+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4an5 | ||||||
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タイトル | Capsid structure and its Stability at the Late Stages of Bacteriophage SPP1 Assembly | ||||||
要素 | COAT PROTEIN | ||||||
キーワード | VIRUS (ウイルス) / BACTERIOPHAGE CAPSID SPP1 | ||||||
機能・相同性 | Major capsid protein 13-like / Major capsid protein 13-like / T=7 icosahedral viral capsid / カプシド / Major capsid protein 機能・相同性情報 | ||||||
生物種 | BACILLUS PHAGE SPP1 (ファージ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 8.8 Å | ||||||
Model type details | CA ATOMS ONLY, CHAIN A, B, C, D, E, F, G | ||||||
データ登録者 | White, H.E. / Sherman, M.B. / Brasiles, S. / Jacquet, E. / Seavers, P. / Tavares, P. / Orlova, E.V. | ||||||
引用 | ジャーナル: J Virol / 年: 2012 タイトル: Capsid structure and its stability at the late stages of bacteriophage SPP1 assembly. 著者: Helen E White / Michael B Sherman / Sandrine Brasilès / Eric Jacquet / Philippa Seavers / Paulo Tavares / Elena V Orlova / 要旨: The structure of the bacteriophage SPP1 capsid was determined at subnanometer resolution by cryo-electron microscopy and single-particle analysis. The icosahedral capsid is composed of the major ...The structure of the bacteriophage SPP1 capsid was determined at subnanometer resolution by cryo-electron microscopy and single-particle analysis. The icosahedral capsid is composed of the major capsid protein gp13 and the auxiliary protein gp12, which are organized in a T=7 lattice. DNA is arranged in layers with a distance of ~24.5 Å. gp12 forms spikes that are anchored at the center of gp13 hexamers. In a gp12-deficient mutant, the centers of hexamers are closed by loops of gp13 coming together to protect the SPP1 genome from the outside environment. The HK97-like fold was used to build a pseudoatomic model of gp13. Its structural organization remains unchanged upon tail binding and following DNA release. gp13 exhibits enhanced thermostability in the DNA-filled capsid. A remarkable convergence between the thermostability of the capsid and those of the other virion components was found, revealing that the overall architecture of the SPP1 infectious particle coevolved toward high robustness. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4an5.cif.gz | 61.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb4an5.ent.gz | 44.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4an5.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/an/4an5 ftp://data.pdbj.org/pub/pdb/validation_reports/an/4an5 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
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対称性 | 点対称性: (シェーンフリース記号: I (正20面体型対称)) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
非結晶学的対称性 (NCS) | NCS oper:
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