+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDGK5 |
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試料 | The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (Paused SEC)
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機能・相同性 | 機能・相同性情報 LGI-ADAM interactions / P2Y1 nucleotide receptor binding / beta-1 adrenergic receptor binding / neuroligin family protein binding / NrCAM interactions / positive regulation of neuron projection arborization / synaptic vesicle maturation / regulation of grooming behavior / receptor localization to synapse / cellular response to potassium ion ...LGI-ADAM interactions / P2Y1 nucleotide receptor binding / beta-1 adrenergic receptor binding / neuroligin family protein binding / NrCAM interactions / positive regulation of neuron projection arborization / synaptic vesicle maturation / regulation of grooming behavior / receptor localization to synapse / cellular response to potassium ion / protein localization to synapse / cerebellar mossy fiber / vocalization behavior / Synaptic adhesion-like molecules / Signaling by ERBB4 / neuron spine / AMPA glutamate receptor clustering / Trafficking of AMPA receptors / dendritic spine morphogenesis / juxtaparanode region of axon / establishment or maintenance of epithelial cell apical/basal polarity / negative regulation of receptor internalization / postsynaptic neurotransmitter receptor diffusion trapping / neuron projection terminus / acetylcholine receptor binding / RHO GTPases activate CIT / Assembly and cell surface presentation of NMDA receptors / Neurexins and neuroligins / regulation of NMDA receptor activity / Activation of Ca-permeable Kainate Receptor / neurotransmitter receptor localization to postsynaptic specialization membrane / cortical cytoskeleton / Negative regulation of NMDA receptor-mediated neuronal transmission / Unblocking of NMDA receptors, glutamate binding and activation / locomotory exploration behavior / extrinsic component of cytoplasmic side of plasma membrane / positive regulation of excitatory postsynaptic potential / Long-term potentiation / AMPA glutamate receptor complex / social behavior / neuromuscular process controlling balance / positive regulation of protein tyrosine kinase activity / excitatory synapse / D1 dopamine receptor binding / positive regulation of synaptic transmission / ionotropic glutamate receptor binding / Ras activation upon Ca2+ influx through NMDA receptor / dendrite cytoplasm / learning / synaptic membrane / PDZ domain binding / adherens junction / kinase binding / cell-cell adhesion / postsynaptic density membrane / regulation of long-term neuronal synaptic plasticity / establishment of protein localization / neuromuscular junction / cell junction / endocytic vesicle membrane / synaptic vesicle / nervous system development / RAF/MAP kinase cascade / positive regulation of cytosolic calcium ion concentration / basolateral plasma membrane / protein phosphatase binding / scaffold protein binding / protein-containing complex assembly / chemical synaptic transmission / postsynaptic membrane / dendritic spine / postsynaptic density / neuron projection / glutamatergic synapse / synapse / protein-containing complex binding / protein kinase binding / signal transduction / endoplasmic reticulum / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | 日付: 2019 Sep 19 タイトル: How the dual PDZ domain from Postsynaptic density protein 95 clusters ion channels and receptors 著者: Rodzli N / Lockhart-Cairns M / Levy C / Chipperfield J / Bird L / Baldock C |
登録者 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #3769 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #3770 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3771 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3772 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3773 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3774 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3775 | タイプ: atomic / カイ2乗値: 4.21 / P-value: 0.005239 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (Paused SEC) 試料濃度: 7.5 mg/ml |
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バッファ | 名称: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM GSH / pH: 8.5 |
要素 #1889 | 名称: PDZ1-2 / タイプ: protein / 記述: PDZ1-2 fragment of PSD-95/Disks large homolog 4 / 分子量: 20.8 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P78352 配列: GPGTEGEMEY EEITLERGNS GLGFSIAGGT DNPHIGDDPS IFITKIIPGG AAAQDGRLRV NDSILFVNEV DVREVTHSAA VEALKEAGSI VRLYVMRRKP PAEKVMEIKL IKGPKGLGFS IAGGVGNQHI PGDNSIYVTK IIEGGAAHKD GRLQIGDKIL AVNSVGLEDV ...配列: GPGTEGEMEY EEITLERGNS GLGFSIAGGT DNPHIGDDPS IFITKIIPGG AAAQDGRLRV NDSILFVNEV DVREVTHSAA VEALKEAGSI VRLYVMRRKP PAEKVMEIKL IKGPKGLGFS IAGGVGNQHI PGDNSIYVTK IIEGGAAHKD GRLQIGDKIL AVNSVGLEDV MHEDAVAALK NTYDVVYLKV AKPSNA |
-実験情報
ビーム | 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 3.9 mm | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||
スキャン | タイトル: The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (Paused SEC) 測定日: 2016年1月26日 / 照射時間: 10 sec. / フレーム数: 69 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 1268 /
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結果 | カーブのタイプ: sec コメント: Scattering data are fitted using the ATSAS OLIGOMER program using a suite of models. The ATSAS program FFMAKER was used to generate form factors for each model in the suite. Each model ...コメント: Scattering data are fitted using the ATSAS OLIGOMER program using a suite of models. The ATSAS program FFMAKER was used to generate form factors for each model in the suite. Each model is assigned a volume fraction to fit the observed scattering profile. 3 monomer models are included, the first is a compact conformation of PDZ1-2 similar to PDB entries 6spv/6spz; the other two are domain models obtained from a representative run of the ATSAS EOM program with data projected to infinite dilution. Multimeric models are drawn from a "clustering Spacegroup", unit cell 14.8nm symmetry I2(1)3, and consist of identical copies of an extended conformation of PDZ1-2 (similar to PDB entry 3zrt) assembled by symmetry operations. In the order of deposition: Model number; Stoichiometry; MW (kDa); source; Volume fraction. 1; 1; 21; Crystal Structure; 0.318. 2; 1; 21; EOM; 0.088. 3; 1; 21; EOM; 0.500. 4; 2; 42; clustering Spacegroup; 0.074. 5; 4; 84; clustering Spacegroup; 0.009. 6; 8; 168; clustering Spacegroup; 0.011. 7; 12, 252; clustering Spacegroup; 0.000.
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