+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1rgr | ||||||
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タイトル | Cyclic Peptides Targeting PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability | ||||||
要素 |
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キーワード | STRUCTURAL PROTEIN/DE NOVO PROTEIN (構造) / PDZ1 domain / STRUCTURAL PROTEIN-DE NOVO PROTEIN COMPLEX (構造) | ||||||
機能・相同性 | 機能・相同性情報 RHO GTPases activate CIT / positive regulation of AMPA glutamate receptor clustering / neuronal ion channel clustering / P2Y1 nucleotide receptor binding / Neurexins and neuroligins / beta-1 adrenergic receptor binding / neuroligin family protein binding / structural constituent of postsynaptic density / proximal dendrite / receptor localization to synapse ...RHO GTPases activate CIT / positive regulation of AMPA glutamate receptor clustering / neuronal ion channel clustering / P2Y1 nucleotide receptor binding / Neurexins and neuroligins / beta-1 adrenergic receptor binding / neuroligin family protein binding / structural constituent of postsynaptic density / proximal dendrite / receptor localization to synapse / positive regulation of neuron projection arborization / regulation of grooming behavior / synaptic vesicle maturation / cerebellar mossy fiber / cellular response to potassium ion / protein localization to synapse / vocalization behavior / LGI-ADAM interactions / Trafficking of AMPA receptors / neuron spine / 樹状突起 / Activation of Ca-permeable Kainate Receptor / AMPA glutamate receptor clustering / juxtaparanode region of axon / establishment or maintenance of epithelial cell apical/basal polarity / dendritic spine morphogenesis / frizzled binding / negative regulation of receptor internalization / postsynaptic neurotransmitter receptor diffusion trapping / neuron projection terminus / dendritic spine organization / acetylcholine receptor binding / positive regulation of synapse assembly / RAF/MAP kinase cascade / Synaptic adhesion-like molecules / neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of dendrite morphogenesis / beta-2 adrenergic receptor binding / cortical cytoskeleton / regulation of neuronal synaptic plasticity / locomotory exploration behavior / regulation of NMDA receptor activity / social behavior / positive regulation of excitatory postsynaptic potential / kinesin binding / AMPA glutamate receptor complex / neuromuscular process controlling balance / excitatory synapse / D1 dopamine receptor binding / Unblocking of NMDA receptors, glutamate binding and activation / glutamate receptor binding / positive regulation of protein tyrosine kinase activity / positive regulation of synaptic transmission / ionotropic glutamate receptor binding / extrinsic component of cytoplasmic side of plasma membrane / dendrite cytoplasm / synaptic membrane / PDZ domain binding / cell periphery / postsynaptic density membrane / regulation of long-term neuronal synaptic plasticity / neuromuscular junction / establishment of protein localization / 細胞接着 / cerebral cortex development / kinase binding / cell-cell junction / シナプス小胞 / 細胞結合 / positive regulation of cytosolic calcium ion concentration / chemical synaptic transmission / postsynapse / scaffold protein binding / postsynaptic membrane / basolateral plasma membrane / protein phosphatase binding / protein-containing complex assembly / 樹状突起スパイン / postsynaptic density / neuron projection / signaling receptor binding / 樹状突起 / シナプス / glutamatergic synapse / protein-containing complex binding / protein kinase binding / 小胞体 / 生体膜 / 細胞膜 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||
手法 | 溶液NMR / torsion angle dynamics, cartesian MD in water | ||||||
データ登録者 | Piserchio, A. / Salinas, G.D. / Li, T. / Marshall, J. / Spaller, M.R. / Mierke, D.F. | ||||||
引用 | ジャーナル: Chem.Biol. / 年: 2004 タイトル: Targeting Specific PDZ Domains of PSD-95; Structural Basis for Enhanced Affinity and Enzymatic Stability of a Cyclic Peptide. 著者: Piserchio, A. / Salinas, G.D. / Li, T. / Marshall, J. / Spaller, M.R. / Mierke, D.F. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1rgr.cif.gz | 654.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1rgr.ent.gz | 566.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1rgr.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/rg/1rgr ftp://data.pdbj.org/pub/pdb/validation_reports/rg/1rgr | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10860.138 Da / 分子数: 1 / 断片: PDZ1 domain of PSD-95 / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: DLG4, PSD95 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P31016 |
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#2: タンパク質・ペプチド | 分子量: 768.897 Da / 分子数: 1 / 断片: C-terminus / Mutation: Q98K, S100E / 由来タイプ: 合成 / 詳細: The peptide was chemically synthesized. / 参照: GenBank: 3098551 |
#3: 化合物 | ChemComp-BAL / |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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-試料調製
詳細 | 内容: 1mM PZ1 U-15N,13C, 3mM peptide, 10 mM phospate buffer 150 mM NaCl, pH 6.8 溶媒系: 90% H2O/10% D2O |
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-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: torsion angle dynamics, cartesian MD in water / ソフトェア番号: 1 | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with acceptable covalent geometry,structures with the least restraint violations 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 22 |