- PDB-8uk9: Structure of T4 Bacteriophage clamp loader mutant D110C bound to ... -
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基本情報
登録情報
データベース: PDB / ID: 8uk9
タイトル
Structure of T4 Bacteriophage clamp loader mutant D110C bound to the T4 clamp, primer-template DNA, and ATP analog
要素
(Sliding-clamp-loader ...) x 2
DNA primerプライマー (生物)
DNA template
Sliding clampDNAクランプ
キーワード
DNA BINDING PROTEIN/DNA / DNA Replication (DNA複製) / AAA+ ATPase / Bacteriophage (ファージ) / Complex / DNA (デオキシリボ核酸) / DNA BINDING PROTEIN (DNA結合タンパク質) / DNA BINDING PROTEIN-DNA complex
機能・相同性
機能・相同性情報
Rad17 RFC-like complex / Elg1 RFC-like complex / DNA replication factor C complex / DNA clamp loader activity / bidirectional double-stranded viral DNA replication / viral DNA genome replication / DNA polymerase processivity factor activity / viral transcription / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / DNA-templated DNA replication ...Rad17 RFC-like complex / Elg1 RFC-like complex / DNA replication factor C complex / DNA clamp loader activity / bidirectional double-stranded viral DNA replication / viral DNA genome replication / DNA polymerase processivity factor activity / viral transcription / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / DNA-templated DNA replication / DNA複製 / DNA修復 / ATP hydrolysis activity / DNA binding / ATP binding 類似検索 - 分子機能
Sliding-clamp-loader small subunit gp62 / Sliding-clamp-loader large subunit / : / : / Bacteriophage clamp loader A subunit / Sliding-clamp-loader large subunit, AAA+ ATPase lid domain / Sliding-clamp-loader large subunit, C-terminal domain / Sliding clamp, C-terminal / DNAクランプ / gp45 sliding clamp, C terminal ...Sliding-clamp-loader small subunit gp62 / Sliding-clamp-loader large subunit / : / : / Bacteriophage clamp loader A subunit / Sliding-clamp-loader large subunit, AAA+ ATPase lid domain / Sliding-clamp-loader large subunit, C-terminal domain / Sliding clamp, C-terminal / DNAクランプ / gp45 sliding clamp, C terminal / DNA polymerase processivity factor / DNA polymerase processivity factor / : / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
ADENOSINE-5'-DIPHOSPHATE / フッ化アルミニウム / デオキシリボ核酸 / DNA (> 10) / DNAクランプ / Sliding-clamp-loader large subunit / Sliding-clamp-loader small subunit 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2024 タイトル: Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM. 著者: Kendra Marcus / Yongjian Huang / Subu Subramanian / Christine L Gee / Kent Gorday / Sam Ghaffari-Kashani / Xiao Ran Luo / Lisa Zheng / Michael O'Donnell / Sriram Subramaniam / John Kuriyan / 要旨: Clamp loaders are AAA+ ATPases that facilitate high-speed DNA replication. In eukaryotic and bacteriophage clamp loaders, ATP hydrolysis requires interactions between aspartate residues in one ...Clamp loaders are AAA+ ATPases that facilitate high-speed DNA replication. In eukaryotic and bacteriophage clamp loaders, ATP hydrolysis requires interactions between aspartate residues in one protomer, present in conserved 'DEAD-box' motifs, and arginine residues in adjacent protomers. We show that functional defects resulting from a DEAD-box mutation in the T4 bacteriophage clamp loader can be compensated by widely distributed single mutations in the ATPase domain. Using cryo-EM, we discovered an unsuspected inactive conformation of the clamp loader, in which DNA binding is blocked and the catalytic sites are disassembled. Mutations that restore function map to regions of conformational change upon activation, suggesting that these mutations may increase DNA affinity by altering the energetic balance between inactive and active states. Our results show that there are extensive opportunities for evolution to improve catalytic efficiency when an inactive intermediate is involved.
A: Sliding-clamp-loader small subunit B: Sliding-clamp-loader large subunit C: Sliding-clamp-loader large subunit D: Sliding-clamp-loader large subunit E: Sliding-clamp-loader large subunit F: Sliding clamp G: Sliding clamp H: Sliding clamp I: DNA template J: DNA primer K: Sliding-clamp-loader large subunit L: Sliding-clamp-loader large subunit M: Sliding-clamp-loader large subunit N: Sliding-clamp-loader large subunit O: DNA template P: DNA primer Q: Sliding-clamp-loader small subunit R: Sliding clamp S: Sliding clamp T: Sliding clamp ヘテロ分子
A: Sliding-clamp-loader small subunit B: Sliding-clamp-loader large subunit C: Sliding-clamp-loader large subunit D: Sliding-clamp-loader large subunit E: Sliding-clamp-loader large subunit F: Sliding clamp G: Sliding clamp H: Sliding clamp I: DNA template J: DNA primer ヘテロ分子
K: Sliding-clamp-loader large subunit L: Sliding-clamp-loader large subunit M: Sliding-clamp-loader large subunit N: Sliding-clamp-loader large subunit O: DNA template P: DNA primer Q: Sliding-clamp-loader small subunit R: Sliding clamp S: Sliding clamp T: Sliding clamp ヘテロ分子