eF-site ID 1u72-A PDB Code 1u72 Chain A click to enlarge Title Understanding the Role of Leu22 Variants in Methotrexate Resistance: Comparison of Wild-type and Leu22Arg Variant Mouse and Human Dihydrfolate Reductase Ternary Crystal Complexes with Methotrexate and NADPH Classification OXIDOREDUCTASE Compound Dihydrofolate reductase Source Homo sapiens (Human) (DYR_HUMAN) Sequence A:  VGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTT SSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELK EPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSV YKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLP EYPGVLSDVQEEKGIKYKFEVYEKND Description Functional site 1) chain A residue 8 type sequence V description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 2) chain A residue 9 type sequence A description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 3) chain A residue 16 type sequence I description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 4) chain A residue 17 type sequence G description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 5) chain A residue 20 type sequence G description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 6) chain A residue 21 type sequence D description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 7) chain A residue 22 type sequence L description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 8) chain A residue 24 type sequence W description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 9) chain A residue 53 type sequence G description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 10) chain A residue 54 type sequence K description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 11) chain A residue 55 type sequence K description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 12) chain A residue 56 type sequence T description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 13) chain A residue 59 type sequence S description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 14) chain A residue 75 type sequence L description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 15) chain A residue 76 type sequence S description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 16) chain A residue 77 type sequence R description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 17) chain A residue 78 type sequence E description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 18) chain A residue 91 type sequence R description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 19) chain A residue 115 type sequence V description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 20) chain A residue 117 type sequence G description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 21) chain A residue 118 type sequence S description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 22) chain A residue 119 type sequence S description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 23) chain A residue 120 type sequence V description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 24) chain A residue 121 type sequence Y description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 25) chain A residue 146 type sequence T description BINDING SITE FOR RESIDUE NDP A 187 source : AC1 26) chain A residue 7 type sequence I description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 27) chain A residue 8 type sequence V description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 28) chain A residue 9 type sequence A description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 29) chain A residue 22 type sequence L description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 30) chain A residue 30 type sequence E description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 31) chain A residue 31 type sequence F description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 32) chain A residue 34 type sequence F description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 33) chain A residue 35 type sequence Q description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 34) chain A residue 59 type sequence S description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 35) chain A residue 60 type sequence I description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 36) chain A residue 61 type sequence P description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 37) chain A residue 64 type sequence N description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 38) chain A residue 67 type sequence L description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 39) chain A residue 70 type sequence R description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 40) chain A residue 115 type sequence V description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 41) chain A residue 121 type sequence Y description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 42) chain A residue 136 type sequence T description BINDING SITE FOR RESIDUE MTX A 188 source : AC2 43) chain A residue 23 type catalytic sequence P description 490 source MCSA : MCSA1 44) chain A residue 31 type catalytic sequence F description 490 source MCSA : MCSA1 45) chain A residue 10 type BINDING sequence V description BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 source Swiss-Prot : SWS_FT_FI1 46) chain A residue 16 type BINDING sequence I description BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 source Swiss-Prot : SWS_FT_FI1 47) chain A residue 55 type BINDING sequence K description BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 source Swiss-Prot : SWS_FT_FI1 48) chain A residue 77 type BINDING sequence R description BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 source Swiss-Prot : SWS_FT_FI1 49) chain A residue 117 type BINDING sequence G description BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 source Swiss-Prot : SWS_FT_FI1 50) chain A residue 15-38 type prosite sequence GIGKNGDLPWPPLRNEFRYFQRMT description DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfryFqrmT source prosite : PS00075 51) chain A residue 31 type BINDING sequence F description BINDING => ECO:0000305|PubMed:2248959 source Swiss-Prot : SWS_FT_FI2 52) chain A residue 65 type BINDING sequence R description BINDING => ECO:0000305|PubMed:2248959 source Swiss-Prot : SWS_FT_FI2 53) chain A residue 71 type BINDING sequence I description BINDING => ECO:0000305|PubMed:2248959 source Swiss-Prot : SWS_FT_FI2

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