+Open data
-Basic information
Entry | Database: PDB / ID: 8spr | ||||||||||||
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Title | The cryo-EM structure of the EcBAM/EspP(beta1-12) complex | ||||||||||||
Components |
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Keywords | MEMBRANE PROTEIN/Hydrolase / membrane proteins / protein folding / BAM complex / outer membrane protein / MEMBRANE PROTEIN / MEMBRANE PROTEIN-Hydrolase complex | ||||||||||||
Function / homology | Function and homology information Bam protein complex / Gram-negative-bacterium-type cell outer membrane assembly / protein insertion into membrane / Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases / cell outer membrane / periplasmic space / serine-type endopeptidase activity / cell surface / proteolysis / extracellular region ...Bam protein complex / Gram-negative-bacterium-type cell outer membrane assembly / protein insertion into membrane / Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases / cell outer membrane / periplasmic space / serine-type endopeptidase activity / cell surface / proteolysis / extracellular region / membrane / identical protein binding Similarity search - Function | ||||||||||||
Biological species | Escherichia coli (E. coli) Escherichia coli 0.1288 (bacteria) Escherichia coli O157 (bacteria) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å | ||||||||||||
Authors | Wu, R. / Noinaj, N. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: To Be Published Title: BAM orchestrates OMP biogenesis using a beta-templating mechanism Authors: Wu, R. / Ryoo, D. / Kuo, K.M. / Gumbart, J.C. / Noinaj, N. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8spr.cif.gz | 351.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8spr.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8spr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sp/8spr ftp://data.pdbj.org/pub/pdb/validation_reports/sp/8spr | HTTPS FTP |
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-Related structure data
Related structure data | 40682MC 8sqaC 8sqbC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Outer membrane protein assembly factor ... , 5 types, 5 molecules ABCDE
#1: Protein | Mass: 88247.477 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: bamA / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: C3TPJ2 |
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#2: Protein | Mass: 39779.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: bamB, yfgL, b2512, JW2496 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P77774 |
#3: Protein | Mass: 34298.109 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli 0.1288 (bacteria) / Gene: bamC, dapX, nlpB, b2477, JW2462 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0A903 |
#4: Protein | Mass: 25713.676 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: bamD / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: C3SYV7 |
#5: Protein | Mass: 10540.661 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: bamE, smpA / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A366UU94 |
-Protein , 1 types, 1 molecules F
#6: Protein | Mass: 38773.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli O157 (bacteria) / Gene: espP, L7020, ECO57PM78 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: Q7BSW5, Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: BAM/EspP(beta1-12) / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Escherichia coli (E. coli) |
Source (recombinant) | Organism: Escherichia coli BL21(DE3) (bacteria) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE / Humidity: 100 % |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm |
Image recording | Electron dose: 53.47 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software | Name: PHENIX / Version: 1.18rc1_3777: / Category: model refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 145290 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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