SASDBR9: Human fibroblast growth factor receptor 3, from SEC-SAXS

Basic information

• Entry: Database: SASBDB / ID: SASDBR9

Sample

Human fibroblast growth factor receptor 3, from SEC-SAXS

• Fibroblast growth factor receptor 3 (protein), FGFR3Fibroblast growth factor receptor 3, Homo sapiens

Function / homology

Function:

fibroblast growth factor receptor apoptotic signaling pathway / t(4;14) translocations of FGFR3 / negative regulation of developmental growth / Signaling by FGFR3 fusions in cancer / bone maturation / chondrocyte proliferation / endochondral bone growth / FGFR3b ligand binding and activation / Signaling by activated point mutants of FGFR3 / FGFR3c ligand binding and activation / Phospholipase C-mediated cascade; FGFR3 / fibroblast growth factor receptor activity / endochondral ossification / positive regulation of phospholipase activity / bone morphogenesis / PI-3K cascade:FGFR3 / fibroblast growth factor binding / bone mineralization / cell surface receptor signaling pathway via JAK-STAT / PI3K Cascade / fibroblast growth factor receptor signaling pathway / chondrocyte differentiation / SHC-mediated cascade:FGFR3 / FRS-mediated FGFR3 signaling / positive regulation of tyrosine phosphorylation of STAT protein / transport vesicle / Signaling by FGFR3 in disease / skeletal system development / Negative regulation of FGFR3 signaling / cell surface receptor protein tyrosine kinase signaling pathway / receptor protein-tyrosine kinase / Constitutive Signaling by Aberrant PI3K in Cancer / MAPK cascade / PIP3 activates AKT signaling / cell-cell signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / protein tyrosine kinase activity / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of ERK1 and ERK2 cascade / receptor complex / phosphorylation / positive regulation of cell population proliferation / Golgi apparatus / cell surface / endoplasmic reticulum / extracellular region / ATP binding / identical protein binding / plasma membrane

Domain/homology:

Fibroblast growth factor receptor 3 transmembrane domain / Fibroblast growth factor receptor family / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Immunoglobulin subtype / Immunoglobulin / Tyrosine-protein kinase, active site / Protein tyrosine and serine/threonine kinase / Serine-threonine/tyrosine-protein kinase, catalytic domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Immunoglobulin-like fold / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily

Component:

Fibroblast growth factor receptor 3

• Biological species: Homo sapiens (human)
• Citation: Date: 2018 Mar; Title: Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System; Authors: Bunney T / Inglis A / Sanfelice D / Farrell B / Kerr C / Thompson G / Masson G / Thiyagarajan N / Svergun D / Williams R / Breeze A

Contact author

• Chris Kerr (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #1134: ; Type: mix / Software: (ATSAS2.7.2) / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 3.10; Search similar-shape structures of this assembly by Omokage search (details)
• Model #1135: ; Type: dummy / Software: (ATSAS2.7.2) / Radius of dummy atoms: 3.00 A / Symmetry: P1 / Chi-square value: 1.201; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Human fibroblast growth factor receptor 3, from SEC-SAXS; Specimen concentration: 2 mg/ml
• Buffer: Name: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP; pH: 8

Entity #589

Name: FGFR3Fibroblast growth factor receptor 3 / Type: protein / Description: Fibroblast growth factor receptor 3 / Formula weight: 35.478 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P22607
Sequence:

SELELPADPK WELSRARLTL GKPLGEGAFG QVVMAEAIGI DKDRAAKPVT VAVKMLKDDA TDKDLSDLVS EMEMMKMIGK HKNFINLLGA CTQGGPLYVL VEYAAKGNLR EFLRARRPPG LDYSFDTSKP PEEQLTFKDL VSCAYQVARG MEYLASQKCI HRDLAARNVL VTEDNVMKIA DFGLARDVHN LDYYKKTTNG RLPVKWMAPE ALFDRVYTHQ SDVWSFGVLL WEIFTLGGSP YPGIPVEELF KLLKEGHRMD KPANCTHDLY MIMRECWHAA PSQRPTFKQL VEDLDRVLTV TSTDEYLDLS APFE

Experimental information

• Beam: Instrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
• Detector: Name: Pilatus 2M

Scan

Title: Human fibroblast growth factor receptor 3, from SEC-SAXS
Measurement date: Jul 10, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 3600 / Unit: 1/nm /

	Min	Max
Q	0.0248	5.0307

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1739 /

	Min	Max
Q	0.236934	5.02519
P(R) point	1	1739
R	0	8.3

Result

Type of curve: other /

	Experimental	Porod
MW	29.3 kDa	29.3 kDa
Volume	-	59.45 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	183.1	0.3181	183.31	0.38
Radius of gyration, Rg	2.363 nm	0.007	2.35 nm	0.27

	Min	Max	Error
D	-	8.3	3
Guinier point	78	192	-