登録情報 データベース : EMDB / ID : EMD-21689 構造の表示 ダウンロードとリンクタイトル The cryo-EM structure of the human DNMT3A2-DNMT3B3 complex bound to NCP_Kc36me3. マップデータThe cryo-EM of human DNMT3A2/3B3 with NCP_Kc36me3. 詳細 試料複合体 : A ternary complex of DNMT3A/B asymmetrically binds to the NCP_Kc36me3複合体 : Histone ヒストン複合体 : DNA (167-MER)複合体 : DNA (cytosine-5)-methyltransferase 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
: / positive regulation of cellular response to hypoxia / DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates / : / epigenetic programming of gene expression / cellular response to bisphenol A / protein-cysteine methyltransferase activity / DNA-methyltransferase activity / ゲノム刷り込み / DNAメチルトランスフェラーゼ ... : / positive regulation of cellular response to hypoxia / DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates / : / epigenetic programming of gene expression / cellular response to bisphenol A / protein-cysteine methyltransferase activity / DNA-methyltransferase activity / ゲノム刷り込み / DNAメチルトランスフェラーゼ / unmethylated CpG binding / DNA (cytosine-5-)-methyltransferase activity / autosome genomic imprinting / XY body / DNA methylation-dependent heterochromatin formation / SUMOylation of DNA methylation proteins / cellular response to ethanol / response to vitamin A / : / response to ionizing radiation / hepatocyte apoptotic process / chromosome, centromeric region / catalytic complex / ヘテロクロマチン / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / DNAメチル化 / response to cocaine / PRC2 methylates histones and DNA / Defective pyroptosis / cellular response to amino acid stimulus / response to lead ion / ユークロマチン / NoRC negatively regulates rRNA expression / neuron differentiation / response to toxic substance / RMTs methylate histone arginines / nuclear matrix / structural constituent of chromatin / transcription corepressor activity / ヌクレオソーム / response to estradiol / cellular response to hypoxia / 精子形成 / RNA polymerase II-specific DNA-binding transcription factor binding / response to xenobiotic stimulus / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein heterodimerization activity / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of gene expression / negative regulation of transcription by RNA polymerase II / DNA binding / 核質 / identical protein binding / metal ion binding / 細胞核 / 細胞質 類似検索 - 分子機能 DNA (cytosine-5)-methyltransferase 3A, ADD domain / DNA (cytosine-5)-methyltransferase 3B, ADD domain / : / DNMT3, ADD PHD zinc finger / DNMT3, cysteine rich ADD domain / Cysteine rich ADD domain in DNMT3 / ADD domain / ADD domain profile. / DNA methylase, C-5 cytosine-specific, active site / C-5 cytosine-specific DNA methylases active site. ... DNA (cytosine-5)-methyltransferase 3A, ADD domain / DNA (cytosine-5)-methyltransferase 3B, ADD domain / : / DNMT3, ADD PHD zinc finger / DNMT3, cysteine rich ADD domain / Cysteine rich ADD domain in DNMT3 / ADD domain / ADD domain profile. / DNA methylase, C-5 cytosine-specific, active site / C-5 cytosine-specific DNA methylases active site. / C-5 cytosine-specific DNA methylase (Dnmt) domain profile. / C-5 cytosine methyltransferase / C-5 cytosine-specific DNA methylase / domain with conserved PWWP motif / PWWP domain / PWWP domain profile. / PWWP domain / Histone H2B signature. / ヒストンH2B / ヒストンH2B / Histone H2A conserved site / Histone H2A signature. / Histone H2A, C-terminal domain / C-terminus of histone H2A / ヒストンH2A / Histone 2A / Histone H4, conserved site / Histone H4 signature. / ヒストンH4 / ヒストンH4 / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Histone H3 signature 1. / Histone H3 signature 2. / ヒストンH3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Zinc finger, FYVE/PHD-type / Histone-fold / S-adenosyl-L-methionine-dependent methyltransferase superfamily 類似検索 - ドメイン・相同性 Histone H2B 1.1 / Histone H2A type 1 / ヒストンH4 / Histone H3.2 / DNA (cytosine-5)-methyltransferase 3B / DNMT3A 類似検索 - 構成要素生物種 Xenopus laevis (アフリカツメガエル) / synthetic construct (人工物) / Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4.26 Å 詳細 データ登録者Xu TH / Liu M / Zhou XE / Liang G / Zhao G / Xu HE / Melcher K / Jones PA 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Cancer Institute (NIH/NCI) R35CA209859 米国
引用ジャーナル : Nature / 年 : 2020タイトル : Structure of nucleosome-bound DNA methyltransferases DNMT3A and DNMT3B.著者 : Ting-Hai Xu / Minmin Liu / X Edward Zhou / Gangning Liang / Gongpu Zhao / H Eric Xu / Karsten Melcher / Peter A Jones / 要旨 : CpG methylation by de novo DNA methyltransferases (DNMTs) 3A and 3B is essential for mammalian development and differentiation and is frequently dysregulated in cancer. These two DNMTs preferentially ... CpG methylation by de novo DNA methyltransferases (DNMTs) 3A and 3B is essential for mammalian development and differentiation and is frequently dysregulated in cancer. These two DNMTs preferentially bind to nucleosomes, yet cannot methylate the DNA wrapped around the nucleosome core, and they favour the methylation of linker DNA at positioned nucleosomes. Here we present the cryo-electron microscopy structure of a ternary complex of catalytically competent DNMT3A2, the catalytically inactive accessory subunit DNMT3B3 and a nucleosome core particle flanked by linker DNA. The catalytic-like domain of the accessory DNMT3B3 binds to the acidic patch of the nucleosome core, which orients the binding of DNMT3A2 to the linker DNA. The steric constraints of this arrangement suggest that nucleosomal DNA must be moved relative to the nucleosome core for de novo methylation to occur. 履歴 登録 2020年4月12日 - ヘッダ(付随情報) 公開 2020年6月17日 - マップ公開 2020年6月17日 - 更新 2020年10月14日 - 現状 2020年10月14日 処理サイト : RCSB / 状態 : 公開
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