+Open data
-Basic information
Entry | Database: PDB / ID: 2nan | ||||||
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Title | NMR structure of human DCL-1 (CD302) extracellular domain | ||||||
Components | CD302 antigen | ||||||
Keywords | IMMUNE SYSTEM / C-type lectin like receptor | ||||||
Function / homology | Function and homology information microvillus / phagocytosis / filopodium / signaling receptor activity / cell cortex / carbohydrate binding / membrane => GO:0016020 / external side of plasma membrane / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics, torsion angle dynamics | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Pospisilova, E. / Kukacka, Z. / Kavan, D. / Novak, P. / Chmelik, J. | ||||||
Citation | Journal: To be Published Title: NMR structure of human DCL-1 (CD302) extracellular domain Authors: Pospisilova, E. / Kukacka, Z. / Kavan, D. / Novak, P. / Chmelik, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nan.cif.gz | 425.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2nan.ent.gz | 357 KB | Display | PDB format |
PDBx/mmJSON format | 2nan.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2nan_validation.pdf.gz | 544 KB | Display | wwPDB validaton report |
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Full document | 2nan_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 2nan_validation.xml.gz | 102.3 KB | Display | |
Data in CIF | 2nan_validation.cif.gz | 106.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/na/2nan ftp://data.pdbj.org/pub/pdb/validation_reports/na/2nan | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16227.978 Da / Num. of mol.: 1 / Fragment: Extracellular domain residues 23-161 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD302, CLEC13A, DCL1, KIAA0022 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-Gold(DE3) / References: UniProt: Q8IX05 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5 mM [U-100% 13C; U-100% 15N] CD302, 25 mM PIPES, 50 mM sodium chloride, 1 mM sodium azide, 90 % H2O, 10 % D2O, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||||||
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Sample |
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Sample conditions | pH: 6.5 / Pressure: 1 atm / Temperature: 303.15 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 600 MHz |
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-Processing
NMR software |
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