTFSSYAMGWFRQTPG KEREFVAAITWGGSTTLYADSVKGRFTMSRDNAKNTVYLQ MNSLKPEDTAVYYCAADGSQYRSTYSFRDKPDYGSWGQGT QVTVSS D:  QLQESGGGLVQAGGSLRLSCTASGRTFSSYAMGWFRQTPG KEREFVAAITWGGSTTLYADSVKGRFTMSRDNAKNTVYLQ MNSLKPEDTAVYYCAADGSQYRSTYSFRDKPDYGSWGQGT QVTVSS Description (1)  Dihydrofolate reductase (E.C.1.5.1.3), Nb113 camelid antibody fragment Functional site 1) chain A residue 5 type sequence I description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 2) chain A residue 6 type sequence A description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 3) chain A residue 7 type sequence A description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 4) chain A residue 17 type sequence E description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 5) chain A residue 27 type sequence D description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 6) chain A residue 28 type sequence L description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 7) chain A residue 31 type sequence F description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 8) chain A residue 32 type sequence K description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 9) chain A residue 50 type sequence I description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 10) chain A residue 52 type sequence R description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 11) chain A residue 57 type sequence R description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 12) chain A residue 94 type sequence I description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 13) chain A residue 100 type sequence Y description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 14) chain A residue 113 type sequence T description BINDING SITE FOR RESIDUE FOL A 201 source : AC1 15) chain A residue 43 type sequence G description BINDING SITE FOR RESIDUE PO4 A 202 source : AC2 16) chain A residue 44 type sequence R description BINDING SITE FOR RESIDUE PO4 A 202 source : AC2 17) chain A residue 45 type sequence H description BINDING SITE FOR RESIDUE PO4 A 202 source : AC2 18) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE PO4 A 202 source : AC2 19) chain A residue 96 type sequence G description BINDING SITE FOR RESIDUE PO4 A 202 source : AC2 20) chain A residue 44 type sequence R description BINDING SITE FOR RESIDUE PO4 A 203 source : AC3 21) chain A residue 63 type sequence S description BINDING SITE FOR RESIDUE PO4 A 203 source : AC3 22) chain A residue 64 type sequence S description BINDING SITE FOR RESIDUE PO4 A 203 source : AC3 23) chain A residue 144 type sequence D description BINDING SITE FOR RESIDUE PO4 A 204 source : AC4 24) chain A residue 145 type sequence A description BINDING SITE FOR RESIDUE PO4 A 204 source : AC4 25) chain A residue 146 type sequence Q description BINDING SITE FOR RESIDUE PO4 A 204 source : AC4 26) chain C residue 76 type sequence K description BINDING SITE FOR RESIDUE PO4 A 204 source : AC4 27) chain B residue 5 type sequence I description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 28) chain B residue 6 type sequence A description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 29) chain B residue 7 type sequence A description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 30) chain B residue 17 type sequence E description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 31) chain B residue 27 type sequence D description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 32) chain B residue 31 type sequence F description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 33) chain B residue 32 type sequence K description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 34) chain B residue 54 type sequence L description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 35) chain B residue 57 type sequence R description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 36) chain B residue 94 type sequence I description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 37) chain B residue 100 type sequence Y description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 38) chain B residue 113 type sequence T description BINDING SITE FOR RESIDUE FOL B 201 source : AC5 39) chain B residue 43 type sequence G description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 40) chain B residue 44 type sequence R description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 41) chain B residue 45 type sequence H description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 42) chain B residue 46 type sequence T description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 43) chain B residue 96 type sequence G description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 44) chain B residue 99 type sequence V description BINDING SITE FOR RESIDUE PO4 B 202 source : AC6 45) chain B residue 44 type sequence R description BINDING SITE FOR RESIDUE PO4 B 203 source : AC7 46) chain B residue 63 type sequence S description BINDING SITE FOR RESIDUE PO4 B 203 source : AC7 47) chain B residue 64 type sequence S description BINDING SITE FOR RESIDUE PO4 B 203 source : AC7 48) chain B residue 98 type sequence R description BINDING SITE FOR RESIDUE PO4 B 203 source : AC7 49) chain D residue 27 type sequence R description BINDING SITE FOR RESIDUE PO4 B 203 source : AC7 50) chain B residue 144 type sequence D description BINDING SITE FOR RESIDUE PO4 B 204 source : AC8 51) chain B residue 145 type sequence A description BINDING SITE FOR RESIDUE PO4 B 204 source : AC8 52) chain B residue 146 type sequence Q description BINDING SITE FOR RESIDUE PO4 B 204 source : AC8 53) chain D residue 76 type sequence K description BINDING SITE FOR RESIDUE PO4 B 204 source : AC8 54) chain A residue 13-35 type prosite sequence VIGMENAMPWNLPADLAWFKRNT description DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT source prosite : PS00075 55) chain A residue 5 type BINDING sequence I description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 56) chain B residue 113 type BINDING sequence T description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 57) chain A residue 27 type BINDING sequence D description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 58) chain A residue 52 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 59) chain A residue 57 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 60) chain A residue 113 type BINDING sequence T description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 61) chain B residue 5 type BINDING sequence I description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 62) chain B residue 27 type BINDING sequence D description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 63) chain B residue 52 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 64) chain B residue 57 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 65) chain A residue 7 type BINDING sequence A description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 66) chain B residue 63 type BINDING sequence S description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 67) chain B residue 76 type BINDING sequence K description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 68) chain B residue 95 type BINDING sequence G description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 69) chain A residue 13 type BINDING sequence V description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 70) chain A residue 45 type BINDING sequence H description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 71) chain A residue 63 type BINDING sequence S description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 72) chain A residue 76 type BINDING sequence K description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 73) chain A residue 95 type BINDING sequence G description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 74) chain B residue 7 type BINDING sequence A description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 75) chain B residue 13 type BINDING sequence V description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 76) chain B residue 45 type BINDING sequence H description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2

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