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Yorodumi- EMDB-1269: Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection. -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-1269 | |||||||||
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Title | Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection. | |||||||||
Map data | ccp4 map of the tip of the bacteriophage spp1 | |||||||||
Sample |
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Biological species | Bacillus phage SPP1 (virus) | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 20.0 Å | |||||||||
Authors | Plisson C / White HE / Auzat I / Sao-jose C / Lhuillier S / Tavares P / Orlova EV | |||||||||
Citation | Journal: EMBO J / Year: 2007 Title: Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection. Authors: Celia Plisson / Helen E White / Isabelle Auzat / Amineh Zafarani / Carlos São-José / Sophie Lhuillier / Paulo Tavares / Elena V Orlova / Abstract: The majority of known bacteriophages have long noncontractile tails (Siphoviridae) that serve as a pipeline for genome delivery into the host cytoplasm. The tail extremity distal from the phage head ...The majority of known bacteriophages have long noncontractile tails (Siphoviridae) that serve as a pipeline for genome delivery into the host cytoplasm. The tail extremity distal from the phage head is an adsorption device that recognises the bacterial receptor at the host cell surface. This interaction generates a signal transmitted to the head that leads to DNA release. We have determined structures of the bacteriophage SPP1 tail before and after DNA ejection. The results reveal extensive structural rearrangements in the internal wall of the tail tube. We propose that the adsorption device-receptor interaction triggers a conformational switch that is propagated as a domino-like cascade along the 1600 A-long helical tail structure to reach the head-to-tail connector. This leads to opening of the connector culminating in DNA exit from the head into the host cell through the tail tube. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_1269.map.gz | 124.6 KB | EMDB map data format | |
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Header (meta data) | emd-1269-v30.xml emd-1269.xml | 9.7 KB 9.7 KB | Display Display | EMDB header |
Images | 1269.gif | 18.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-1269 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1269 | HTTPS FTP |
-Validation report
Summary document | emd_1269_validation.pdf.gz | 160.5 KB | Display | EMDB validaton report |
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Full document | emd_1269_full_validation.pdf.gz | 159.6 KB | Display | |
Data in XML | emd_1269_validation.xml.gz | 4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1269 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1269 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_1269.map.gz / Format: CCP4 / Size: 3.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | ccp4 map of the tip of the bacteriophage spp1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Tip of the Tail of the bacteriophage SPP1
Entire | Name: Tip of the Tail of the bacteriophage SPP1 |
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Components |
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-Supramolecule #1000: Tip of the Tail of the bacteriophage SPP1
Supramolecule | Name: Tip of the Tail of the bacteriophage SPP1 / type: sample / ID: 1000 Details: The total number of proteins that compose the tail Tip is still under investigation. Number unique components: 2 |
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-Macromolecule #1: interface tail-tip
Macromolecule | Name: interface tail-tip / type: protein_or_peptide / ID: 1 / Name.synonym: gp19.1 / Recombinant expression: No |
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Source (natural) | Organism: Bacillus phage SPP1 (virus) / synonym: Bacteriophage SPP1 |
Molecular weight | Experimental: 28.5 KDa |
-Macromolecule #2: tip fiber
Macromolecule | Name: tip fiber / type: protein_or_peptide / ID: 2 / Name.synonym: gp21 / Recombinant expression: No |
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Source (natural) | Organism: Bacillus phage SPP1 (virus) / synonym: Bacteriophage SPP1 |
Molecular weight | Experimental: 123 KDa |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 / Details: 100 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2 |
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Staining | Type: NEGATIVE Details: 3.5 microliter of sample was applied onto carbon-coated copper grids, which were glow-discharged for 30 seconds. The sample was left for 1-2 minutes on the grids before blotting and staining ...Details: 3.5 microliter of sample was applied onto carbon-coated copper grids, which were glow-discharged for 30 seconds. The sample was left for 1-2 minutes on the grids before blotting and staining with a solution of 2% uranyl acetate. |
Grid | Details: 300 mesh copper grid |
Vitrification | Cryogen name: NONE |
-Electron microscopy
Microscope | FEI TECNAI 12 |
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Temperature | Average: 298 K |
Alignment procedure | Legacy - Astigmatism: objective lens astigmatism was corrected at 150,000 times magnification |
Details | Low Dose Imaging |
Image recording | Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Digitization - Sampling interval: 7 µm / Number real images: 20 / Average electron dose: 10 e/Å2 / Od range: 2.5 / Bits/pixel: 8 |
Electron beam | Acceleration voltage: 120 kV / Electron source: TUNGSTEN HAIRPIN |
Electron optics | Calibrated magnification: 43475 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 42000 |
Sample stage | Specimen holder: Gatan single tilt negative stain holder / Specimen holder model: OTHER |
-Image processing
Final reconstruction | Applied symmetry - Point group: C3 (3 fold cyclic) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: IMAGIC Details: The 3D reconstruction has been caried out using a C3 symmetry and the exact-filter back projection method. Number images used: 364 |
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