- PDB-8p0f: Crystal structure of the VCB complex with compound 1. -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 8p0f
Title
Crystal structure of the VCB complex with compound 1.
Components
Elongin-B
Elongin-C
von Hippel-Lindau disease tumor suppressor
Keywords
LIGASE / Inhibitor / Ligand / PROTAC
Function / homology
Function and homology information
regulation of cellular response to hypoxia / RHOBTB3 ATPase cycle / negative regulation of receptor signaling pathway via JAK-STAT / transcription elongation factor activity / target-directed miRNA degradation / elongin complex / VCB complex / Replication of the SARS-CoV-1 genome / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex ...regulation of cellular response to hypoxia / RHOBTB3 ATPase cycle / negative regulation of receptor signaling pathway via JAK-STAT / transcription elongation factor activity / target-directed miRNA degradation / elongin complex / VCB complex / Replication of the SARS-CoV-1 genome / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / intracellular non-membrane-bounded organelle / SUMOylation of ubiquitinylation proteins / negative regulation of transcription elongation by RNA polymerase II / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / ubiquitin-like ligase-substrate adaptor activity / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / negative regulation of signal transduction / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / negative regulation of TORC1 signaling / RNA Polymerase II Pre-transcription Events / negative regulation of autophagy / transcription corepressor binding / Evasion by RSV of host interferon responses / transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / positive regulation of cell differentiation / TP53 Regulates Transcription of DNA Repair Genes / Vif-mediated degradation of APOBEC3G / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / cell morphogenesis / Inactivation of CSF3 (G-CSF) signaling / Regulation of expression of SLITs and ROBOs / ubiquitin-protein transferase activity / transcription corepressor activity / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / protein-macromolecule adaptor activity / Neddylation / Replication of the SARS-CoV-2 genome / cellular response to hypoxia / ubiquitin-dependent protein catabolic process / regulation of gene expression / proteasome-mediated ubiquitin-dependent protein catabolic process / protein-containing complex assembly / DNA-binding transcription factor binding / amyloid fibril formation / molecular adaptor activity / protein stabilization / protein ubiquitination / negative regulation of cell population proliferation / negative regulation of gene expression / ubiquitin protein ligase binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / mitochondrion / proteolysis / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function
von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin B / Elongin-C ...von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin B / Elongin-C / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily Similarity search - Domain/homology
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.98→35.68 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.909 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.401 / SU Rfree Blow DPI: 0.248 Details: HYDROGENS WERE FULLY REFINED WITH ZERO OCCUPANCY AT NUCLEAR POSITION.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2565
1680
5.01 %
RANDOM
Rwork
0.2288
-
-
-
obs
0.2302
33550
58.1 %
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Displacement parameters
Biso mean: 58.92 Å2
Baniso -1
Baniso -2
Baniso -3
1-
8.3305 Å2
2.1832 Å2
2.108 Å2
2-
-
-2.9881 Å2
0.9599 Å2
3-
-
-
-5.3424 Å2
Refine analyze
Luzzati coordinate error obs: 0.33 Å
Refinement step
Cycle: LAST / Resolution: 1.98→35.68 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
5438
0
61
329
5828
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
Restraint function
Weight
X-RAY DIFFRACTION
t_bond_d
0.008
11112
HARMONIC
2
X-RAY DIFFRACTION
t_angle_deg
0.95
20135
HARMONIC
2
X-RAY DIFFRACTION
t_dihedral_angle_d
3283
SINUSOIDAL
2
X-RAY DIFFRACTION
t_incorr_chiral_ct
X-RAY DIFFRACTION
t_pseud_angle
X-RAY DIFFRACTION
t_trig_c_planes
X-RAY DIFFRACTION
t_gen_planes
1717
HARMONIC
5
X-RAY DIFFRACTION
t_it
5625
HARMONIC
10
X-RAY DIFFRACTION
t_nbd
X-RAY DIFFRACTION
t_omega_torsion
2.99
X-RAY DIFFRACTION
t_other_torsion
15.8
X-RAY DIFFRACTION
t_improper_torsion
X-RAY DIFFRACTION
t_chiral_improper_torsion
730
SEMIHARMONIC
5
X-RAY DIFFRACTION
t_sum_occupancies
X-RAY DIFFRACTION
t_utility_distance
X-RAY DIFFRACTION
t_utility_angle
X-RAY DIFFRACTION
t_utility_torsion
X-RAY DIFFRACTION
t_ideal_dist_contact
9089
SEMIHARMONIC
4
LS refinement shell
Resolution: 1.98→2.16 Å / Total num. of bins used: 51
Rfactor
Num. reflection
% reflection
Rfree
0.335
-
4.92 %
Rwork
0.2886
638
-
all
0.2908
671
-
obs
-
-
5.07 %
Refinement TLS params.
Method: refined / Refine-ID: X-RAY DIFFRACTION
ID
L11 (°2)
L12 (°2)
L13 (°2)
L22 (°2)
L23 (°2)
L33 (°2)
S11 (Å °)
S12 (Å °)
S13 (Å °)
S21 (Å °)
S22 (Å °)
S23 (Å °)
S31 (Å °)
S32 (Å °)
S33 (Å °)
T11 (Å2)
T12 (Å2)
T13 (Å2)
T22 (Å2)
T23 (Å2)
T33 (Å2)
Origin x (Å)
Origin y (Å)
Origin z (Å)
1
6.4362
-0.9186
-1.4794
0.3869
0.3942
0.8477
-0.5446
-0.4399
-0.0269
0.0473
0.2723
-0.0696
-0.0061
0.0211
0.2722
0.1513
0.1601
0.0905
-0.0724
0.0097
-0.1535
12.5931
-23.7796
37.9772
2
5.0172
0.7093
0.3543
1.7445
0.0731
2.6055
-0.6935
0.4035
0.344
-0.0878
0.3729
0.0818
-0.2048
0.1937
0.3206
0.1758
-0.0694
-0.014
-0.1223
0.0371
-0.1624
32.8596
-14.0764
22.1464
3
3.5432
-1.0585
0.1484
2.7087
-2.2009
3.0402
-0.5656
1.1835
0.4744
-0.2566
0.2924
0.2584
-0.0235
-0.066
0.2731
0.112
-0.3018
-0.1801
0.0755
0.212
-0.2259
28.0519
-8.4593
5.861
4
1.0748
0.4596
0.5731
7.2715
-1.865
1.791
0.0866
0.0911
0.0241
-0.3964
0.0169
0.1237
-0.0652
0.1186
-0.1034
0.0459
0.0743
0.0341
-0.1083
-0.0233
-0.1099
20.6317
-9.2606
-33.4831
5
2.6863
1.2994
-0.0733
4.6515
1.4851
3.8601
0.3244
-0.0514
-0.0812
0.3265
-0.2891
0.0818
0.3164
-0.3253
-0.0353
0.0837
-0.0026
-0.0753
-0.1265
0.0274
-0.1245
12.3566
-29.2205
-15.8644
6
2.3071
-1.3354
-2.25
1.9562
2.2686
5.0371
0.365
-0.175
0.0112
0.7694
-0.3949
-0.1418
0.4425
-0.1423
0.0299
0.2072
-0.231
-0.0706
-0.0645
0.0494
-0.255
11.4507
-24.7251
1.4573
7
0.3501
0.0973
-0.5212
0.799
0.3603
0.1274
-0.1053
-0.0347
0.0165
-0.0061
0.1024
0.0477
-0.0358
-0.022
0.0029
-0.1998
0.0432
0.0205
-0.1151
0.1079
-0.1256
10.8964
-10.3704
2.0558
Refinement TLS group
ID
Refine-ID
Refine TLS-ID
Selection details
Auth asym-ID
Auth seq-ID
1
X-RAY DIFFRACTION
1
{ A|62 - A|207 }
A
62 - 207
2
X-RAY DIFFRACTION
2
{ B|16 - B|111 }
B
16 - 111
3
X-RAY DIFFRACTION
3
{ C|1 - C|104 }
C
1 - 104
4
X-RAY DIFFRACTION
4
{ D|62 - D|207 }
D
62 - 207
5
X-RAY DIFFRACTION
5
{ E|16 - E|111 }
E
16 - 111
6
X-RAY DIFFRACTION
6
{ F|1 - F|104 }
F
1 - 104
7
X-RAY DIFFRACTION
7
{ A|301 - A|301 D|301 - D|301 }
A
301
8
X-RAY DIFFRACTION
7
{ A|301 - A|301 D|301 - D|301 }
D
301
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