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Open data
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Basic information
Entry | Database: PDB / ID: 8owx | ||||||
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Title | Crystal Structure of METTL6 bound to SAH | ||||||
![]() | tRNA N(3)-methylcytidine methyltransferase METTL6 | ||||||
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Function / homology | ![]() tRNA (cytidine-3-)-methyltransferase activity / tRNA methylation / tRNA modification / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Throll, P. / Basu, S. / Dolce, L.G. / Kowalinski, E. | ||||||
Funding support | 1items
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![]() | ![]() Title: Structural basis of the cooperation between the m3C RNA-methyltransferase METTL6 with SerRS amino-acyl-synthetase for tRNA recognition Authors: Throll, P. / Dolce, L.G. / Lastres, P.R. / Arnold, K. / Tengo, L. / Kaiser, S. / Basu, S. / Schneider, R. / Kowalinski, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.9 KB | Display | ![]() |
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PDB format | ![]() | 86.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8owyC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32346.895 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q8TCB7, ![]() #2: Chemical | ![]() #3: Chemical | ChemComp-B3P / | ![]() Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.9 Å3/Da / Density % sol: 68.47 % |
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Crystal grow![]() | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M BisTRIS propane pH 6.5, 0.2 M sodium sulfate, 20 % PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Sep 21, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→41.43 Å / Num. obs: 26988 / % possible obs: 98.36 % / Redundancy: 13.6 % / Biso Wilson estimate: 80.78 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.2006 / Net I/σ(I): 9.43 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 13.3 % / Rmerge(I) obs: 4.8 / Num. unique obs: 2653 / CC1/2: 0.143 / % possible all: 83.61 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.601→41.43 Å
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Refine LS restraints |
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LS refinement shell |
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