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- SASDBC3: RepB, the replication initiator protein of a promiscuous Streptoc... -

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Basic information

Entry
Database: SASBDB / ID: SASDBC3
SampleRepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158
  • Replication initiator protein of a promiscuous streptococcal plasmid pMV158. (protein), RepB, Streptococcus sp.
Biological speciesStreptococcus sp. (bacteria)
CitationDate: 2016 Feb 15
Title: Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158
Authors: Boer D / Ruiz-Masó J / Rueda M / Petoukhov M / Machón C / Svergun D / Orozco M / del Solar G
Contact author
  • Maxim Petoukhov (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #429
Type: mix / Software: OLIGOMER / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)
Model #430
Type: mix / Software: OLIGOMER / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)
Model #431
Type: mix / Software: OLIGOMER / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)
Model #432
Type: mix / Software: OLIGOMER / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158
BufferName: TRIS / Concentration: 10.00 mM / pH: 8.5 / Composition: 5 mM EDTA, 1.0 M KCl
Entity #292Name: RepB / Type: protein
Description: Replication initiator protein of a promiscuous streptococcal plasmid pMV158.
Num. of mol.: 6 / Source: Streptococcus sp.

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Experimental information

BeamInstrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron
DetectorName: MAR 345 Image Plate
Scan
Title: Replication initiator protein, RepB. / Measurement date: Jun 16, 2007 / Unit: 1/nm /
MinMax
Q0.1701 5.0544
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1016 /
MinMax
Q0.181732 2.53351
P(R) point1 1016
R0 10.68
Result
Type of curve: single_conc /
ExperimentalPorod
MW177 kDa-
Volume-282 nm3

P(R)P(R) errorGuinier
Forward scattering, I03.72 387 388.4
Radius of gyration, Rg--3.79 nm

MinMax
D-10.6
Guinier point6 75

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